| | | |
| March 27, 2020 | Chattanooga Red Wolves SC at Greenville Triumph SC | Ppd. |
| March 27, 2020 | Union Omaha at Fort Lauderdale CF | Ppd. |
| March 28, 2020 | Orlando City B at New England Revolution II | Ppd. |
| March 28, 2020 | Forward Madison FC at Richmond Kickers | Ppd. |
| March 28, 2020 | North Texas SC at South Georgia Tormenta FC | Ppd. |
| March 28, 2020 | Toronto FC II at FC Tucson | Ppd. |
| April 3, 2020 | Richmond Kickers at New England Revolution II | Ppd. |
| April 4, 2020 | South Georgia Tormenta FC at Greenville Triumph SC | Ppd. |
| April 4, 2020 | Orlando City B at North Texas SC | Ppd. |
| April 4, 2020 | Forward Madison FC at FC Tucson | Ppd. |
| April 11, 2020 | Chattanooga Red Wolves SC at Orlando City B | Ppd. |
| April 11, 2020 | Fort Lauderdale CF at South Georgia Tormenta FC | Ppd. |
| April 11, 2020 | Toronto FC II at Forward Madison FC | Ppd. |
| April 11, 2020 | North Texas SC at FC Tucson | Ppd. |
| April 12, 2020 | Greenville Triumph SC at New England Revolution II | Ppd. |
| April 17, 2020 | FC Tucson at Orlando City B | Ppd. |
| April 18, 2020 | Greenville Triumph SC at Richmond Kickers | Ppd. |
| April 18, 2020 | Toronto FC II at South Georgia Tormenta FC | Ppd. |
| April 18, 2020 | Union Omaha at Forward Madison FC | Ppd. |
| April 18, 2020 | Chattanooga Red Wolves SC at North Texas SC | Ppd. |
| April 19, 2020 | New England Revolution II at Fort Lauderdale CF | Ppd. |
| April 25, 2020 | Richmond Kickers at Chattanooga Red Wolves SC | Ppd. |
| April 25, 2020 | Toronto FC II at New England Revolution II | Ppd. |
| April 25, 2020 | Orlando City B at Union Omaha | Ppd. |
| April 26, 2020 | North Texas SC at Greenville Triumph SC | Ppd. |
| April 26, 2020 | Forward Madison FC at Fort Lauderdale CF | Ppd. |
| May 1, 2020 | Richmond Kickers at Toronto FC II | Ppd. |
| May 1, 2020 | Greenville Triumph SC at Orlando City B | Ppd. |
| May 2, 2020 | New England Revolution II at South Georgia Tormenta FC | Ppd. |
| May 2, 2020 | Chattanooga Red Wolves SC at Forward Madison FC | Ppd. |
| May 2, 2020 | Fort Lauderdale CF at FC Tucson | Ppd. |
| May 3, 2020 | Union Omaha at North Texas SC | Ppd. |
| May 6, 2020 | Greenville Triumph SC at Toronto FC II | Ppd. |
| May 9, 2020 | South Georgia Tormenta FC at Richmond Kickers | Ppd. |
| May 9, 2020 | FC Tucson at Chattanooga Red Wolves SC | Ppd. |
| May 9, 2020 | Union Omaha at Greenville Triumph SC | Ppd. |
| May 9, 2020 | Toronto FC II at Fort Lauderdale CF | Ppd. |
| May 9, 2020 | Forward Madison FC at North Texas SC | Ppd. |
| May 13, 2020 | Chattanooga Red Wolves SC at Fort Lauderdale CF | Ppd. |
| May 15, 2020 | Forward Madison FC at Toronto FC II | Ppd. |
| May 15, 2020 | Union Omaha at New England Revolution II | Ppd. |
| May 16, 2020 | Richmond Kickers at Orlando City B | Ppd. |
| May 16, 2020 | Chattanooga Red Wolves SC at South Georgia Tormenta FC | Ppd. |
| May 17, 2020 | FC Tucson at Greenville Triumph SC | Ppd. |
| May 22, 2020 | Fort Lauderdale CF at New England Revolution II | Ppd. |
| May 23, 2020 | Orlando City B at Chattanooga Red Wolves SC | Ppd. |
| May 23, 2020 | FC Tucson at Union Omaha | Ppd. |
| May 23, 2020 | South Georgia Tormenta FC at North Texas SC | Ppd. |
| May 27, 2020 | South Georgia Tormenta FC at Union Omaha | Ppd. |
| May 30, 2020 | North Texas SC at Richmond Kickers | Ppd. |
| May 30, 2020 | Toronto FC II at Chattanooga Red Wolves SC | Ppd. |
| May 30, 2020 | Fort Lauderdale CF at Greenville Triumph SC | Ppd. |
| May 30, 2020 | New England Revolution II at Forward Madison FC | Ppd. |
| May 30, 2020 | Orlando City B at FC Tucson | Ppd. |
| June 2, 2020 | South Georgia Tormenta FC at New England Revolution II | 7:00 p.m. |
| June 3, 2020 | Richmond Kickers at Toronto FC II | 4:00 p.m. |
| June 5, 2020 | New England Revolution II at Orlando City B | 7:00 p.m. |
| June 6, 2020 | Forward Madison FC at Richmond Kickers | 6:30 p.m. |
| June 6, 2020 | Union Omaha at Chattanooga Red Wolves SC | 7:00 p.m. |
| June 6, 2020 | FC Tucson at Fort Lauderdale CF | 7:30 p.m. |
| June 6, 2020 | Greenville Triumph SC at South Georgia Tormenta FC | 8:00 p.m. |
| June 6, 2020 | Toronto FC II at North Texas SC | 8:30 p.m. |
| June 11, 2020 | Chattanooga Red Wolves SC at Greenville Triumph SC | 7:00 p.m. |
| June 12, 2020 | Fort Lauderdale CF at Toronto FC II | 4:00 p.m. |
| June 12, 2020 | North Texas SC at New England Revolution II | 5:00 p.m. |
| June 12, 2020 | South Georgia Tormenta FC at Orlando City B | 7:00 p.m. |
| June 12, 2020 | Richmond Kickers at Union Omaha | 8:00 p.m. |
| June 13, 2020 | Forward Madison FC at FC Tucson | 10:30 p.m. |
| June 17, 2020 | Union Omaha at FC Tucson | 10:00 p.m. |
| June 20, 2020 | Greenville Triumph SC at Richmond Kickers | 6:30 p.m. |
| June 20, 2020 | New England Revolution II at Chattanooga Red Wolves SC | 7:00 p.m. |
| June 20, 2020 | Toronto FC II at Forward Madison FC | 8:00 p.m. |
| June 20, 2020 | FC Tucson at South Georgia Tormenta FC | 8:00 p.m. |
| June 21, 2020 | Orlando City B at Fort Lauderdale CF | 7:30 p.m. |
| June 21, 2020 | Union Omaha at North Texas SC | 8:00 p.m. |
| June 26, 2020 | South Georgia Tormenta FC at Toronto FC II | 4:00 p.m. |
| June 27, 2020 | Orlando City B at Richmond Kickers | 6:30 p.m. |
| June 27, 2020 | Fort Lauderdale CF at Forward Madison FC | 8:00 p.m. |
| June 27, 2020 | New England Revolution II at Union Omaha | 8:00 p.m. |
| June 27, 2020 | Greenville Triumph SC at North Texas SC | 8:30 p.m. |
| June 27, 2020 | Chattanooga Red Wolves SC at FC Tucson | 10:30 p.m. |
| July 1, 2020 | Chattanooga Red Wolves SC at Toronto FC II | 4:00 p.m. |
| July 2, 2020 | Forward Madison FC at Greenville Triumph SC | 7:00 p.m. |
| July 2, 2020 | Union Omaha at Orlando City B | 7:00 p.m. |
| July 2, 2020 | Richmond Kickers at Fort Lauderdale CF | 7:30 p.m. |
| July 3, 2020 | New England Revolution II at FC Tucson | 10:00 p.m. |
| July 5, 2020 | North Texas SC at South Georgia Tormenta FC | 8:00 p.m. |
| July 8, 2020 | Toronto FC II at New England Revolution II | 1:00 p.m. |
| July 11, 2020 | FC Tucson at Richmond Kickers | 6:30 p.m. |
| July 11, 2020 | South Georgia Tormenta FC at Chattanooga Red Wolves SC | 7:00 p.m. |
| July 11, 2020 | Greenville Triumph SC at Fort Lauderdale CF | 7:30 p.m. |
| July 11, 2020 | Union Omaha at Forward Madison FC | 8:00 p.m. |
| July 12, 2020 | New England Revolution II at North Texas SC | 8:00 p.m. |
| July 15, 2020 | Orlando City B at Forward Madison FC | 8:00 p.m. |
| July 17, 2020 | Fort Lauderdale CF at Toronto FC II | 4:00 p.m. |
| July 17, 2020 | Richmond Kickers at Union Omaha | 8:00 p.m. |
| July 18, 2020 | North Texas SC at Chattanooga Red Wolves SC | 7:00 p.m. |
| July 18, 2020 | Orlando City B at Greenville Triumph SC | 7:00 p.m. |
| July 18, 2020 | South Georgia Tormenta FC at FC Tucson | 10:30 p.m. |
| July 22, 2020 | Forward Madison FC at New England Revolution II | 5:00 p.m. |
| July 24, 2020 | Union Omaha at Toronto FC II | 4:00 p.m. |
| July 24, 2020 | Fort Lauderdale CF at Orlando City B | 7:00 p.m. |
| July 25, 2020 | South Georgia Tormenta FC at Richmond Kickers | 6:30 p.m. |
| July 25, 2020 | North Texas SC at Forward Madison FC | 8:00 p.m. |
| July 25, 2020 | Greenville Triumph SC at FC Tucson | 10:30 p.m. |
| July 26, 2020 | Chattanooga Red Wolves SC at New England Revolution II | 2:00 p.m. |
| July 31, 2020 | Orlando City B at Toronto FC II | 4:00 p.m. |
| July 31, 2020 | Richmond Kickers at Greenville Triumph SC | 7:00 p.m. |
| August 1, 2020 | Chattanooga Red Wolves SC at Fort Lauderdale CF | 7:30 p.m. |
| August 1, 2020 | Forward Madison FC at South Georgia Tormenta FC | 8:00 p.m. |
| August 1, 2020 | New England Revolution II at Union Omaha | 8:00 p.m. |
| August 1, 2020 | FC Tucson at North Texas SC | 8:30 p.m. |
| August 6, 2020 | Fort Lauderdale CF at Forward Madison FC | 8:00 p.m. |
| August 7, 2020 | North Texas SC at Toronto FC II | 4:00 p.m. |
| August 8, 2020 | New England Revolution II at Richmond Kickers | 6:30 p.m. |
| August 8, 2020 | Greenville Triumph SC at Chattanooga Red Wolves SC | 7:00 p.m. |
| August 8, 2020 | Union Omaha at FC Tucson | 10:30 p.m. |
| August 9, 2020 | Orlando City B at South Georgia Tormenta FC | 8:00 p.m. |
| August 14, 2020 | New England Revolution II at Toronto FC II | 4:00 p.m. |
| August 14, 2020 | FC Tucson at Orlando City B | 7:00 p.m. |
| August 14, 2020 | Forward Madison FC at Union Omaha | 8:00 p.m. |
| August 15, 2020 | Chattanooga Red Wolves SC at Richmond Kickers | 6:30 p.m. |
| August 15, 2020 | North Texas SC at Greenville Triumph SC | 7:00 p.m. |
| August 15, 2020 | South Georgia Tormenta FC at Fort Lauderdale CF | 7:30 p.m. |
| August 19, 2020 | Union Omaha at South Georgia Tormenta FC | 8:00 p.m. |
| August 19, 2020 | Forward Madison FC at North Texas SC | 8:30 p.m. |
| August 21, 2020 | FC Tucson at New England Revolution II | 5:00 p.m. |
| August 22, 2020 | Orlando City B at Chattanooga Red Wolves SC | 7:00 p.m. |
| August 22, 2020 | Toronto FC II at Greenville Triumph SC | 7:00 p.m. |
| August 22, 2020 | Richmond Kickers at Forward Madison FC | 8:00 p.m. |
| August 22, 2020 | Fort Lauderdale CF at North Texas SC | 8:30 p.m. |
| August 25, 2020 | Toronto FC II at Union Omaha | 8:00 p.m. |
| August 28, 2020 | FC Tucson at Toronto FC II | 4:00 p.m. |
| August 28, 2020 | New England Revolution II at Orlando City B | 7:00 p.m. |
| August 29, 2020 | Fort Lauderdale CF at Richmond Kickers | 6:30 p.m. |
| August 29, 2020 | South Georgia Tormenta FC at Chattanooga Red Wolves SC | 7:00 p.m. |
| August 29, 2020 | Greenville Triumph SC at Forward Madison FC | 8:00 p.m. |
| August 29, 2020 | North Texas SC at Union Omaha | 8:00 p.m. |
| September 3, 2020 | North Texas SC at Orlando City B | 7:00 p.m. |
| September 4, 2020 | Richmond Kickers at FC Tucson | 10:30 p.m. |
| September 5, 2020 | Toronto FC II at Fort Lauderdale CF | 7:30 p.m. |
| September 5, 2020 | Greenville Triumph SC at South Georgia Tormenta FC | 8:00 p.m. |
| September 6, 2020 | Chattanooga Red Wolves SC at Union Omaha | 8:00 p.m. |
| September 10, 2020 | FC Tucson at Forward Madison FC | 8:00 p.m. |
| September 11, 2020 | North Texas SC at Toronto FC II | 4:00 p.m. |
| September 11, 2020 | Richmond Kickers at New England Revolution II | 5:00 p.m. |
| September 11, 2020 | Orlando City B at South Georgia Tormenta FC | 8:00 p.m. |
| September 12, 2020 | Fort Lauderdale CF at Chattanooga Red Wolves SC | 7:00 p.m. |
| September 12, 2020 | Greenville Triumph SC at Union Omaha | 7:00 p.m. |
| September 15, 2020 | Forward Madison FC at Orlando City B | 7:00 p.m. |
| September 15, 2020 | Richmond Kickers at North Texas SC | 8:30 p.m. |
| September 18, 2020 | Greenville Triumph SC at Orlando City B | 7:00 p.m. |
| September 19, 2020 | Union Omaha at Richmond Kickers | 6:30 p.m. |
| September 19, 2020 | New England Revolution II at Chattanooga Red Wolves SC | 7:00 p.m. |
| September 20, 2020 | FC Tucson at Fort Lauderdale CF | 7:30 p.m. |
| September 23, 2020 | Richmond Kickers at South Georgia Tormenta FC | 8:00 p.m. |
| September 23, 2020 | Fort Lauderdale CF at Union Omaha | 8:00 p.m. |
| September 24, 2020 | Toronto FC II at Orlando City B | 7:00 p.m. |
| September 25, 2020 | New England Revolution II at Greenville Triumph SC | 7:00 p.m. |
| September 26, 2020 | North Texas SC at Fort Lauderdale CF | 7:30 p.m. |
| September 26, 2020 | South Georgia Tormenta FC at Forward Madison FC | 8:00 p.m. |
| September 26, 2020 | Chattanooga Red Wolves SC at FC Tucson | 10:30 p.m. |
| October 2, 2020 | Toronto FC II at Richmond Kickers | 6:30 p.m. |
| October 2, 2020 | Fort Lauderdale CF at Greenville Triumph SC | 7:00 p.m. |
| October 3, 2020 | South Georgia Tormenta FC at New England Revolution II | 1:00 p.m. |
| October 3, 2020 | Forward Madison FC at Chattanooga Red Wolves SC | 7:00 p.m. |
| October 3, 2020 | Orlando City B at Union Omaha | 8:00 p.m. |
| October 3, 2020 | FC Tucson at North Texas SC | 8:30 p.m. |
United Soccer League One Schedule
