| | | |
| March 8, 2025 | L | Tampa Bay Rowdies 0 at Las Vegas Lights FC 1 | Final |
| March 15, 2025 | L | Tampa Bay Rowdies 0 at FC Tulsa 1 | Final |
| March 22, 2025 | W | Tampa Bay Rowdies 2 at Miami FC 1 | Final |
| April 5, 2025 | L | Tampa Bay Rowdies 1 at Charleston Battery 2 | Final |
| April 12, 2025 | L | Loudoun United FC 2 at Tampa Bay Rowdies 1 | Final |
| April 19, 2025 | L | Louisville City FC 2 at Tampa Bay Rowdies 1 | Final |
| April 26, 2025 | T | Miami FC 3 at Tampa Bay Rowdies 3 (Jagermeister Cup) | Final |
| May 3, 2025 | T | Birmingham Legion FC 2 at Tampa Bay Rowdies 2 | Final |
| May 10, 2025 | | Charleston Battery at Tampa Bay Rowdies | 7:30 p.m. |
| May 17, 2025 | | Rhode Island FC at Tampa Bay Rowdies | 7:30 p.m. |
| May 24, 2025 | | Tampa Bay Rowdies at Lexington Sporting Club | 7:00 p.m. |
| May 31, 2025 | | South Georgia Tormenta FC at Tampa Bay Rowdies | 7:30 p.m. |
| June 7, 2025 | | Tampa Bay Rowdies at Louisville City FC | 8:00 p.m. |
| June 14, 2025 | | Miami FC at Tampa Bay Rowdies | 7:30 p.m. |
| June 21, 2025 | | Tampa Bay Rowdies at Pittsburgh Riverhounds SC | 7:00 p.m. |
| June 25, 2025 | | Indy Eleven at Tampa Bay Rowdies | 7:30 p.m. |
| July 4, 2025 | | Tampa Bay Rowdies at FC Naples | 8:00 p.m. |
| July 12, 2025 | | Tampa Bay Rowdies at San Antonio FC | 9:00 p.m. |
| July 16, 2025 | | Tampa Bay Rowdies at Hartford Athletic | 7:30 p.m. |
| July 26, 2025 | | Tampa Bay Rowdies at Charleston Battery | 7:30 p.m. |
| August 2, 2025 | | Tampa Bay Rowdies at Indy Eleven | 7:00 p.m. |
| August 9, 2025 | | Tampa Bay Rowdies at North Carolina FC | 7:30 p.m. |
| August 16, 2025 | | Phoenix Rising FC at Tampa Bay Rowdies | 7:30 p.m. |
| August 23, 2025 | | Tampa Bay Rowdies at Detroit City FC | 7:00 p.m. |
| August 30, 2025 | | Pittsburgh Riverhounds SC at Tampa Bay Rowdies | 7:30 p.m. |
| September 6, 2025 | | Sacramento Republic FC at Tampa Bay Rowdies | 7:30 p.m. |
| September 13, 2025 | | Colorado Springs Switchbacks FC at Tampa Bay Rowdies | 7:30 p.m. |
| September 17, 2025 | | Tampa Bay Rowdies at Birmingham Legion FC | 8:00 p.m. |
| September 20, 2025 | | North Carolina FC at Tampa Bay Rowdies | 7:30 p.m. |
| September 28, 2025 | | Tampa Bay Rowdies at Loudoun United FC | 5:00 p.m. |
| October 4, 2025 | | Monterey Bay FC at Tampa Bay Rowdies | 7:30 p.m. |
| October 11, 2025 | | Tampa Bay Rowdies at Rhode Island FC | 4:00 p.m. |
| October 18, 2025 | | Hartford Athletic at Tampa Bay Rowdies | 7:30 p.m. |
| October 25, 2025 | | Detroit City FC at Tampa Bay Rowdies | 7:30 p.m. |
Tampa Bay Rowdies Schedule
