| | | |
| March 8, 2025 | W | Detroit City FC 2 at Miami FC 0 | Final |
| March 15, 2025 | W | Detroit City FC 2 at Colorado Springs Switchbacks FC 1 | Final |
| March 22, 2025 | T | Birmingham Legion FC 2 at Detroit City FC 2 | Final |
| March 29, 2025 | L | Detroit City FC 0 at Louisville City FC 2 | Final |
| April 5, 2025 | T | Monterey Bay FC 0 at Detroit City FC 0 | Final |
| April 12, 2025 | W | Detroit City FC 3 at Phoenix Rising FC 2 | Final |
| April 19, 2025 | W | Rhode Island FC 0 at Detroit City FC 2 | Final |
| April 26, 2025 | W | Pittsburgh Riverhounds SC 0 at Detroit City FC 1 (Jagermeister Cup) | Final |
| May 3, 2025 | T | Detroit City FC 2 at Indy Eleven 2 | Final |
| May 10, 2025 | T | Detroit City FC 0 at Hartford Athletic 0 | Final |
| May 18, 2025 | T | Detroit City FC 1 at Birmingham Legion FC 1 | Final |
| May 24, 2025 | L | Charleston Battery 3 at Detroit City FC 1 | Final |
| May 31, 2025 | L | Detroit City FC 2 at Portland Hearts of Pine 4 (Jagermeister Cup) | Final |
| June 7, 2025 | L | Detroit City FC 0 at Pittsburgh Riverhounds SC 2 | Final |
| June 14, 2025 | L | Detroit City FC 2 at Loudoun United FC 3 | Final |
| June 21, 2025 | W | Miami FC 0 at Detroit City FC 2 | Final |
| June 28, 2025 | T | Detroit City FC 2 at Hartford Athletic 2 | Final |
| July 5, 2025 | L | Detroit City FC 0 at Oakland Roots 2 | Final |
| July 12, 2025 | L | Hartford Athletic 2 at Detroit City FC 1 | Final |
| July 18, 2025 | T | Pittsburgh Riverhounds SC 0 at Detroit City FC 0 | Final |
| July 26, 2025 | W | Westchester SC 0 at Detroit City FC 3 (Jagermeister Cup) | Final |
| August 6, 2025 | L | Detroit City FC 0 at Rhode Island FC 1 | Final |
| August 9, 2025 | W | Indy Eleven 0 at Detroit City FC 1 | Final |
| August 15, 2025 | T | Detroit City FC 1 at North Carolina FC 1 | Final |
| August 23, 2025 | W | Tampa Bay Rowdies 0 at Detroit City FC 2 | Final |
| August 30, 2025 | T | San Antonio FC 1 at Detroit City FC 1 | Final |
| September 6, 2025 | W | Orange County SC 1 at Detroit City FC 4 | Final |
| September 10, 2025 | L | Detroit City FC 0 at New Mexico United 4 | Final |
| September 20, 2025 | | Lexington Sporting Club at Detroit City FC | 7:00 p.m. |
| September 27, 2025 | | Louisville City FC at Detroit City FC | 7:00 p.m. |
| October 4, 2025 | | North Carolina FC at Detroit City FC | 4:00 p.m. |
| October 11, 2025 | | Loudoun United FC at Detroit City FC | 4:00 p.m. |
| October 18, 2025 | | Detroit City FC at Charleston Battery | 7:30 p.m. |
| October 25, 2025 | | Detroit City FC at Tampa Bay Rowdies | 7:30 p.m. |
Detroit City FC Schedule
