| | | |
| February 22, 2025 | T | Charlotte FC 2 at Seattle Sounders FC 2 | Final |
| March 1, 2025 | W | Atlanta United FC 0 at Charlotte FC 2 | Final |
| March 9, 2025 | L | Charlotte FC 0 at Inter Miami CF 1 | Final |
| March 15, 2025 | W | FC Cincinnati 0 at Charlotte FC 2 | Final |
| March 22, 2025 | W | San Jose Earthquakes 1 at Charlotte FC 4 | Final |
| March 29, 2025 | L | Charlotte FC 0 at Colorado Rapids 2 | Final |
| April 5, 2025 | W | Nashville SC 1 at Charlotte FC 2 | Final |
| April 12, 2025 | W | Charlotte FC 1 at Club de Foot Montreal 0 | Final |
| April 19, 2025 | W | San Diego FC 0 at Charlotte FC 3 | Final |
| April 26, 2025 | L | New England Revolution 1 at Charlotte FC 0 | Final |
| May 3, 2025 | L | Charlotte FC 2 at Columbus Crew SC 4 | Final |
| May 10, 2025 | L | Charlotte FC 1 at Nashville SC 2 | Final |
| May 14, 2025 | L | Charlotte FC 1 at Orlando City SC 3 | Final |
| May 17, 2025 | L | Chicago Fire FC 4 at Charlotte FC 1 | Final |
| May 24, 2025 | W | Columbus Crew SC 2 at Charlotte FC 3 | Final |
| May 28, 2025 | L | Charlotte FC 2 at New York Red Bulls 4 | Final |
| May 31, 2025 | W | Charlotte FC 2 at Toronto FC 0 | Final |
| June 14, 2025 | L | Charlotte FC 1 at Philadelphia Union 2 | Final |
| June 25, 2025 | L | Charlotte FC 1 at Sporting Kansas City 2 | Final |
| June 28, 2025 | L | Charlotte FC 2 at Chicago Fire FC 3 | Final |
| July 5, 2025 | T | Orlando City SC 2 at Charlotte FC 2 | Final |
| July 12, 2025 | W | New York City FC 0 at Charlotte FC 2 | Final |
| July 16, 2025 | W | D.C. United 1 at Charlotte FC 2 | Final |
| July 19, 2025 | W | Charlotte FC 3 at Atlanta United FC 2 | Final |
| July 26, 2025 | W | Toronto FC 0 at Charlotte FC 2 | Final |
| August 10, 2025 | W | Charlotte FC 1 at FC Cincinnati 0 | Final |
| August 16, 2025 | W | Real Salt Lake 0 at Charlotte FC 1 | Final |
| August 24, 2025 | W | New York Red Bulls 0 at Charlotte FC 1 | Final |
| August 30, 2025 | W | Charlotte FC 2 at New England Revolution 1 | Final |
| September 13, 2025 | W | Inter Miami CF 0 at Charlotte FC 3 | Final |
| September 20, 2025 | L | Charlotte FC 0 at New York City FC 2 | Final |
| September 27, 2025 | L | Club de Foot Montreal 4 at Charlotte FC 1 | Final |
| October 4, 2025 | W | Charlotte FC 1 at D.C. United 0 | Final |
| October 18, 2025 | W | Philadelphia Union 0 at Charlotte FC 2 | Final |
| October 28, 2025 | L | New York City FC 1 at Charlotte FC 0 (Playoff Gm. 1) | Final |
| November 1, 2025 | T | Charlotte FC 0 at New York City FC 0 (Playoff Gm. 2) | Final (PK 7-6) |
| November 7, 2025 | L | New York City FC 3 at Charlotte FC 1 (Playoff Gm. 3) | Final |
Charlotte FC Schedule
