| | | |
| February 22, 2025 | T | Charlotte FC 2 at Seattle Sounders FC 2 | Final |
| March 1, 2025 | W | Atlanta United FC 0 at Charlotte FC 2 | Final |
| March 9, 2025 | L | Charlotte FC 0 at Inter Miami CF 1 | Final |
| March 15, 2025 | W | FC Cincinnati 0 at Charlotte FC 2 | Final |
| March 22, 2025 | W | San Jose Earthquakes 1 at Charlotte FC 4 | Final |
| March 29, 2025 | L | Charlotte FC 0 at Colorado Rapids 2 | Final |
| April 5, 2025 | W | Nashville SC 1 at Charlotte FC 2 | Final |
| April 12, 2025 | W | Charlotte FC 1 at Club de Foot Montreal 0 | Final |
| April 19, 2025 | W | San Diego FC 0 at Charlotte FC 3 | Final |
| April 26, 2025 | L | New England Revolution 1 at Charlotte FC 0 | Final |
| May 3, 2025 | L | Charlotte FC 2 at Columbus Crew SC 4 | Final |
| May 10, 2025 | L | Charlotte FC 1 at Nashville SC 2 | Final |
| May 14, 2025 | L | Charlotte FC 1 at Orlando City SC 3 | Final |
| May 17, 2025 | L | Chicago Fire FC 4 at Charlotte FC 1 | Final |
| May 24, 2025 | W | Columbus Crew SC 2 at Charlotte FC 3 | Final |
| May 28, 2025 | L | Charlotte FC 2 at New York Red Bulls 4 | Final |
| May 31, 2025 | W | Charlotte FC 2 at Toronto FC 0 | Final |
| June 14, 2025 | L | Charlotte FC 1 at Philadelphia Union 2 | Final |
| June 25, 2025 | L | Charlotte FC 1 at Sporting Kansas City 2 | Final |
| June 28, 2025 | L | Charlotte FC 2 at Chicago Fire FC 3 | Final |
| July 5, 2025 | T | Orlando City SC 2 at Charlotte FC 2 | Final |
| July 12, 2025 | W | New York City FC 0 at Charlotte FC 2 | Final |
| July 16, 2025 | W | D.C. United 1 at Charlotte FC 2 | Final |
| July 19, 2025 | W | Charlotte FC 3 at Atlanta United FC 2 | Final |
| July 26, 2025 | W | Toronto FC 0 at Charlotte FC 2 | Final |
| August 10, 2025 | | Charlotte FC at FC Cincinnati | 6:00 p.m. |
| August 16, 2025 | | Real Salt Lake at Charlotte FC | 7:30 p.m. |
| August 24, 2025 | | New York Red Bulls at Charlotte FC | 7:00 p.m. |
| August 30, 2025 | | Charlotte FC at New England Revolution | 7:30 p.m. |
| September 13, 2025 | | Inter Miami CF at Charlotte FC | 7:30 p.m. |
| September 19, 2025 | | Charlotte FC at New York City FC | 7:30 p.m. |
| September 27, 2025 | | Club de Foot Montreal at Charlotte FC | 7:30 p.m. |
| October 4, 2025 | | Charlotte FC at D.C. United | 2:30 p.m. |
| October 18, 2025 | | Philadelphia Union at Charlotte FC | 6:00 p.m. |
Charlotte FC Schedule
