| | | |
| March 7, 2020 | W | Miami FC 1 at Saint Louis FC 4 | Final |
| March 14, 2020 | | Saint Louis FC at Memphis 901 FC | Ppd. |
| March 21, 2020 | | Indy Eleven at Saint Louis FC | Ppd. |
| March 28, 2020 | | Hartford Athletic at Saint Louis FC | Ppd. |
| April 11, 2020 | | Saint Louis FC at New York Red Bulls II | Ppd. |
| April 18, 2020 | | Charleston Battery at Saint Louis FC | Ppd. |
| April 25, 2020 | | Saint Louis FC at Pittsburgh Riverhounds SC | Ppd. |
| May 2, 2020 | | Tampa Bay Rowdies at Saint Louis FC | Ppd. |
| May 9, 2020 | | Sporting Kansas City II at Saint Louis FC | Ppd. |
| May 16, 2020 | | Saint Louis FC at Birmingham Legion FC | Ppd. |
| May 24, 2020 | | Saint Louis FC at Philadelphia Union II | Ppd. |
| May 30, 2020 | | Saint Louis FC at Atlanta United 2 | Ppd. |
| June 6, 2020 | | Charlotte Independence at Saint Louis FC | Ppd. |
| June 13, 2020 | | Louisville City FC at Saint Louis FC | Ppd. |
| June 20, 2020 | | Saint Louis FC at North Carolina FC | Ppd. |
| June 25, 2020 | | Saint Louis FC at Hartford Athletic | Ppd. |
| June 28, 2020 | | Memphis 901 FC at Saint Louis FC | Ppd. |
| July 4, 2020 | | Saint Louis FC at Tampa Bay Rowdies | Ppd. |
| July 11, 2020 | L | Saint Louis FC 0 at Indy Eleven 2 | Final |
| July 18, 2020 | W | Saint Louis FC 1 at Louisville City FC 0 | Final |
| July 26, 2020 | W | Indy Eleven 0 at Saint Louis FC 1 | Final |
| August 1, 2020 | L | Saint Louis FC 0 at Memphis 901 FC 1 | Final |
| August 5, 2020 | T | Sporting Kansas City II 1 at Saint Louis FC 1 | Final |
| August 8, 2020 | L | FC Tulsa 2 at Saint Louis FC 0 | Final |
| August 15, 2020 | W | Sporting Kansas City II 1 at Saint Louis FC 3 | Final (7) |
| August 22, 2020 | T | Saint Louis FC 2 at Pittsburgh Riverhounds SC 2 | Final |
| August 29, 2020 | T | Louisville City FC 1 at Saint Louis FC 1 | Final |
| September 4, 2020 | W | Sporting Kansas City II 2 at Saint Louis FC 3 | Final |
| September 12, 2020 | L | Saint Louis FC 0 at Louisville City FC 3 | Final |
| September 19, 2020 | W | Sporting Kansas City II 2 at Saint Louis FC 3 | Final |
| September 23, 2020 | T | Saint Louis FC 1 at Indy Eleven 1 | Final |
| September 26, 2020 | L | Louisville City FC 1 at Saint Louis FC 0 | Final |
| October 3, 2020 | W | Indy Eleven 1 at Saint Louis FC 2 | Final |
| October 10, 2020 | W | Saint Louis FC 1 at Hartford Athletic 0 (Playoff) | Final |
| October 17, 2020 | L | Saint Louis FC 0 at Louisville City FC 2 (Playoff) | Final |
Saint Louis FC Schedule
