| | | |
| February 22, 2025 | W | New York Red Bulls 0 at FC Cincinnati 1 | Final |
| March 1, 2025 | L | FC Cincinnati 1 at Philadelphia Union 4 | Final |
| March 8, 2025 | W | Toronto FC 0 at FC Cincinnati 2 | Final |
| March 15, 2025 | L | FC Cincinnati 0 at Charlotte FC 2 | Final |
| March 22, 2025 | T | Atlanta United FC 2 at FC Cincinnati 2 | Final |
| March 29, 2025 | W | FC Cincinnati 2 at Nashville SC 1 | Final |
| April 5, 2025 | W | New England Revolution 0 at FC Cincinnati 1 | Final |
| April 12, 2025 | W | FC Cincinnati 1 at D.C. United 0 | Final |
| April 19, 2025 | W | FC Cincinnati 3 at Chicago Fire FC 2 | Final |
| April 26, 2025 | W | Sporting Kansas City 1 at FC Cincinnati 2 | Final |
| May 4, 2025 | L | FC Cincinnati 0 at New York City FC 1 | Final |
| May 10, 2025 | W | Austin FC 1 at FC Cincinnati 2 | Final |
| May 14, 2025 | W | FC Cincinnati 1 at Toronto FC 0 | Final |
| May 17, 2025 | T | FC Cincinnati 1 at Columbus Crew SC 1 | Final |
| May 25, 2025 | L | FC Cincinnati 2 at Atlanta United FC 4 | Final |
| May 28, 2025 | T | FC Dallas 3 at FC Cincinnati 3 | Final |
| May 31, 2025 | L | D.C. United 2 at FC Cincinnati 1 | Final |
| June 14, 2025 | W | FC Cincinnati 1 at New England Revolution 0 | Final |
| June 25, 2025 | W | FC Cincinnati 3 at Club de Foot Montreal 1 | Final |
| June 28, 2025 | W | FC Cincinnati 2 at Orlando City SC 1 | Final |
| July 5, 2025 | W | Chicago Fire FC 1 at FC Cincinnati 2 | Final |
| July 12, 2025 | L | Columbus Crew SC 4 at FC Cincinnati 2 | Final |
| July 16, 2025 | W | Inter Miami CF 0 at FC Cincinnati 3 | Final |
| July 19, 2025 | W | FC Cincinnati 1 at Real Salt Lake 0 | Final |
| July 26, 2025 | T | FC Cincinnati 0 at Inter Miami CF 0 | Final |
| August 10, 2025 | L | Charlotte FC 1 at FC Cincinnati 0 | Final |
| August 16, 2025 | W | FC Cincinnati 3 at Portland Timbers 2 | Final |
| August 23, 2025 | L | New York City FC 1 at FC Cincinnati 0 | Final |
| August 30, 2025 | L | Philadelphia Union 1 at FC Cincinnati 0 | Final |
| September 13, 2025 | W | Nashville SC 1 at FC Cincinnati 2 | Final |
| September 20, 2025 | W | FC Cincinnati 3 at LA Galaxy 2 | Final |
| September 28, 2025 | T | Orlando City SC 1 at FC Cincinnati 1 | Final |
| October 4, 2025 | W | FC Cincinnati 1 at New York Red Bulls 0 | Final |
| October 18, 2025 | W | Club de Foot Montreal 0 at FC Cincinnati 3 | Final |
| October 27, 2025 | W | Columbus Crew SC 0 at FC Cincinnati 1 (Playoff Gm. 1) | Final |
| November 2, 2025 | L | FC Cincinnati 0 at Columbus Crew SC 4 (Playoff Gm. 2) | Final |
| November 8, 2025 | | Columbus Crew SC at FC Cincinnati (Playoff Gm. 3) | 6:00 p.m. |
FC Cincinnati Schedule
