| | | |
| February 22, 2025 | W | New York Red Bulls 0 at FC Cincinnati 1 | Final |
| March 1, 2025 | L | FC Cincinnati 1 at Philadelphia Union 4 | Final |
| March 8, 2025 | W | Toronto FC 0 at FC Cincinnati 2 | Final |
| March 15, 2025 | L | FC Cincinnati 0 at Charlotte FC 2 | Final |
| March 22, 2025 | T | Atlanta United FC 2 at FC Cincinnati 2 | Final |
| March 29, 2025 | W | FC Cincinnati 2 at Nashville SC 1 | Final |
| April 5, 2025 | W | New England Revolution 0 at FC Cincinnati 1 | Final |
| April 12, 2025 | W | FC Cincinnati 1 at D.C. United 0 | Final |
| April 19, 2025 | W | FC Cincinnati 3 at Chicago Fire FC 2 | Final |
| April 26, 2025 | W | Sporting Kansas City 1 at FC Cincinnati 2 | Final |
| May 4, 2025 | L | FC Cincinnati 0 at New York City FC 1 | Final |
| May 10, 2025 | W | Austin FC 1 at FC Cincinnati 2 | Final |
| May 14, 2025 | W | FC Cincinnati 1 at Toronto FC 0 | Final |
| May 17, 2025 | T | FC Cincinnati 1 at Columbus Crew SC 1 | Final |
| May 25, 2025 | L | FC Cincinnati 2 at Atlanta United FC 4 | Final |
| May 28, 2025 | T | FC Dallas 3 at FC Cincinnati 3 | Final |
| May 31, 2025 | L | D.C. United 2 at FC Cincinnati 1 | Final |
| June 14, 2025 | W | FC Cincinnati 1 at New England Revolution 0 | Final |
| June 25, 2025 | W | FC Cincinnati 3 at Club de Foot Montreal 1 | Final |
| June 28, 2025 | W | FC Cincinnati 2 at Orlando City SC 1 | Final |
| July 5, 2025 | W | Chicago Fire FC 1 at FC Cincinnati 2 | Final |
| July 12, 2025 | L | Columbus Crew SC 4 at FC Cincinnati 2 | Final |
| July 16, 2025 | W | Inter Miami CF 0 at FC Cincinnati 3 | Final |
| July 19, 2025 | W | FC Cincinnati 1 at Real Salt Lake 0 | Final |
| July 26, 2025 | T | FC Cincinnati 0 at Inter Miami CF 0 | Final |
| August 10, 2025 | | Charlotte FC at FC Cincinnati | 6:00 p.m. |
| August 16, 2025 | | FC Cincinnati at Portland Timbers | 10:30 p.m. |
| August 23, 2025 | | New York City FC at FC Cincinnati | 7:30 p.m. |
| August 30, 2025 | | Philadelphia Union at FC Cincinnati | 7:30 p.m. |
| September 13, 2025 | | Nashville SC at FC Cincinnati | 7:30 p.m. |
| September 20, 2025 | | FC Cincinnati at LA Galaxy | 10:30 p.m. |
| September 27, 2025 | | Orlando City SC at FC Cincinnati | 2:30 p.m. |
| October 4, 2025 | | FC Cincinnati at New York Red Bulls | 7:30 p.m. |
| October 18, 2025 | | Club de Foot Montreal at FC Cincinnati | 6:00 p.m. |
FC Cincinnati Schedule
