| | | |
| February 22, 2025 | L | Chicago Fire FC 2 at Columbus Crew SC 4 | Final |
| March 1, 2025 | T | D.C. United 2 at Chicago Fire FC 2 | Final |
| March 8, 2025 | W | Chicago Fire FC 3 at FC Dallas 1 | Final |
| March 15, 2025 | W | Chicago Fire FC 2 at Toronto FC 1 | Final |
| March 22, 2025 | W | Chicago Fire FC 3 at Vancouver Whitecaps FC 1 | Final |
| March 29, 2025 | T | Club de Foot Montreal 1 at Chicago Fire FC 1 | Final |
| April 5, 2025 | L | Chicago Fire FC 1 at New York Red Bulls 2 | Final |
| April 13, 2025 | T | Inter Miami CF 0 at Chicago Fire FC 0 | Final |
| April 19, 2025 | L | FC Cincinnati 3 at Chicago Fire FC 2 | Final |
| April 26, 2025 | L | Chicago Fire FC 2 at Nashville SC 7 | Final |
| May 3, 2025 | | Orlando City SC at Chicago Fire FC | 8:30 p.m. |
| May 10, 2025 | | Atlanta United FC at Chicago Fire FC | 2:30 p.m. |
| May 17, 2025 | | Chicago Fire FC at Charlotte FC | 7:30 p.m. |
| May 25, 2025 | | Chicago Fire FC at New York City FC | 3:00 p.m. |
| May 31, 2025 | | Chicago Fire FC at Orlando City SC | 7:30 p.m. |
| June 7, 2025 | | Chicago Fire FC at D.C. United | 7:30 p.m. |
| June 14, 2025 | | Nashville SC at Chicago Fire FC | 8:30 p.m. |
| June 25, 2025 | | Philadelphia Union at Chicago Fire FC | 8:30 p.m. |
| June 28, 2025 | | Charlotte FC at Chicago Fire FC | 8:30 p.m. |
| July 5, 2025 | | Chicago Fire FC at FC Cincinnati | 7:30 p.m. |
| July 12, 2025 | | San Diego FC at Chicago Fire FC | 8:30 p.m. |
| July 16, 2025 | | Chicago Fire FC at Atlanta United FC | 7:30 p.m. |
| July 19, 2025 | | Chicago Fire FC at Club de Foot Montreal | 7:30 p.m. |
| July 26, 2025 | | New York Red Bulls at Chicago Fire FC | 8:30 p.m. |
| August 9, 2025 | | Los Angeles FC at Chicago Fire FC | 8:30 p.m. |
| August 16, 2025 | | St. Louis City SC at Chicago Fire FC | 8:30 p.m. |
| August 23, 2025 | | Chicago Fire FC at Philadelphia Union | 7:30 p.m. |
| August 30, 2025 | | Chicago Fire FC at Inter Miami CF | 7:30 p.m. |
| September 6, 2025 | | New England Revolution at Chicago Fire FC | 8:30 p.m. |
| September 13, 2025 | | New York City FC at Chicago Fire FC | 8:30 p.m. |
| September 20, 2025 | | Chicago Fire FC at Minnesota United FC | 8:30 p.m. |
| September 27, 2025 | | Columbus Crew SC at Chicago Fire FC | 8:30 p.m. |
| October 4, 2025 | | Toronto FC at Chicago Fire FC | 8:30 p.m. |
| October 18, 2025 | | Chicago Fire FC at New England Revolution | 6:00 p.m. |
Chicago Fire FC Schedule
