| | | |
| February 23, 2025 | W | San Diego FC 2 at LA Galaxy 0 | Final |
| March 1, 2025 | T | St. Louis City SC 0 at San Diego FC 0 | Final |
| March 8, 2025 | W | San Diego FC 3 at Real Salt Lake 1 | Final |
| March 15, 2025 | T | Columbus Crew SC 1 at San Diego FC 1 | Final |
| March 23, 2025 | L | San Diego FC 1 at Austin FC 2 | Final |
| March 29, 2025 | W | Los Angeles FC 2 at San Diego FC 3 | Final |
| April 5, 2025 | W | Seattle Sounders FC 0 at San Diego FC 3 | Final |
| April 12, 2025 | L | San Diego FC 2 at Colorado Rapids 3 | Final |
| April 19, 2025 | L | San Diego FC 0 at Charlotte FC 3 | Final |
| April 26, 2025 | L | Real Salt Lake 3 at San Diego FC 1 | Final |
| May 3, 2025 | W | FC Dallas 0 at San Diego FC 5 | Final |
| May 10, 2025 | | San Diego FC at St. Louis City SC | 8:30 p.m. |
| May 14, 2025 | | Colorado Rapids at San Diego FC | 10:30 p.m. |
| May 17, 2025 | | Sporting Kansas City at San Diego FC | 10:30 p.m. |
| May 24, 2025 | | LA Galaxy at San Diego FC | 4:30 p.m. |
| May 28, 2025 | | San Diego FC at Seattle Sounders FC | 8:30 p.m. |
| May 31, 2025 | | Austin FC at San Diego FC | 10:30 p.m. |
| June 14, 2025 | | San Diego FC at Minnesota United FC | 8:30 p.m. |
| June 25, 2025 | | San Diego FC at Vancouver Whitecaps FC | 10:30 p.m. |
| June 28, 2025 | | San Diego FC at FC Dallas | 8:30 p.m. |
| July 5, 2025 | | Houston Dynamo FC at San Diego FC | 10:30 p.m. |
| July 12, 2025 | | San Diego FC at Chicago Fire FC | 8:30 p.m. |
| July 16, 2025 | | Toronto FC at San Diego FC | 10:30 p.m. |
| July 19, 2025 | | Vancouver Whitecaps FC at San Diego FC | 10:30 p.m. |
| July 25, 2025 | | Nashville SC at San Diego FC | 10:30 p.m. |
| August 9, 2025 | | San Diego FC at Sporting Kansas City | 8:30 p.m. |
| August 17, 2025 | | San Diego FC at San Jose Earthquakes | 7:00 p.m. |
| August 23, 2025 | | Portland Timbers at San Diego FC | 10:30 p.m. |
| August 31, 2025 | | San Diego FC at Los Angeles FC | 10:30 p.m. |
| September 13, 2025 | | Minnesota United FC at San Diego FC | 10:30 p.m. |
| September 20, 2025 | | San Diego FC at Atlanta United FC | 4:30 p.m. |
| September 27, 2025 | | San Jose Earthquakes at San Diego FC | 10:30 p.m. |
| October 4, 2025 | | San Diego FC at Houston Dynamo FC | 8:30 p.m. |
| October 18, 2025 | | San Diego FC at Portland Timbers | 9:00 p.m. |
San Diego FC Schedule
