| | | |
| March 7, 2020 | Louisville City FC 1 at North Carolina FC 0 | Final |
| March 14, 2020 | Philadelphia Union II at North Carolina FC | Ppd. |
| March 21, 2020 | North Carolina FC at Tampa Bay Rowdies | Ppd. |
| March 27, 2020 | Chattanooga Red Wolves SC at Greenville Triumph SC | Ppd. |
| March 27, 2020 | Union Omaha at Fort Lauderdale CF | Ppd. |
| March 28, 2020 | Orlando City B at New England Revolution II | Ppd. |
| March 28, 2020 | Forward Madison FC at Richmond Kickers | Ppd. |
| March 28, 2020 | North Texas SC at South Georgia Tormenta FC | Ppd. |
| March 28, 2020 | Toronto FC II at FC Tucson | Ppd. |
| April 3, 2020 | Richmond Kickers at New England Revolution II | Ppd. |
| April 3, 2020 | Charlotte Independence at North Carolina FC | Ppd. |
| April 4, 2020 | South Georgia Tormenta FC at Greenville Triumph SC | Ppd. |
| April 4, 2020 | Orlando City B at North Texas SC | Ppd. |
| April 4, 2020 | Forward Madison FC at FC Tucson | Ppd. |
| April 11, 2020 | North Carolina FC at Atlanta United 2 | Ppd. |
| April 11, 2020 | Chattanooga Red Wolves SC at Orlando City B | Ppd. |
| April 11, 2020 | Fort Lauderdale CF at South Georgia Tormenta FC | Ppd. |
| April 11, 2020 | Toronto FC II at Forward Madison FC | Ppd. |
| April 11, 2020 | North Texas SC at FC Tucson | Ppd. |
| April 12, 2020 | Greenville Triumph SC at New England Revolution II | Ppd. |
| April 15, 2020 | North Carolina FC at Sporting Kansas City II | Ppd. |
| April 17, 2020 | FC Tucson at Orlando City B | Ppd. |
| April 18, 2020 | Greenville Triumph SC at Richmond Kickers | Ppd. |
| April 18, 2020 | Toronto FC II at South Georgia Tormenta FC | Ppd. |
| April 18, 2020 | Union Omaha at Forward Madison FC | Ppd. |
| April 18, 2020 | Chattanooga Red Wolves SC at North Texas SC | Ppd. |
| April 19, 2020 | New England Revolution II at Fort Lauderdale CF | Ppd. |
| April 25, 2020 | Richmond Kickers at Chattanooga Red Wolves SC | Ppd. |
| April 25, 2020 | Birmingham Legion FC at North Carolina FC | Ppd. |
| April 25, 2020 | Toronto FC II at New England Revolution II | Ppd. |
| April 25, 2020 | Orlando City B at Union Omaha | Ppd. |
| April 26, 2020 | North Texas SC at Greenville Triumph SC | Ppd. |
| April 26, 2020 | Forward Madison FC at Fort Lauderdale CF | Ppd. |
| May 1, 2020 | Richmond Kickers at Toronto FC II | Ppd. |
| May 1, 2020 | Greenville Triumph SC at Orlando City B | Ppd. |
| May 2, 2020 | Pittsburgh Riverhounds SC at North Carolina FC | Ppd. |
| May 2, 2020 | New England Revolution II at South Georgia Tormenta FC | Ppd. |
| May 2, 2020 | Chattanooga Red Wolves SC at Forward Madison FC | Ppd. |
| May 2, 2020 | Fort Lauderdale CF at FC Tucson | Ppd. |
| May 3, 2020 | Union Omaha at North Texas SC | Ppd. |
| May 6, 2020 | Greenville Triumph SC at Toronto FC II | Ppd. |
| May 9, 2020 | South Georgia Tormenta FC at Richmond Kickers | Ppd. |
| May 9, 2020 | FC Tucson at Chattanooga Red Wolves SC | Ppd. |
| May 9, 2020 | Union Omaha at Greenville Triumph SC | Ppd. |
| May 9, 2020 | Memphis 901 FC at North Carolina FC | Ppd. |
| May 9, 2020 | Toronto FC II at Fort Lauderdale CF | Ppd. |
| May 9, 2020 | Forward Madison FC at North Texas SC | Ppd. |
| May 13, 2020 | North Carolina FC at Loudoun United FC | Ppd. |
| May 13, 2020 | Chattanooga Red Wolves SC at Fort Lauderdale CF | Ppd. |
| May 15, 2020 | Forward Madison FC at Toronto FC II | Ppd. |
| May 15, 2020 | Union Omaha at New England Revolution II | Ppd. |
| May 16, 2020 | North Carolina FC at Charleston Battery | Ppd. |
| May 16, 2020 | Richmond Kickers at Orlando City B | Ppd. |
| May 16, 2020 | Chattanooga Red Wolves SC at South Georgia Tormenta FC | Ppd. |
| May 17, 2020 | FC Tucson at Greenville Triumph SC | Ppd. |
| May 22, 2020 | Fort Lauderdale CF at New England Revolution II | Ppd. |
| May 23, 2020 | North Carolina FC at Indy Eleven | Ppd. |
| May 23, 2020 | Orlando City B at Chattanooga Red Wolves SC | Ppd. |
| May 23, 2020 | FC Tucson at Union Omaha | Ppd. |
| May 23, 2020 | South Georgia Tormenta FC at North Texas SC | Ppd. |
| May 27, 2020 | South Georgia Tormenta FC at Union Omaha | Ppd. |
| May 30, 2020 | North Texas SC at Richmond Kickers | Ppd. |
| May 30, 2020 | Toronto FC II at Chattanooga Red Wolves SC | Ppd. |
| May 30, 2020 | Fort Lauderdale CF at Greenville Triumph SC | Ppd. |
| May 30, 2020 | New York Red Bulls II at North Carolina FC | Ppd. |
| May 30, 2020 | New England Revolution II at Forward Madison FC | Ppd. |
| May 30, 2020 | Orlando City B at FC Tucson | Ppd. |
| June 2, 2020 | South Georgia Tormenta FC at New England Revolution II | Ppd. |
| June 3, 2020 | Richmond Kickers at Toronto FC II | Ppd. |
| June 5, 2020 | New England Revolution II at Orlando City B | Ppd. |
| June 6, 2020 | Forward Madison FC at Richmond Kickers | Ppd. |
| June 6, 2020 | Union Omaha at Chattanooga Red Wolves SC | Ppd. |
| June 6, 2020 | Hartford Athletic at North Carolina FC | Ppd. |
| June 6, 2020 | FC Tucson at Fort Lauderdale CF | Ppd. |
| June 6, 2020 | Greenville Triumph SC at South Georgia Tormenta FC | Ppd. |
| June 6, 2020 | Toronto FC II at North Texas SC | Ppd. |
| June 11, 2020 | Chattanooga Red Wolves SC at Greenville Triumph SC | Ppd. |
| June 12, 2020 | Fort Lauderdale CF at Toronto FC II | Ppd. |
| June 12, 2020 | North Texas SC at New England Revolution II | Ppd. |
| June 12, 2020 | North Carolina FC at Charlotte Independence | Ppd. |
| June 12, 2020 | South Georgia Tormenta FC at Orlando City B | Ppd. |
| June 12, 2020 | Richmond Kickers at Union Omaha | Ppd. |
| June 13, 2020 | Forward Madison FC at FC Tucson | Ppd. |
| June 17, 2020 | Union Omaha at FC Tucson | Ppd. |
| June 20, 2020 | Greenville Triumph SC at Richmond Kickers | Ppd. |
| June 20, 2020 | New England Revolution II at Chattanooga Red Wolves SC | Ppd. |
| June 20, 2020 | Saint Louis FC at North Carolina FC | Ppd. |
| June 20, 2020 | Toronto FC II at Forward Madison FC | Ppd. |
| June 20, 2020 | FC Tucson at South Georgia Tormenta FC | Ppd. |
| June 21, 2020 | Orlando City B at Fort Lauderdale CF | Ppd. |
| June 21, 2020 | Union Omaha at North Texas SC | Ppd. |
| June 26, 2020 | South Georgia Tormenta FC at Toronto FC II | Ppd. |
| June 27, 2020 | Orlando City B at Richmond Kickers | Ppd. |
| June 27, 2020 | Loudoun United FC at North Carolina FC | Ppd. |
| June 27, 2020 | Fort Lauderdale CF at Forward Madison FC | Ppd. |
| June 27, 2020 | New England Revolution II at Union Omaha | Ppd. |
| June 27, 2020 | Greenville Triumph SC at North Texas SC | Ppd. |
| June 27, 2020 | Chattanooga Red Wolves SC at FC Tucson | Ppd. |
| July 1, 2020 | Chattanooga Red Wolves SC at Toronto FC II | Ppd. |
| July 2, 2020 | Forward Madison FC at Greenville Triumph SC | Ppd. |
| July 2, 2020 | Union Omaha at Orlando City B | Ppd. |
| July 2, 2020 | Richmond Kickers at Fort Lauderdale CF | Ppd. |
| July 3, 2020 | New England Revolution II at FC Tucson | Ppd. |
| July 4, 2020 | North Carolina FC at Pittsburgh Riverhounds SC | Ppd. |
| July 5, 2020 | North Texas SC at South Georgia Tormenta FC | Ppd. |
| July 8, 2020 | Toronto FC II at New England Revolution II | Ppd. |
| July 11, 2020 | FC Tucson at Richmond Kickers | Ppd. |
| July 11, 2020 | South Georgia Tormenta FC at Chattanooga Red Wolves SC | Ppd. |
| July 11, 2020 | Greenville Triumph SC at Fort Lauderdale CF | Ppd. |
| July 11, 2020 | Union Omaha at Forward Madison FC | Ppd. |
| July 12, 2020 | New England Revolution II at North Texas SC | Ppd. |
| July 15, 2020 | Orlando City B at Forward Madison FC | Ppd. |
| July 17, 2020 | Fort Lauderdale CF at Toronto FC II | Ppd. |
| July 17, 2020 | North Carolina FC 2 at Tampa Bay Rowdies 2 | Final |
| July 17, 2020 | Richmond Kickers at Union Omaha | Ppd. |
| July 18, 2020 | Greenville Triumph SC 2 at Fort Lauderdale CF 0 | Final |
| July 18, 2020 | Richmond Kickers at South Georgia Tormenta FC | Ppd. |
| July 25, 2020 | Union Omaha 0 at New England Revolution II 0 | Final |
| July 25, 2020 | Richmond Kickers 2 at Greenville Triumph SC 3 | Final |
| July 25, 2020 | FC Tucson 2 at Fort Lauderdale CF 1 | Final |
| July 25, 2020 | Chattanooga Red Wolves SC 2 at South Georgia Tormenta FC 2 | Final |
| July 25, 2020 | Forward Madison FC 1 at North Texas SC 2 | Final |
| July 28, 2020 | Richmond Kickers 0 at South Georgia Tormenta FC 0 | Final |
| July 31, 2020 | Greenville Triumph SC 0 at Forward Madison FC 0 | Final |
| August 1, 2020 | FC Tucson 0 at Chattanooga Red Wolves SC 1 | Final |
| August 1, 2020 | Fort Lauderdale CF at Richmond Kickers | Ppd. |
| August 1, 2020 | Birmingham Legion FC 0 at North Carolina FC 1 | Final |
| August 1, 2020 | Orlando City B 0 at South Georgia Tormenta FC 2 | Final |
| August 1, 2020 | North Texas SC 0 at Union Omaha 1 | Final |
| August 5, 2020 | South Georgia Tormenta FC 0 at Greenville Triumph SC 1 | Final |
| August 7, 2020 | Orlando City B 2 at New England Revolution II 0 | Final |
| August 8, 2020 | North Carolina FC 1 at Memphis 901 FC 0 | Final |
| August 8, 2020 | Forward Madison FC 0 at Richmond Kickers 1 | Final |
| August 8, 2020 | Fort Lauderdale CF 2 at South Georgia Tormenta FC 1 | Final |
| August 8, 2020 | Chattanooga Red Wolves SC 2 at North Texas SC 2 | Final |
| August 8, 2020 | Union Omaha 2 at FC Tucson 1 | Final |
| August 12, 2020 | Union Omaha at Chattanooga Red Wolves SC | Ppd. |
| August 14, 2020 | Fort Lauderdale CF 1 at Orlando City B 1 | Final |
| August 14, 2020 | South Georgia Tormenta FC 0 at Forward Madison FC 4 | Final |
| August 15, 2020 | FC Tucson 1 at Richmond Kickers 2 | Final |
| August 15, 2020 | Chattanooga Red Wolves SC 0 at Greenville Triumph SC 1 | Final |
| August 15, 2020 | New England Revolution II 3 at North Texas SC 3 | Final |
| August 18, 2020 | FC Tucson 4 at Orlando City B 1 | Final |
| August 19, 2020 | Charleston Battery 3 at North Carolina FC 0 | Final |
| August 19, 2020 | North Texas SC 2 at Fort Lauderdale CF 3 | Final |
| August 19, 2020 | Forward Madison FC 1 at Union Omaha 1 | Final |
| August 21, 2020 | Richmond Kickers 2 at New England Revolution II 1 | Final |
| August 22, 2020 | South Georgia Tormenta FC 2 at Chattanooga Red Wolves SC 1 | Final |
| August 22, 2020 | Fort Lauderdale CF 0 at Greenville Triumph SC 1 | Final |
| August 23, 2020 | Orlando City B 1 at Forward Madison FC 3 | Final |
| August 23, 2020 | Union Omaha 2 at North Texas SC 2 | Final |
| August 26, 2020 | North Carolina FC 0 at Charlotte Independence 1 | Final |
| August 26, 2020 | Greenville Triumph SC 0 at New England Revolution II 1 | Final |
| August 28, 2020 | South Georgia Tormenta FC 1 at Orlando City B 1 | Final |
| August 29, 2020 | Memphis 901 FC at North Carolina FC | Ppd. |
| August 29, 2020 | New England Revolution II 0 at Chattanooga Red Wolves SC 4 | Final |
| August 29, 2020 | Greenville Triumph SC 1 at Richmond Kickers 2 | Final |
| August 29, 2020 | FC Tucson 1 at Union Omaha 2 | Final |
| August 30, 2020 | North Texas SC at Forward Madison FC | Ppd. |
| September 2, 2020 | North Carolina FC 2 at Birmingham Legion FC 1 | Final |
| September 3, 2020 | North Texas SC 1 at Orlando City B 1 | Final |
| September 4, 2020 | Forward Madison FC 4 at New England Revolution II 0 | Final |
| September 4, 2020 | Greenville Triumph SC 2 at South Georgia Tormenta FC 1 | Final |
| September 5, 2020 | North Carolina FC 1 at Charlotte Independence 3 | Final |
| September 5, 2020 | Chattanooga Red Wolves SC 1 at Fort Lauderdale CF 1 | Final |
| September 5, 2020 | Richmond Kickers 2 at FC Tucson 2 | Final |
| September 6, 2020 | Orlando City B 0 at Union Omaha 1 | Final |
| September 9, 2020 | Chattanooga Red Wolves SC 2 at New England Revolution II 1 | Final |
| September 10, 2020 | Memphis 901 FC 3 at North Carolina FC 2 | Final |
| September 11, 2020 | Union Omaha 2 at South Georgia Tormenta FC 2 | Final |
| September 12, 2020 | Richmond Kickers 1 at Chattanooga Red Wolves SC 2 | Final |
| September 12, 2020 | Orlando City B 0 at Fort Lauderdale CF 2 | Final |
| September 12, 2020 | North Texas SC 2 at FC Tucson 0 | Final |
| September 13, 2020 | Forward Madison FC 0 at Greenville Triumph SC 2 | Final |
| September 13, 2020 | Memphis 901 FC 2 at North Carolina FC 3 | Final |
| September 15, 2020 | Union Omaha 0 at Chattanooga Red Wolves SC 2 | Final |
| September 16, 2020 | New England Revolution II 0 at FC Tucson 1 | Final |
| September 19, 2020 | Charlotte Independence 1 at North Carolina FC 0 | Final |
| September 19, 2020 | Greenville Triumph SC 1 at Chattanooga Red Wolves SC 0 | Final |
| September 19, 2020 | Orlando City B at Richmond Kickers | Ppd. |
| September 19, 2020 | South Georgia Tormenta FC 2 at Fort Lauderdale CF 1 | Final |
| September 19, 2020 | New England Revolution II 2 at Union Omaha 0 | Final |
| September 19, 2020 | FC Tucson 1 at North Texas SC 1 | Final |
| September 23, 2020 | Fort Lauderdale CF 1 at Richmond Kickers 2 | Final |
| September 23, 2020 | North Carolina FC at Memphis 901 FC | Ppd. |
| September 23, 2020 | Forward Madison FC 1 at FC Tucson 2 | Final |
| September 25, 2020 | North Texas SC 0 at Greenville Triumph SC 1 | Final |
| September 26, 2020 | New England Revolution II 1 at Orlando City B 0 | Final |
| September 26, 2020 | Chattanooga Red Wolves SC 0 at Forward Madison FC 1 | Final |
| September 26, 2020 | North Carolina FC 2 at Birmingham Legion FC 1 | Final |
| September 26, 2020 | Richmond Kickers 1 at Union Omaha 0 | Final |
| September 26, 2020 | Fort Lauderdale CF 2 at FC Tucson 1 | Final |
| September 30, 2020 | North Carolina FC 3 at New York Red Bulls II 0 (Richmond, VA) | Final |
| September 30, 2020 | Union Omaha 3 at Fort Lauderdale CF 2 | Final |
| October 2, 2020 | Orlando City B 0 at Greenville Triumph SC 2 | Final |
| October 3, 2020 | Fort Lauderdale CF 0 at North Texas SC 3 | Final |
| October 3, 2020 | New England Revolution II 4 at Richmond Kickers 0 | Final |
| October 3, 2020 | Charlotte Independence 1 at North Carolina FC 0 | Final |
| October 3, 2020 | FC Tucson 0 at Forward Madison FC 0 | Final |
| October 3, 2020 | South Georgia Tormenta FC 0 at Union Omaha 3 | Final |
| October 4, 2020 | North Carolina FC at Loudoun United FC | Cancelled |
| October 7, 2020 | North Texas SC 3 at Chattanooga Red Wolves SC 1 | Final |
| October 7, 2020 | Orlando City B 1 at Richmond Kickers 2 | Final |
| October 7, 2020 | FC Tucson 1 at South Georgia Tormenta FC 0 | Final |
| October 9, 2020 | Fort Lauderdale CF 1 at New England Revolution II 4 | Final |
| October 10, 2020 | Chattanooga Red Wolves SC at Orlando City B | Ppd. |
| October 10, 2020 | Richmond Kickers 1 at North Texas SC 2 | Final |
| October 10, 2020 | Greenville Triumph SC 2 at FC Tucson 2 | Final |
| October 11, 2020 | Union Omaha 0 at Forward Madison FC 0 | Final |
| October 14, 2020 | Forward Madison FC 2 at Fort Lauderdale CF 2 | Final |
| October 16, 2020 | North Texas SC 1 at New England Revolution II 1 | Final |
| October 17, 2020 | Forward Madison FC 1 at Chattanooga Red Wolves SC 1 | Final |
| October 17, 2020 | South Georgia Tormenta FC 1 at Richmond Kickers 0 | Final |
| October 17, 2020 | Orlando City B 0 at FC Tucson 2 | Final |
| October 18, 2020 | Union Omaha 2 at Greenville Triumph SC 1 | Final |
| October 21, 2020 | Richmond Kickers 3 at Orlando City B 1 | Final |
| October 21, 2020 | New England Revolution II 0 at South Georgia Tormenta FC 4 | Final |
| October 21, 2020 | North Texas SC 1 at Forward Madison FC 0 | Final |
| October 24, 2020 | Greenville Triumph SC 4 at Orlando City B 1 | Final |
| October 24, 2020 | Chattanooga Red Wolves SC 2 at Richmond Kickers 1 | Final |
| October 24, 2020 | New England Revolution II 1 at Forward Madison FC 2 | Final |
| October 24, 2020 | Fort Lauderdale CF 0 at Union Omaha 1 | Final |
| October 24, 2020 | South Georgia Tormenta FC 1 at North Texas SC 2 | Final |
| October 30, 2020 | Union Omaha at Greenville Triumph SC (Championship) | Cancelled |
United Soccer League One Schedule
