| | | |
| March 8, 2025 | L | Tampa Bay Rowdies 0 at Las Vegas Lights FC 1 | Final |
| March 15, 2025 | L | Tampa Bay Rowdies 0 at FC Tulsa 1 | Final |
| March 22, 2025 | W | Tampa Bay Rowdies 2 at Miami FC 1 | Final |
| April 5, 2025 | L | Tampa Bay Rowdies 1 at Charleston Battery 2 | Final |
| April 12, 2025 | L | Loudoun United FC 2 at Tampa Bay Rowdies 1 | Final |
| April 19, 2025 | L | Louisville City FC 2 at Tampa Bay Rowdies 1 | Final |
| April 26, 2025 | T | Miami FC 3 at Tampa Bay Rowdies 3 (Jagermeister Cup) | Final |
| May 3, 2025 | T | Birmingham Legion FC 2 at Tampa Bay Rowdies 2 | Final |
| May 10, 2025 | L | Charleston Battery 3 at Tampa Bay Rowdies 1 | Final |
| May 17, 2025 | L | Rhode Island FC 3 at Tampa Bay Rowdies 0 | Final |
| May 24, 2025 | T | Tampa Bay Rowdies 1 at Lexington Sporting Club 1 | Final |
| May 31, 2025 | W | South Georgia Tormenta FC 1 at Tampa Bay Rowdies 2 (Jagermeister Cup) | Final |
| June 7, 2025 | L | Tampa Bay Rowdies 1 at Louisville City FC 2 | Final |
| June 14, 2025 | W | Miami FC 1 at Tampa Bay Rowdies 2 | Final |
| June 21, 2025 | L | Tampa Bay Rowdies 1 at Pittsburgh Riverhounds SC 2 | Final |
| June 25, 2025 | W | Indy Eleven 1 at Tampa Bay Rowdies 3 | Final |
| July 4, 2025 | W | Tampa Bay Rowdies 2 at FC Naples 0 (Jagermeister Cup) | Final |
| July 12, 2025 | L | Tampa Bay Rowdies 0 at San Antonio FC 1 | Final |
| July 16, 2025 | W | Tampa Bay Rowdies 1 at Hartford Athletic 0 | Final |
| July 26, 2025 | L | Tampa Bay Rowdies 1 at Charleston Battery 2 (Jagermeister Cup) | Final |
| August 2, 2025 | W | Tampa Bay Rowdies 3 at Indy Eleven 1 | Final |
| August 9, 2025 | L | Tampa Bay Rowdies 1 at North Carolina FC 2 | Final |
| August 16, 2025 | T | Phoenix Rising FC 1 at Tampa Bay Rowdies 1 | Final |
| August 23, 2025 | L | Tampa Bay Rowdies 0 at Detroit City FC 2 | Final |
| August 30, 2025 | W | Pittsburgh Riverhounds SC 1 at Tampa Bay Rowdies 2 | Final |
| September 6, 2025 | L | Sacramento Republic FC 2 at Tampa Bay Rowdies 0 | Final |
| September 13, 2025 | T | Colorado Springs Switchbacks FC 3 at Tampa Bay Rowdies 3 | Final |
| September 17, 2025 | W | Tampa Bay Rowdies 4 at Birmingham Legion FC 1 | Final |
| September 20, 2025 | T | North Carolina FC 2 at Tampa Bay Rowdies 2 | Final |
| September 28, 2025 | T | Tampa Bay Rowdies 2 at Loudoun United FC 2 | Final |
| October 4, 2025 | W | Monterey Bay FC 0 at Tampa Bay Rowdies 4 | Final |
| October 11, 2025 | L | Tampa Bay Rowdies 0 at Rhode Island FC 5 | Final |
| October 18, 2025 | W | Hartford Athletic 2 at Tampa Bay Rowdies 3 | Final |
| October 25, 2025 | T | Detroit City FC 1 at Tampa Bay Rowdies 1 | Final |
Tampa Bay Rowdies Schedule
