| | | |
| February 22, 2025 | T | Toronto FC 2 at D.C. United 2 | Final |
| March 1, 2025 | T | D.C. United 2 at Chicago Fire FC 2 | Final |
| March 8, 2025 | W | Sporting Kansas City 1 at D.C. United 2 | Final |
| March 15, 2025 | T | Club de Foot Montreal 0 at D.C. United 0 | Final |
| March 22, 2025 | L | D.C. United 1 at Orlando City SC 4 | Final |
| March 29, 2025 | L | Columbus Crew SC 2 at D.C. United 1 | Final |
| April 6, 2025 | L | D.C. United 1 at San Jose Earthquakes 6 | Final |
| April 12, 2025 | L | FC Cincinnati 1 at D.C. United 0 | Final |
| April 19, 2025 | W | D.C. United 2 at New York Red Bulls 1 | Final |
| April 26, 2025 | L | D.C. United 0 at Philadelphia Union 3 | Final |
| May 3, 2025 | | Colorado Rapids at D.C. United | 7:30 p.m. |
| May 10, 2025 | | D.C. United at Toronto FC | 4:30 p.m. |
| May 14, 2025 | | New York City FC at D.C. United | 7:30 p.m. |
| May 17, 2025 | | D.C. United at Nashville SC | 8:30 p.m. |
| May 24, 2025 | | New York Red Bulls at D.C. United | 7:30 p.m. |
| May 28, 2025 | | New England Revolution at D.C. United | 7:30 p.m. |
| May 31, 2025 | | D.C. United at FC Cincinnati | 7:30 p.m. |
| June 7, 2025 | | Chicago Fire FC at D.C. United | 7:30 p.m. |
| June 14, 2025 | | D.C. United at Real Salt Lake | 9:30 p.m. |
| June 28, 2025 | | Nashville SC at D.C. United | 7:30 p.m. |
| July 5, 2025 | | Atlanta United FC at D.C. United | 7:30 p.m. |
| July 12, 2025 | | D.C. United at LA Galaxy | 10:30 p.m. |
| July 16, 2025 | | D.C. United at Charlotte FC | 7:30 p.m. |
| July 19, 2025 | | D.C. United at Columbus Crew SC | 7:30 p.m. |
| July 26, 2025 | | Austin FC at D.C. United | 7:30 p.m. |
| August 9, 2025 | | D.C. United at New England Revolution | 7:30 p.m. |
| August 16, 2025 | | D.C. United at Club de Foot Montreal | 7:30 p.m. |
| August 23, 2025 | | Inter Miami CF at D.C. United | 7:30 p.m. |
| August 30, 2025 | | D.C. United at New York City FC | 7:30 p.m. |
| September 13, 2025 | | Orlando City SC at D.C. United | 7:30 p.m. |
| September 20, 2025 | | D.C. United at Inter Miami CF | 7:30 p.m. |
| September 27, 2025 | | Philadelphia Union at D.C. United | 7:30 p.m. |
| October 4, 2025 | | Charlotte FC at D.C. United | 2:30 p.m. |
| October 18, 2025 | | D.C. United at Atlanta United FC | 6:00 p.m. |
D.C. United Schedule
