| | | |
| March 7, 2025 | Colorado Rapids 2 2 at Austin FC II 4 | Final |
| March 7, 2025 | New York City FC II 2 at Crown Legacy FC 2 | Final (PK 5-4) |
| March 7, 2025 | Chattanooga FC 2 at Inter Miami CF II 1 | Final |
| March 7, 2025 | New York Red Bulls II 1 at Atlanta United 2 0 | Final |
| March 7, 2025 | Tacoma Defiance 2 at Ventura County FC 3 | Final |
| March 8, 2025 | Sporting Kansas City II 1 at Houston Dynamo 2 3 | Final |
| March 9, 2025 | Philadelphia Union II 0 at New England Revolution II 0 | Final (PK 3-4) |
| March 9, 2025 | Huntsville City Football Club 4 at Chicago Fire FC II 1 | Final |
| March 9, 2025 | Toronto FC II 1 at FC Cincinnati 2 0 | Final |
| March 9, 2025 | Columbus Crew 2 1 at Orlando City B 2 | Final |
| March 9, 2025 | St. Louis City SC 2 3 at North Texas SC 0 | Final |
| March 9, 2025 | Vancouver Whitecaps FC 2 3 at LAFC2 3 | Final (PK 4-2) |
| March 13, 2025 | FC Cincinnati 2 0 at New England Revolution II 2 | Final |
| March 13, 2025 | Carolina Core FC 1 at Inter Miami CF II 1 | Final (PK 4-2) |
| March 13, 2025 | Toronto FC II 0 at Chicago Fire FC II 1 | Final |
| March 14, 2025 | Minnesota United FC 2 1 at Austin FC II 0 | Final |
| March 14, 2025 | St. Louis City SC 2 1 at Sporting Kansas City II 0 | Final |
| March 14, 2025 | Columbus Crew 2 0 at New York City FC II 2 | Final |
| March 14, 2025 | Vancouver Whitecaps FC 2 2 at North Texas SC 3 | Final |
| March 14, 2025 | Ventura County FC 1 at LAFC2 3 | Final |
| March 14, 2025 | The Town FC 1 at Tacoma Defiance 3 | Final |
| March 15, 2025 | Huntsville City Football Club 0 at Chattanooga FC 1 | Final |
| March 15, 2025 | Real Monarchs 3 at Portland Timbers 2 2 | Final |
| March 16, 2025 | Atlanta United 2 2 at Orlando City B 2 | Final (PK 1-4) |
| March 17, 2025 | New York Red Bulls II 1 at Philadelphia Union II 1 | Final (PK 1-3) |
| March 23, 2025 | Sporting Kansas City II 0 at St. Louis City SC 2 2 | Final |
| March 23, 2025 | Columbus Crew 2 2 at Inter Miami CF II 4 | Final |
| March 23, 2025 | North Texas SC 1 at Houston Dynamo 2 5 | Final |
| March 23, 2025 | Colorado Rapids 2 2 at Real Monarchs 2 | Final (PK 6-7) |
| March 24, 2025 | Carolina Core FC 1 at New York City FC II 2 | Final |
| March 26, 2025 | Orlando City B 1 at Crown Legacy FC 0 | Final |
| March 26, 2025 | Houston Dynamo 2 0 at Ventura County FC 1 | Final |
| March 26, 2025 | The Town FC 1 at Portland Timbers 2 1 | Final (PK 3-4) |
| March 28, 2025 | Toronto FC II 0 at New England Revolution II 4 | Final |
| March 28, 2025 | Philadelphia Union II 2 at Huntsville City Football Club 2 | Final (PK 3-4) |
| March 28, 2025 | Sporting Kansas City II 3 at North Texas SC 3 | Final (PK 5-4) |
| March 29, 2025 | Portland Timbers 2 1 at Vancouver Whitecaps FC 2 1 | Final (PK 4-5) |
| March 30, 2025 | Chicago Fire FC II 2 at FC Cincinnati 2 1 | Final |
| March 30, 2025 | New York City FC II 2 at New York Red Bulls II 3 | Final |
| March 30, 2025 | Austin FC II 0 at St. Louis City SC 2 0 | Final (PK 4-5) |
| March 30, 2025 | Chattanooga FC 2 at Atlanta United 2 2 | Final (PK 4-3) |
| March 30, 2025 | Minnesota United FC 2 3 at Houston Dynamo 2 1 | Final |
| April 5, 2025 | North Texas SC 2 at Portland Timbers 2 1 | Final |
| April 5, 2025 | New York City FC II 0 at Carolina Core FC 5 | Final |
| April 5, 2025 | Orlando City B 0 at Chattanooga FC 2 | Final |
| April 6, 2025 | Inter Miami CF II 1 at Atlanta United 2 1 | Susp. |
| April 6, 2025 | Chicago Fire FC II 2 at New York Red Bulls II 3 | Final |
| April 6, 2025 | Colorado Rapids 2 1 at St. Louis City SC 2 0 | Final |
| April 6, 2025 | Ventura County FC 2 at Real Monarchs 1 | Final |
| April 6, 2025 | Crown Legacy FC 1 at Columbus Crew 2 1 | Final (PK 4-3) |
| April 6, 2025 | LAFC2 0 at Houston Dynamo 2 1 | Final |
| April 6, 2025 | Vancouver Whitecaps FC 2 3 at Tacoma Defiance 5 | Final |
| April 7, 2025 | Inter Miami CF II 2 at Atlanta United 2 3 | Final |
| April 9, 2025 | New England Revolution II 0 at Orlando City B 1 | Final |
| April 9, 2025 | Minnesota United FC 2 4 at Sporting Kansas City II 2 | Final |
| April 9, 2025 | FC Cincinnati 2 2 at New York City FC II 2 | Final (PK 5-3) |
| April 10, 2025 | Toronto FC II 1 at Inter Miami CF II 0 | Final |
| April 10, 2025 | Atlanta United 2 1 at Philadelphia Union II 5 | Final |
| April 10, 2025 | The Town FC 1 at Real Monarchs 2 | Final |
| April 10, 2025 | Tacoma Defiance 2 at LAFC2 3 | Final |
| April 11, 2025 | North Texas SC 1 at Austin FC II 1 | Final (PK 4-2) |
| April 13, 2025 | Chattanooga FC 4 at Carolina Core FC 1 | Final |
| April 13, 2025 | Colorado Rapids 2 2 at Portland Timbers 2 1 | Final |
| April 13, 2025 | Huntsville City Football Club 1 at Crown Legacy FC 1 | Final (PK 4-3) |
| April 13, 2025 | St. Louis City SC 2 1 at Ventura County FC 3 | Final |
| April 15, 2025 | Orlando City B 2 at Inter Miami CF II 3 | Final |
| April 18, 2025 | Carolina Core FC 1 at Toronto FC II 1 | Final (PK 4-1) |
| April 19, 2025 | Crown Legacy FC 1 at Chattanooga FC 2 | Final |
| April 19, 2025 | Austin FC II 0 at Vancouver Whitecaps FC 2 4 | Final |
| April 20, 2025 | New York Red Bulls II 1 at FC Cincinnati 2 3 | Final |
| April 20, 2025 | Chicago Fire FC II 0 at Huntsville City Football Club 5 | Final |
| April 20, 2025 | Colorado Rapids 2 3 at North Texas SC 2 | Final |
| April 23, 2025 | LAFC2 1 at Real Monarchs 1 | Final (PK 7-6) |
| April 23, 2025 | Columbus Crew 2 2 at New England Revolution II 2 | Final (PK 1-4) |
| April 23, 2025 | Tacoma Defiance 4 at Sporting Kansas City II 1 | Final |
| April 23, 2025 | Carolina Core FC 2 at Atlanta United 2 2 | Final (PK 4-5) |
| April 23, 2025 | Philadelphia Union II 4 at New York City FC II 1 | Final |
| April 23, 2025 | The Town FC 0 at North Texas SC 2 | Final |
| April 23, 2025 | Houston Dynamo 2 3 at Vancouver Whitecaps FC 2 1 | Final |
| April 23, 2025 | Portland Timbers 2 3 at Ventura County FC 2 | Final |
| April 24, 2025 | Minnesota United FC 2 2 at Colorado Rapids 2 0 | Final |
| April 25, 2025 | Chicago Fire FC II 0 at Toronto FC II 2 | Final |
| April 26, 2025 | New York City FC II 1 at New England Revolution II 2 | Final |
| April 26, 2025 | Crown Legacy FC 2 at Carolina Core FC 1 | Final |
| April 26, 2025 | St. Louis City SC 2 1 at Tacoma Defiance 4 | Final |
| April 27, 2025 | Atlanta United 2 0 at FC Cincinnati 2 0 | Final (PK 3-5) |
| April 27, 2025 | Chattanooga FC 0 at Columbus Crew 2 3 | Final |
| April 27, 2025 | Austin FC II 1 at Houston Dynamo 2 2 | Final |
| April 27, 2025 | Inter Miami CF II 0 at Huntsville City Football Club 4 | Final |
| April 27, 2025 | New York Red Bulls II 4 at Orlando City B 2 | Final |
| April 27, 2025 | Sporting Kansas City II 0 at Colorado Rapids 2 3 | Final |
| April 27, 2025 | LAFC2 1 at The Town FC 5 | Final |
| April 27, 2025 | Ventura County FC 3 at Vancouver Whitecaps FC 2 2 | Final |
| May 2, 2025 | North Texas SC 1 at Minnesota United FC 2 0 | Final |
| May 2, 2025 | Orlando City B 1 at Chicago Fire FC II 5 | Final |
| May 2, 2025 | Tacoma Defiance 2 at Ventura County FC 1 | Final |
| May 2, 2025 | Sporting Kansas City II 0 at Austin FC II 0 | Final (PK 6-7) |
| May 3, 2025 | Real Monarchs 1 at Vancouver Whitecaps FC 2 1 | Final (PK 1-3) |
| May 3, 2025 | Philadelphia Union II 0 at Chattanooga FC 0 | Final (PK 9-8) |
| May 3, 2025 | Atlanta United 2 2 at Huntsville City Football Club 2 | Final (PK 5-4) |
| May 4, 2025 | New England Revolution II 3 at Toronto FC II 3 | Final (PK 9-8) |
| May 4, 2025 | Carolina Core FC 2 at New York Red Bulls II 2 | Final (PK 6-7) |
| May 4, 2025 | Houston Dynamo 2 1 at St. Louis City SC 2 3 | Final |
| May 4, 2025 | New York City FC II 2 at Inter Miami CF II 4 | Final |
| May 4, 2025 | Portland Timbers 2 0 at The Town FC 5 | Final |
| May 4, 2025 | Colorado Rapids 2 2 at LAFC2 3 | Final |
| May 9, 2025 | Vancouver Whitecaps FC 2 0 at Portland Timbers 2 1 | Final |
| May 9, 2025 | Inter Miami CF II 2 at Crown Legacy FC 4 | Final |
| May 9, 2025 | Colorado Rapids 2 0 at Houston Dynamo 2 0 | Final (PK 4-5) |
| May 9, 2025 | Chattanooga FC 2 at Toronto FC II 1 | Final |
| May 9, 2025 | New York Red Bulls II 2 at New York City FC II 5 | Final |
| May 9, 2025 | FC Cincinnati 2 1 at Chicago Fire FC II 2 | Final |
| May 9, 2025 | LAFC2 1 at Ventura County FC 2 | Final |
| May 10, 2025 | Huntsville City Football Club 0 at Carolina Core FC 0 | Final (PK 3-2) |
| May 11, 2025 | Columbus Crew 2 0 at Philadelphia Union II 3 | Final |
| May 11, 2025 | St. Louis City SC 2 0 at The Town FC 4 | Final |
| May 13, 2025 | Austin FC II 1 at Ventura County FC 0 | Final |
| May 14, 2025 | Tacoma Defiance 0 at Vancouver Whitecaps FC 2 5 | Final |
| May 14, 2025 | LAFC2 0 at Portland Timbers 2 3 | Final |
| May 15, 2025 | Toronto FC II 0 at New York City FC II 3 | Final |
| May 17, 2025 | Inter Miami CF II 0 at Orlando City B 3 | Final |
| May 17, 2025 | Chattanooga FC 2 at Huntsville City Football Club 1 | Final |
| May 18, 2025 | The Town FC 0 at Sporting Kansas City II 0 | Final (PK 5-4) |
| May 18, 2025 | FC Cincinnati 2 0 at Columbus Crew 2 1 | Final |
| May 18, 2025 | St. Louis City SC 2 3 at Minnesota United FC 2 2 | Final |
| May 18, 2025 | Real Monarchs 1 at Colorado Rapids 2 2 | Final |
| May 18, 2025 | New England Revolution II 1 at New York Red Bulls II 4 | Final |
| May 18, 2025 | Ventura County FC 2 at Vancouver Whitecaps FC 2 0 | Final |
| May 18, 2025 | Houston Dynamo 2 1 at North Texas SC 2 | Final |
| May 18, 2025 | Tacoma Defiance 1 at Portland Timbers 2 2 | Final |
| May 19, 2025 | Crown Legacy FC 2 at Atlanta United 2 2 | Final (PK 5-3) |
| May 23, 2025 | Chicago Fire FC II 2 at New York City FC II 2 | Final (PK 2-4) |
| May 23, 2025 | LAFC2 3 at Sporting Kansas City II 0 | Final |
| May 23, 2025 | Huntsville City Football Club 2 at Toronto FC II 1 | Final |
| May 23, 2025 | Portland Timbers 2 3 at St. Louis City SC 2 6 | Final |
| May 23, 2025 | Minnesota United FC 2 1 at Austin FC II 1 | Final (PK 2-4) |
| May 24, 2025 | New England Revolution II 0 at Chattanooga FC 1 | Final |
| May 25, 2025 | Inter Miami CF II 1 at Philadelphia Union II 0 | Final |
| May 25, 2025 | New York Red Bulls II 1 at Columbus Crew 2 0 | Final |
| May 25, 2025 | Orlando City B 0 at Atlanta United 2 3 | Final |
| May 25, 2025 | FC Cincinnati 2 0 at Crown Legacy FC 2 | Final |
| May 25, 2025 | The Town FC 3 at Colorado Rapids 2 0 | Final |
| May 25, 2025 | North Texas SC 3 at Tacoma Defiance 2 | Final |
| May 28, 2025 | Portland Timbers 2 1 at Real Monarchs 4 | Final |
| May 28, 2025 | Vancouver Whitecaps FC 2 3 at Minnesota United FC 2 2 | Final |
| May 30, 2025 | Atlanta United 2 0 at New York Red Bulls II 1 | Final |
| May 30, 2025 | Crown Legacy FC 0 at Toronto FC II 1 | Final |
| May 30, 2025 | Houston Dynamo 2 1 at Austin FC II 1 | Final (PK 4-3) |
| May 31, 2025 | New England Revolution II 1 at New York City FC II 0 | Final |
| May 31, 2025 | Minnesota United FC 2 1 at Tacoma Defiance 1 | Final (PK 3-4) |
| June 1, 2025 | Colorado Rapids 2 4 at Sporting Kansas City II 1 | Final |
| June 1, 2025 | Columbus Crew 2 0 at Chicago Fire FC II 5 | Final |
| June 1, 2025 | Carolina Core FC 2 at Inter Miami CF II 1 | Final |
| June 1, 2025 | Chattanooga FC 1 at Orlando City B 2 | Final |
| June 1, 2025 | The Town FC 2 at LAFC2 1 | Final |
| June 1, 2025 | Real Monarchs 3 at Ventura County FC 3 | Final (PK 3-4) |
| June 3, 2025 | Philadelphia Union II 4 at FC Cincinnati 2 1 | Final |
| June 4, 2025 | New York Red Bulls II 5 at Crown Legacy FC 2 | Final |
| June 4, 2025 | North Texas SC 0 at St. Louis City SC 2 2 | Final |
| June 6, 2025 | New York City FC II 3 at Atlanta United 2 2 | Final |
| June 6, 2025 | Orlando City B 3 at Huntsville City Football Club 2 | Final |
| June 7, 2025 | Inter Miami CF II 3 at Chattanooga FC 3 | Final (PK 4-5) |
| June 7, 2025 | Sporting Kansas City II 1 at Houston Dynamo 2 1 | Final (PK 4-5) |
| June 8, 2025 | Toronto FC II 1 at FC Cincinnati 2 1 | Final (PK 2-4) |
| June 8, 2025 | Austin FC II 0 at Colorado Rapids 2 1 | Final |
| June 8, 2025 | Vancouver Whitecaps FC 2 at The Town FC | Ppd. |
| June 9, 2025 | New England Revolution II 2 at Chicago Fire FC II 2 | Final (PK 5-4) |
| June 11, 2025 | Huntsville City Football Club 1 at Atlanta United 2 0 | Final |
| June 11, 2025 | Orlando City B 0 at Carolina Core FC 1 | Final |
| June 11, 2025 | Tacoma Defiance 1 at Real Monarchs 2 | Final |
| June 11, 2025 | Ventura County FC 3 at Portland Timbers 2 2 | Final |
| June 12, 2025 | New York City FC II 1 at Columbus Crew 2 1 | Final (PK 4-5) |
| June 12, 2025 | Inter Miami CF II 0 at Toronto FC II 2 | Final |
| June 12, 2025 | Houston Dynamo 2 1 at Minnesota United FC 2 4 | Final |
| June 12, 2025 | LAFC2 0 at Vancouver Whitecaps FC 2 2 | Final |
| June 14, 2025 | Chicago Fire FC II 1 at Carolina Core FC 1 | Final (PK 5-4) |
| June 14, 2025 | Austin FC II 2 at North Texas SC 1 | Final |
| June 15, 2025 | FC Cincinnati 2 1 at New England Revolution II 0 | Final |
| June 15, 2025 | Chattanooga FC 2 at New York Red Bulls II 5 | Final |
| June 15, 2025 | Crown Legacy FC 0 at Philadelphia Union II 3 | Final |
| June 15, 2025 | St. Louis City SC 2 3 at Colorado Rapids 2 2 | Final |
| June 15, 2025 | Ventura County FC 2 at Minnesota United FC 2 0 | Final |
| June 15, 2025 | Houston Dynamo 2 1 at Real Monarchs 1 | Final (PK 4-5) |
| June 18, 2025 | North Texas SC 1 at Colorado Rapids 2 1 | Final (PK 4-2) |
| June 20, 2025 | Crown Legacy FC 3 at Huntsville City Football Club 3 | Final (PK 4-3) |
| June 20, 2025 | Austin FC II 1 at Portland Timbers 2 1 | Final (PK 5-4) |
| June 21, 2025 | Philadelphia Union II 3 at Columbus Crew 2 1 | Final |
| June 21, 2025 | FC Cincinnati 2 0 at Chattanooga FC 0 | Final (PK 8-7) |
| June 21, 2025 | North Texas SC 3 at Vancouver Whitecaps FC 2 4 | Final |
| June 21, 2025 | New York Red Bulls II 2 at Carolina Core FC 1 | Final |
| June 22, 2025 | The Town FC 1 at St. Louis City SC 2 3 | Final |
| June 25, 2025 | Chicago Fire FC II 3 at Inter Miami CF II 1 | Final |
| June 25, 2025 | Toronto FC II 2 at Orlando City B 1 | Final |
| June 25, 2025 | Minnesota United FC 2 0 at LAFC2 1 | Final |
| June 26, 2025 | New England Revolution II 2 at Philadelphia Union II 2 | Final (PK 6-7) |
| June 27, 2025 | Sporting Kansas City II 0 at Austin FC II 1 | Final |
| June 28, 2025 | Carolina Core FC 0 at Columbus Crew 2 1 | Final |
| June 28, 2025 | Houston Dynamo 2 0 at St. Louis City SC 2 1 | Final |
| June 28, 2025 | Colorado Rapids 2 1 at Minnesota United FC 2 2 | Final |
| June 29, 2025 | Huntsville City Football Club at New England Revolution II | Ppd. |
| June 29, 2025 | FC Cincinnati 2 3 at New York Red Bulls II 3 | Final (PK 5-3) |
| June 29, 2025 | Real Monarchs 3 at Vancouver Whitecaps FC 2 2 | Final |
| June 29, 2025 | Atlanta United 2 at Inter Miami CF II | Ppd. |
| June 29, 2025 | Ventura County FC 1 at The Town FC 1 | Final (PK 3-4) |
| June 29, 2025 | Tacoma Defiance 3 at LAFC2 2 | Final |
| June 30, 2025 | Orlando City B 1 at Philadelphia Union II 1 | Final (PK 2-4) |
| June 30, 2025 | Crown Legacy FC 1 at Chicago Fire FC II 1 | Final (PK 3-5) |
| July 3, 2025 | Minnesota United FC 2 1 at North Texas SC 3 | Final |
| July 3, 2025 | The Town FC 4 at Tacoma Defiance 4 | Final (PK 3-5) |
| July 3, 2025 | Vancouver Whitecaps FC 2 2 at Ventura County FC 1 | Final |
| July 4, 2025 | Inter Miami CF II 2 at Carolina Core FC 1 | Final |
| July 4, 2025 | LAFC2 1 at Austin FC II 4 | Final |
| July 5, 2025 | Portland Timbers 2 4 at Sporting Kansas City II 0 | Final |
| July 5, 2025 | New England Revolution II 2 at Columbus Crew 2 1 | Final |
| July 5, 2025 | Chicago Fire FC II 1 at Chattanooga FC 0 | Final |
| July 6, 2025 | Toronto FC II 0 at Philadelphia Union II 5 | Final |
| July 6, 2025 | Orlando City B 2 at Crown Legacy FC 2 | Final (PK 3-1) |
| July 6, 2025 | New York City FC II 2 at FC Cincinnati 2 2 | Final (PK 4-5) |
| July 6, 2025 | Huntsville City Football Club 2 at New York Red Bulls II 1 | Final |
| July 6, 2025 | Houston Dynamo 2 1 at Colorado Rapids 2 2 | Final |
| July 6, 2025 | St. Louis City SC 2 3 at Real Monarchs 1 | Final |
| July 9, 2025 | Carolina Core FC 0 at Crown Legacy FC 0 | Final (PK 4-2) |
| July 9, 2025 | FC Cincinnati 2 0 at Atlanta United 2 0 | Final (PK 4-3) |
| July 11, 2025 | New York Red Bulls II 2 at Toronto FC II 2 | Final (PK 4-3) |
| July 11, 2025 | St. Louis City SC 2 4 at Austin FC II 2 | Final |
| July 13, 2025 | New England Revolution II 4 at Chicago Fire FC II 3 | Final |
| July 13, 2025 | Chattanooga FC 3 at Philadelphia Union II 3 | Final (PK 5-6) |
| July 13, 2025 | Real Monarchs 1 at Minnesota United FC 2 2 | Final |
| July 13, 2025 | Huntsville City Football Club 0 at FC Cincinnati 2 1 | Final |
| July 13, 2025 | Crown Legacy FC 4 at Inter Miami CF II 3 | Final |
| July 13, 2025 | Carolina Core FC 1 at Orlando City B 3 | Final |
| July 13, 2025 | Columbus Crew 2 2 at Atlanta United 2 3 | Final |
| July 13, 2025 | Vancouver Whitecaps FC 2 0 at Colorado Rapids 2 0 | Final (PK 6-7) |
| July 13, 2025 | North Texas SC 0 at Houston Dynamo 2 3 | Final |
| July 13, 2025 | Sporting Kansas City II 1 at The Town FC 3 | Final |
| July 13, 2025 | Portland Timbers 2 0 at LAFC2 1 | Final |
| July 16, 2025 | Toronto FC II 0 at New York City FC II 1 | Final |
| July 16, 2025 | Ventura County FC 1 at St. Louis City SC 2 1 | Final (PK 2-4) |
| July 17, 2025 | Vancouver Whitecaps FC 2 4 at Houston Dynamo 2 2 | Final |
| July 17, 2025 | Real Monarchs 2 at Tacoma Defiance 2 | Final (PK 5-4) |
| July 18, 2025 | Columbus Crew 2 1 at Huntsville City Football Club 2 | Final |
| July 18, 2025 | North Texas SC 4 at Sporting Kansas City II 1 | Final |
| July 18, 2025 | Minnesota United FC 2 1 at Portland Timbers 2 1 | Final (PK 3-5) |
| July 19, 2025 | Inter Miami CF II 2 at Chattanooga FC 2 | Final (PK 4-5) |
| July 19, 2025 | Atlanta United 2 1 at Carolina Core FC 1 | Final (PK 4-5) |
| July 19, 2025 | Philadelphia Union II 4 at Crown Legacy FC 0 | Final |
| July 20, 2025 | Orlando City B 0 at New England Revolution II 3 | Final |
| July 20, 2025 | Chicago Fire FC II 3 at Toronto FC II 2 | Final |
| July 20, 2025 | New York City FC II 2 at New York Red Bulls II 4 | Final |
| July 20, 2025 | Tacoma Defiance 4 at Colorado Rapids 2 6 | Final |
| July 20, 2025 | Austin FC II 1 at Real Monarchs 1 | Final (PK 4-5) |
| July 20, 2025 | LAFC2 3 at Ventura County FC 3 | Final (PK 3-1) |
| July 22, 2025 | Minnesota United FC 2 2 at The Town FC 3 | Final |
| July 25, 2025 | Orlando City B 0 at Huntsville City Football Club 4 | Final |
| July 25, 2025 | Houston Dynamo 2 0 at Austin FC II 2 | Final |
| July 26, 2025 | Philadelphia Union II 2 at New York City FC II 0 | Final |
| July 26, 2025 | Crown Legacy FC 3 at Chattanooga FC 1 | Final |
| July 26, 2025 | The Town FC 4 at Real Monarchs 0 | Final |
| July 27, 2025 | New York Red Bulls II 2 at Chicago Fire FC II 9 | Final |
| July 27, 2025 | Toronto FC II 2 at Columbus Crew 2 1 | Final |
| July 27, 2025 | FC Cincinnati 2 3 at Inter Miami CF II 0 | Final |
| July 27, 2025 | Minnesota United FC 2 0 at St. Louis City SC 2 4 | Final |
| July 27, 2025 | Sporting Kansas City II 3 at LAFC2 1 | Final |
| July 27, 2025 | Portland Timbers 2 2 at Tacoma Defiance 1 | Final |
| July 30, 2025 | Atlanta United 2 0 at New England Revolution II 3 | Final |
| July 30, 2025 | Philadelphia Union II 2 at Carolina Core FC 3 | Final |
| July 31, 2025 | New York Red Bulls II 2 at Columbus Crew 2 1 | Final |
| July 31, 2025 | Sporting Kansas City II 3 at Vancouver Whitecaps FC 2 2 | Final |
| August 1, 2025 | Ventura County FC 6 at Tacoma Defiance 4 | Final |
| August 2, 2025 | Huntsville City Football Club 4 at Crown Legacy FC 0 | Final |
| August 2, 2025 | Toronto FC II 2 at Chattanooga FC 1 | Final |
| August 2, 2025 | New England Revolution II 0 at Carolina Core FC 1 | Final |
| August 2, 2025 | Houston Dynamo 2 2 at Minnesota United FC 2 2 | Final (PK 3-4) |
| August 2, 2025 | Colorado Rapids 2 4 at North Texas SC 1 | Final |
| August 3, 2025 | Austin FC II 3 at Sporting Kansas City II 1 | Final |
| August 3, 2025 | Columbus Crew 2 1 at Chicago Fire FC II 6 | Final |
| August 3, 2025 | FC Cincinnati 2 0 at Philadelphia Union II 1 | Final |
| August 3, 2025 | Atlanta United 2 1 at Inter Miami CF II 2 | Final |
| August 3, 2025 | New York City FC II 4 at Orlando City B 1 | Final |
| August 3, 2025 | The Town FC 4 at LAFC2 3 | Final |
| August 3, 2025 | Real Monarchs 2 at Portland Timbers 2 1 | Final |
| August 6, 2025 | St. Louis City SC 2 4 at Vancouver Whitecaps FC 2 2 | Final |
| August 8, 2025 | Atlanta United 2 3 at Toronto FC II 1 | Final |
| August 8, 2025 | Chicago Fire FC II 3 at New York City FC II 3 | Final (PK 4-1) |
| August 8, 2025 | Sporting Kansas City II 2 at Minnesota United FC 2 3 | Final |
| August 8, 2025 | North Texas SC 1 at Austin FC II 1 | Final (PK 6-5) |
| August 9, 2025 | Chattanooga FC 2 at Orlando City B 2 | Final (PK 4-2) |
| August 9, 2025 | Real Monarchs 4 at Houston Dynamo 2 0 | Final |
| August 9, 2025 | Vancouver Whitecaps FC 2 1 at The Town FC 2 | Final |
| August 9, 2025 | Portland Timbers 2 2 at Ventura County FC 2 | Final (PK 5-6) |
| August 10, 2025 | Carolina Core FC 2 at Huntsville City Football Club 3 | Final |
| August 10, 2025 | Colorado Rapids 2 2 at St. Louis City SC 2 2 | Final (PK 4-5) |
| August 10, 2025 | Columbus Crew 2 2 at New England Revolution II 2 | Final (PK 4-5) |
| August 10, 2025 | LAFC2 2 at Tacoma Defiance 4 | Final |
| August 11, 2025 | Crown Legacy FC 3 at FC Cincinnati 2 4 | Final |
| August 11, 2025 | Philadelphia Union II 0 at New York Red Bulls II 2 | Final |
| August 14, 2025 | Huntsville City Football Club 2 at Inter Miami CF II 2 | Final (PK 3-1) |
| August 15, 2025 | New York Red Bulls II 2 at New England Revolution II 4 | Final |
| August 15, 2025 | FC Cincinnati 2 0 at Toronto FC II 1 | Final |
| August 15, 2025 | Philadelphia Union II 0 at Chicago Fire FC II 2 | Final |
| August 15, 2025 | Austin FC II 1 at Colorado Rapids 2 4 | Final |
| August 15, 2025 | Tacoma Defiance 1 at Minnesota United FC 2 1 | Final (PK 2-3) |
| August 16, 2025 | Chattanooga FC 1 at Carolina Core FC 0 | Final |
| August 16, 2025 | Ventura County FC 1 at The Town FC 4 | Final |
| August 16, 2025 | St. Louis City SC 2 3 at North Texas SC 1 | Final |
| August 17, 2025 | Houston Dynamo 2 3 at Sporting Kansas City II 1 | Final |
| August 17, 2025 | Orlando City B 1 at Columbus Crew 2 2 | Final |
| August 17, 2025 | New York City FC II 1 at Huntsville City Football Club 2 | Final |
| August 17, 2025 | Crown Legacy FC 0 at Atlanta United 2 1 | Final |
| August 17, 2025 | LAFC2 1 at Real Monarchs 0 | Final |
| August 20, 2025 | Toronto FC II 2 at Philadelphia Union II 2 | Final (PK 8-7) |
| August 20, 2025 | Portland Timbers 2 0 at Colorado Rapids 2 1 | Final |
| August 21, 2025 | Vancouver Whitecaps FC 2 3 at LAFC2 1 | Final |
| August 22, 2025 | Inter Miami CF II 0 at Crown Legacy FC 6 | Final |
| August 22, 2025 | Columbus Crew 2 1 at New York City FC II 3 | Final |
| August 22, 2025 | The Town FC 1 at Houston Dynamo 2 0 | Final |
| August 22, 2025 | Ventura County FC 1 at Austin FC II 2 | Final |
| August 23, 2025 | Huntsville City Football Club 0 at Chattanooga FC 1 | Final |
| August 23, 2025 | Atlanta United 2 1 at Orlando City B 2 | Final |
| August 24, 2025 | New England Revolution II 3 at Philadelphia Union II 1 | Final |
| August 24, 2025 | Carolina Core FC 3 at FC Cincinnati 2 2 | Final |
| August 24, 2025 | Chicago Fire FC II 0 at New York Red Bulls II 1 | Final |
| August 24, 2025 | Sporting Kansas City II 0 at St. Louis City SC 2 3 | Final |
| August 24, 2025 | LAFC2 4 at North Texas SC 0 | Final |
| August 24, 2025 | Minnesota United FC 2 2 at Real Monarchs 3 | Final |
| August 24, 2025 | Tacoma Defiance 1 at Portland Timbers 2 2 | Final |
| August 27, 2025 | Inter Miami CF II 1 at Atlanta United 2 4 | Final |
| August 27, 2025 | Real Monarchs 0 at The Town FC 0 | Final (PK 2-3) |
| August 29, 2025 | Austin FC II 0 at LAFC2 1 | Final |
| August 30, 2025 | Huntsville City Football Club 0 at Atlanta United 2 2 | Final |
| August 31, 2025 | Colorado Rapids 2 3 at Sporting Kansas City II 2 | Final |
| August 31, 2025 | Chicago Fire FC II 2 at New England Revolution II 2 | Final (PK 3-1) |
| August 31, 2025 | New York City FC II 0 at Toronto FC II 3 | Final |
| August 31, 2025 | Columbus Crew 2 4 at New York Red Bulls II 7 | Final |
| August 31, 2025 | Philadelphia Union II 2 at FC Cincinnati 2 1 | Final |
| August 31, 2025 | Crown Legacy FC 0 at Orlando City B 1 | Final |
| August 31, 2025 | Portland Timbers 2 1 at Vancouver Whitecaps FC 2 4 | Final |
| August 31, 2025 | St. Louis City SC 2 2 at Houston Dynamo 2 2 | Final (PK 3-4) |
| August 31, 2025 | The Town FC 0 at Minnesota United FC 2 1 | Final |
| August 31, 2025 | Tacoma Defiance 0 at Real Monarchs 3 | Final |
| August 31, 2025 | North Texas SC 1 at Ventura County FC 0 | Final |
| September 4, 2025 | Carolina Core FC 4 at Crown Legacy FC 3 | Final |
| September 4, 2025 | Vancouver Whitecaps FC 2 3 at Tacoma Defiance 1 | Final |
| September 5, 2025 | Austin FC II 0 at Minnesota United FC 2 1 | Final |
| September 6, 2025 | Portland Timbers 2 2 at The Town FC 2 | Final (PK 3-4) |
| September 6, 2025 | Ventura County FC 1 at North Texas SC 1 | Final (PK 4-5) |
| September 6, 2025 | Real Monarchs 1 at Sporting Kansas City II 2 | Final |
| September 7, 2025 | Inter Miami CF II 0 at Columbus Crew 2 4 | Final |
| September 8, 2025 | Huntsville City Football Club 0 at New England Revolution II 1 | Final |
| September 10, 2025 | FC Cincinnati 2 1 at Chicago Fire FC II 2 | Final |
| September 12, 2025 | Toronto FC II 1 at New England Revolution II 2 | Final |
| September 12, 2025 | Chattanooga FC 4 at Crown Legacy FC 2 | Final |
| September 12, 2025 | Austin FC II 2 at Tacoma Defiance 1 | Final |
| September 13, 2025 | The Town FC 1 at Vancouver Whitecaps FC 2 3 | Final |
| September 13, 2025 | Minnesota United FC 2 1 at St. Louis City SC 2 1 | Final (PK 2-3) |
| September 13, 2025 | Atlanta United 2 2 at Carolina Core FC 4 | Final |
| September 13, 2025 | New York Red Bulls II 3 at Huntsville City Football Club 2 | Final |
| September 14, 2025 | Philadelphia Union II 3 at Columbus Crew 2 1 | Final |
| September 14, 2025 | LAFC2 3 at Portland Timbers 2 5 | Final |
| September 14, 2025 | Orlando City B 1 at Inter Miami CF II 1 | Final (PK 5-3) |
| September 14, 2025 | Houston Dynamo 2 1 at Colorado Rapids 2 0 | Final |
| September 14, 2025 | Sporting Kansas City II 0 at North Texas SC 1 | Final (PK 4-3) |
| September 14, 2025 | New York City FC II 5 at Chicago Fire FC II 4 | Final |
| September 14, 2025 | Ventura County FC 1 at Real Monarchs 2 | Final |
| September 17, 2025 | Tacoma Defiance 2 at Houston Dynamo 2 1 | Final |
| September 18, 2025 | New England Revolution II 3 at New York City FC II 1 | Final |
| September 19, 2025 | Vancouver Whitecaps FC 2 2 at Sporting Kansas City II 2 | Final (PK 4-2) |
| September 19, 2025 | Columbus Crew 2 0 at Toronto FC II 0 | Final (PK 3-4) |
| September 19, 2025 | Real Monarchs 3 at LAFC2 1 | Final |
| September 20, 2025 | Atlanta United 2 1 at Crown Legacy FC 0 | Final |
| September 21, 2025 | Chicago Fire FC II 2 at Philadelphia Union II 5 | Final |
| September 21, 2025 | North Texas SC 2 at Minnesota United FC 2 1 | Final |
| September 21, 2025 | Inter Miami CF II 1 at New York Red Bulls II 1 | Final (PK 4-2) |
| September 21, 2025 | New England Revolution II 1 at FC Cincinnati 2 3 | Final |
| September 21, 2025 | Carolina Core FC 0 at Chattanooga FC 0 | Final (PK 6-5) |
| September 21, 2025 | Huntsville City Football Club 1 at Orlando City B 0 | Final |
| September 21, 2025 | Portland Timbers 2 1 at Houston Dynamo 2 3 | Final |
| September 21, 2025 | St. Louis City SC 2 2 at Austin FC II 1 | Final |
| September 21, 2025 | Colorado Rapids 2 4 at Tacoma Defiance 1 | Final |
| September 21, 2025 | The Town FC 4 at Ventura County FC 0 | Final |
| September 24, 2025 | Chattanooga FC 1 at Atlanta United 2 0 | 72 Min. |
| September 25, 2025 | Columbus Crew 2 at FC Cincinnati 2 | 7:30 p.m. |
| September 26, 2025 | Crown Legacy FC at New England Revolution II | 3:00 p.m. |
| September 26, 2025 | Philadelphia Union II at Toronto FC II | 7:00 p.m. |
| September 26, 2025 | Portland Timbers 2 at Austin FC II | 8:30 p.m. |
| September 27, 2025 | Minnesota United FC 2 at Colorado Rapids 2 | 4:30 p.m. |
| September 27, 2025 | Orlando City B at Carolina Core FC | 7:30 p.m. |
| September 27, 2025 | Real Monarchs at North Texas SC | 8:30 p.m. |
| September 28, 2025 | Ventura County FC at Sporting Kansas City II | 1:00 p.m. |
| September 28, 2025 | Atlanta United 2 at Chicago Fire FC II | 2:00 p.m. |
| September 28, 2025 | LAFC2 at St. Louis City SC 2 | 5:00 p.m. |
| September 28, 2025 | Chattanooga FC at New York City FC II | 7:30 p.m. |
| September 28, 2025 | Inter Miami CF II at Huntsville City Football Club | 8:00 p.m. |
| September 28, 2025 | Tacoma Defiance at The Town FC | 8:00 p.m. |
| October 1, 2025 | New York Red Bulls II at FC Cincinnati 2 | 7:30 p.m. |
| October 5, 2025 | Atlanta United 2 at Chattanooga FC | 1:00 p.m. |
| October 5, 2025 | Chicago Fire FC II at Columbus Crew 2 | 1:00 p.m. |
| October 5, 2025 | Carolina Core FC at Huntsville City Football Club | 1:00 p.m. |
| October 5, 2025 | New England Revolution II at Inter Miami CF II | 1:00 p.m. |
| October 5, 2025 | Toronto FC II at New York Red Bulls II | 1:00 p.m. |
| October 5, 2025 | FC Cincinnati 2 at Orlando City B | 1:00 p.m. |
| October 5, 2025 | New York City FC II at Philadelphia Union II | 1:00 p.m. |
| October 5, 2025 | North Texas SC at LAFC2 | 4:00 p.m. |
| October 5, 2025 | Sporting Kansas City II at Minnesota United FC 2 | 4:00 p.m. |
| October 5, 2025 | St. Louis City SC 2 at Portland Timbers 2 | 4:00 p.m. |
| October 5, 2025 | Vancouver Whitecaps FC 2 at Real Monarchs | 4:00 p.m. |
| October 5, 2025 | Houston Dynamo 2 at Tacoma Defiance | 4:00 p.m. |
| October 5, 2025 | Austin FC II at The Town FC | 4:00 p.m. |
| October 5, 2025 | Colorado Rapids 2 at Ventura County FC | 4:00 p.m. |
MLS NEXT Pro Schedule
