| | | |
| February 22, 2025 | L | Chicago Fire FC 2 at Columbus Crew SC 4 | Final |
| March 1, 2025 | T | D.C. United 2 at Chicago Fire FC 2 | Final |
| March 8, 2025 | W | Chicago Fire FC 3 at FC Dallas 1 | Final |
| March 15, 2025 | W | Chicago Fire FC 2 at Toronto FC 1 | Final |
| March 22, 2025 | W | Chicago Fire FC 3 at Vancouver Whitecaps FC 1 | Final |
| March 29, 2025 | T | Club de Foot Montreal 1 at Chicago Fire FC 1 | Final |
| April 5, 2025 | L | Chicago Fire FC 1 at New York Red Bulls 2 | Final |
| April 13, 2025 | T | Inter Miami CF 0 at Chicago Fire FC 0 | Final |
| April 19, 2025 | L | FC Cincinnati 3 at Chicago Fire FC 2 | Final |
| April 26, 2025 | L | Chicago Fire FC 2 at Nashville SC 7 | Final |
| May 3, 2025 | T | Orlando City SC 0 at Chicago Fire FC 0 | Final |
| May 10, 2025 | W | Atlanta United FC 1 at Chicago Fire FC 2 | Final |
| May 17, 2025 | W | Chicago Fire FC 4 at Charlotte FC 1 | Final |
| May 25, 2025 | L | Chicago Fire FC 1 at New York City FC 3 | Final |
| May 31, 2025 | W | Chicago Fire FC 3 at Orlando City SC 1 | Final |
| June 7, 2025 | W | Chicago Fire FC 7 at D.C. United 1 | Final |
| June 14, 2025 | L | Nashville SC 2 at Chicago Fire FC 0 | Final |
| June 25, 2025 | L | Philadelphia Union 1 at Chicago Fire FC 0 | Final |
| June 28, 2025 | W | Charlotte FC 2 at Chicago Fire FC 3 | Final |
| July 5, 2025 | L | Chicago Fire FC 1 at FC Cincinnati 2 | Final |
| July 12, 2025 | L | San Diego FC 2 at Chicago Fire FC 1 | Final |
| July 16, 2025 | T | Chicago Fire FC 2 at Atlanta United FC 2 | Final |
| July 19, 2025 | W | Chicago Fire FC 2 at Club de Foot Montreal 0 | Final |
| July 26, 2025 | W | New York Red Bulls 0 at Chicago Fire FC 1 | Final |
| August 9, 2025 | T | Los Angeles FC 2 at Chicago Fire FC 2 | Final |
| August 16, 2025 | W | St. Louis City SC 2 at Chicago Fire FC 3 | Final |
| August 23, 2025 | L | Chicago Fire FC 0 at Philadelphia Union 4 | Final |
| September 6, 2025 | W | New England Revolution 2 at Chicago Fire FC 3 | Final |
| September 13, 2025 | L | New York City FC 3 at Chicago Fire FC 1 | Final |
| September 20, 2025 | W | Chicago Fire FC 3 at Minnesota United FC 0 | Final |
| September 27, 2025 | W | Columbus Crew SC 0 at Chicago Fire FC 2 | Final |
| September 30, 2025 | W | Chicago Fire FC 5 at Inter Miami CF 3 | Final |
| October 4, 2025 | T | Toronto FC 2 at Chicago Fire FC 2 | Final |
| October 18, 2025 | T | Chicago Fire FC 2 at New England Revolution 2 | Final |
| October 22, 2025 | | Orlando City SC at Chicago Fire FC (Playoff) | 8:30 p.m. |
Chicago Fire FC Schedule
