| | | |
| February 22, 2025 | L | FC Dallas 2 at Houston Dynamo FC 1 | Final |
| March 2, 2025 | L | Inter Miami CF 4 at Houston Dynamo FC 1 | Final |
| March 8, 2025 | T | Houston Dynamo FC 0 at Columbus Crew SC 0 | Final |
| March 15, 2025 | L | Real Salt Lake 2 at Houston Dynamo FC 1 | Final |
| March 22, 2025 | T | Houston Dynamo FC 0 at Seattle Sounders FC 0 | Final |
| March 30, 2025 | L | Houston Dynamo FC 1 at Portland Timbers 3 | Final |
| April 5, 2025 | W | Los Angeles FC 0 at Houston Dynamo FC 1 | Final |
| April 12, 2025 | T | Houston Dynamo FC 1 at LA Galaxy 1 | Final |
| April 19, 2025 | T | Colorado Rapids 2 at Houston Dynamo FC 2 | Final |
| April 26, 2025 | W | Austin FC 0 at Houston Dynamo FC 2 | Final |
| May 3, 2025 | L | Houston Dynamo FC 0 at Los Angeles FC 2 | Final |
| May 10, 2025 | L | Seattle Sounders FC 3 at Houston Dynamo FC 1 | Final |
| May 14, 2025 | W | Minnesota United FC 0 at Houston Dynamo FC 2 | Final |
| May 17, 2025 | W | Houston Dynamo FC 2 at FC Dallas 0 | Final |
| May 24, 2025 | T | Houston Dynamo FC 3 at San Jose Earthquakes 3 | Final |
| May 28, 2025 | W | Houston Dynamo FC 3 at New York City FC 0 | Final |
| May 31, 2025 | L | Sporting Kansas City 3 at Houston Dynamo FC 1 | Final |
| June 14, 2025 | L | Club de Foot Montreal 3 at Houston Dynamo FC 1 | Final |
| June 25, 2025 | L | Houston Dynamo FC 1 at Minnesota United FC 3 | Final |
| June 28, 2025 | W | St. Louis City SC 0 at Houston Dynamo FC 1 | Final |
| July 5, 2025 | W | Houston Dynamo FC 4 at San Diego FC 3 | Final |
| July 12, 2025 | L | Houston Dynamo FC 0 at Real Salt Lake 1 | Final |
| July 16, 2025 | L | Vancouver Whitecaps FC 3 at Houston Dynamo FC 0 | Final |
| July 19, 2025 | T | Philadelphia Union 1 at Houston Dynamo FC 1 | Final |
| July 25, 2025 | | LA Galaxy at Houston Dynamo FC | 8:30 p.m. |
| August 9, 2025 | | Houston Dynamo FC at Austin FC | 8:30 p.m. |
| August 17, 2025 | | Houston Dynamo FC at Vancouver Whitecaps FC | 9:00 p.m. |
| August 23, 2025 | | San Jose Earthquakes at Houston Dynamo FC | 8:30 p.m. |
| August 30, 2025 | | Houston Dynamo FC at St. Louis City SC | 8:30 p.m. |
| September 13, 2025 | | Houston Dynamo FC at Colorado Rapids | 9:30 p.m. |
| September 20, 2025 | | Portland Timbers at Houston Dynamo FC | 8:30 p.m. |
| September 27, 2025 | | Houston Dynamo FC at Nashville SC | 8:30 p.m. |
| October 4, 2025 | | San Diego FC at Houston Dynamo FC | 8:30 p.m. |
| October 18, 2025 | | Houston Dynamo FC at Sporting Kansas City | 9:00 p.m. |
Houston Dynamo FC Schedule
