| | | |
| February 22, 2025 | L | Philadelphia Union 4 at Orlando City SC 2 | Final |
| March 1, 2025 | W | Toronto FC 2 at Orlando City SC 4 | Final |
| March 8, 2025 | L | Orlando City SC 1 at New York City FC 2 | Final |
| March 15, 2025 | T | Orlando City SC 2 at New York Red Bulls 2 | Final |
| March 22, 2025 | W | D.C. United 1 at Orlando City SC 4 | Final |
| March 29, 2025 | W | Orlando City SC 2 at LA Galaxy 1 | Final |
| April 5, 2025 | T | Orlando City SC 0 at Philadelphia Union 0 | Final |
| April 12, 2025 | T | New York Red Bulls 0 at Orlando City SC 0 | Final |
| April 19, 2025 | T | Orlando City SC 0 at Club de Foot Montreal 0 | Final |
| April 26, 2025 | W | Atlanta United FC 0 at Orlando City SC 3 | Final |
| May 3, 2025 | T | Orlando City SC 0 at Chicago Fire FC 0 | Final |
| May 10, 2025 | T | New England Revolution 3 at Orlando City SC 3 | Final |
| May 14, 2025 | W | Charlotte FC 1 at Orlando City SC 3 | Final |
| May 18, 2025 | W | Orlando City SC 3 at Inter Miami CF 0 | Final |
| May 24, 2025 | W | Portland Timbers 0 at Orlando City SC 1 | Final |
| May 28, 2025 | L | Orlando City SC 2 at Atlanta United FC 3 | Final |
| May 31, 2025 | L | Chicago Fire FC 3 at Orlando City SC 1 | Final |
| June 14, 2025 | W | Orlando City SC 1 at Colorado Rapids 0 | Final |
| June 25, 2025 | | Orlando City SC at St. Louis City SC | 8:30 p.m. |
| June 28, 2025 | | FC Cincinnati at Orlando City SC | 7:30 p.m. |
| July 5, 2025 | | Orlando City SC at Charlotte FC | 7:00 p.m. |
| July 12, 2025 | | Club de Foot Montreal at Orlando City SC | 7:30 p.m. |
| July 16, 2025 | | New York City FC at Orlando City SC | 7:30 p.m. |
| July 19, 2025 | | Orlando City SC at New England Revolution | 7:30 p.m. |
| July 25, 2025 | | Orlando City SC at Columbus Crew SC | 7:30 p.m. |
| August 10, 2025 | | Inter Miami CF at Orlando City SC | 8:00 p.m. |
| August 16, 2025 | | Sporting Kansas City at Orlando City SC | 7:30 p.m. |
| August 23, 2025 | | Orlando City SC at Nashville SC | 8:30 p.m. |
| August 30, 2025 | | Vancouver Whitecaps FC at Orlando City SC | 7:30 p.m. |
| September 13, 2025 | | Orlando City SC at D.C. United | 7:30 p.m. |
| September 20, 2025 | | Nashville SC at Orlando City SC | 7:30 p.m. |
| September 27, 2025 | | Orlando City SC at FC Cincinnati | 2:30 p.m. |
| October 4, 2025 | | Columbus Crew SC at Orlando City SC | 7:30 p.m. |
| October 18, 2025 | | Orlando City SC at Toronto FC | 6:00 p.m. |
Orlando City SC Schedule
