| | | |
| February 21, 2026 | T | Charlotte FC 1 at St. Louis City SC 1 | Final |
| March 1, 2026 | L | St. Louis City SC 0 at San Diego FC 2 | Final |
| March 7, 2026 | L | Seattle Sounders FC 1 at St. Louis City SC 0 | Final |
| March 14, 2026 | L | St. Louis City SC 0 at Los Angeles FC 2 | Final |
| March 21, 2026 | W | New England Revolution 1 at St. Louis City SC 3 | Final |
| April 4, 2026 | | St. Louis City SC at New York City FC | 6:30 p.m. |
| April 11, 2026 | | St. Louis City SC at FC Dallas | 7:30 p.m. |
| April 18, 2026 | | St. Louis City SC at Seattle Sounders FC | 8:30 p.m. |
| April 25, 2026 | | San Jose Earthquakes at St. Louis City SC | 7:30 p.m. |
| May 3, 2026 | | St. Louis City SC at Austin FC | 4:30 p.m. |
| May 9, 2026 | | St. Louis City SC at Colorado Rapids | 8:30 p.m. |
| May 13, 2026 | | Los Angeles FC at St. Louis City SC | 7:30 p.m. |
| May 16, 2026 | | St. Louis City SC at D.C. United | 6:30 p.m. |
| May 23, 2026 | | Austin FC at St. Louis City SC | 1:30 p.m. |
| July 16, 2026 | | Sporting Kansas City at St. Louis City SC | 7:30 p.m. |
| July 22, 2026 | | St. Louis City SC at LA Galaxy | 9:30 p.m. |
| July 25, 2026 | | Colorado Rapids at St. Louis City SC | 7:30 p.m. |
| August 1, 2026 | | Real Salt Lake at St. Louis City SC | 7:30 p.m. |
| August 15, 2026 | | St. Louis City SC at San Jose Earthquakes | 9:30 p.m. |
| August 19, 2026 | | St. Louis City SC at Sporting Kansas City | 7:00 p.m. |
| August 22, 2026 | | Houston Dynamo FC at St. Louis City SC | 7:30 p.m. |
| August 30, 2026 | | FC Dallas at St. Louis City SC | 6:00 p.m. |
| September 5, 2026 | | St. Louis City SC at Vancouver Whitecaps FC | 9:30 p.m. |
| September 9, 2026 | | St. Louis City SC at Portland Timbers | 9:30 p.m. |
| September 12, 2026 | | Minnesota United FC at St. Louis City SC | 7:30 p.m. |
| September 19, 2026 | | Toronto FC at St. Louis City SC | 7:30 p.m. |
| September 26, 2026 | | St. Louis City SC at Red Bull New York | 6:30 p.m. |
| October 11, 2026 | | LA Galaxy at St. Louis City SC | 6:00 p.m. |
| October 14, 2026 | | Vancouver Whitecaps FC at St. Louis City SC | 7:30 p.m. |
| October 17, 2026 | | St. Louis City SC at Minnesota United FC | 3:30 p.m. |
| October 24, 2026 | | St. Louis City SC at Real Salt Lake | 8:30 p.m. |
| October 28, 2026 | | Portland Timbers at St. Louis City SC | 7:30 p.m. |
| October 31, 2026 | | San Diego FC at St. Louis City SC | 1:00 p.m. |
| November 7, 2026 | | St. Louis City SC at Houston Dynamo FC | 7:00 p.m. |
St. Louis City SC Schedule
