| | | |
| March 8, 2025 | L | Oakland Roots 2 at Orange County SC 4 | Final |
| March 15, 2025 | L | Oakland Roots 2 at Monterey Bay FC 3 | Final |
| March 22, 2025 | L | San Antonio FC 2 at Oakland Roots 1 | Final |
| March 29, 2025 | T | Las Vegas Lights FC 0 at Oakland Roots 0 | Final |
| April 5, 2025 | L | Rhode Island FC 3 at Oakland Roots 0 | Final |
| April 12, 2025 | W | Oakland Roots 2 at FC Tulsa 1 | Final |
| April 19, 2025 | W | Orange County SC 0 at Oakland Roots 2 | Final |
| April 27, 2025 | L | Oakland Roots 1 at Spokane Velocity FC 2 (Jagermeister Cup) | Final |
| May 4, 2025 | L | Sacramento Republic FC 1 at Oakland Roots 0 | Final |
| May 10, 2025 | | Oakland Roots at San Antonio FC | 8:30 p.m. |
| May 16, 2025 | | Oakland Roots at North Carolina FC | 7:00 p.m. |
| May 24, 2025 | | New Mexico United at Oakland Roots | 10:00 p.m. |
| May 31, 2025 | | Oakland Roots at AV Alta FC | 10:30 p.m. |
| June 7, 2025 | | El Paso Locomotive FC at Oakland Roots | 10:00 p.m. |
| June 14, 2025 | | Oakland Roots at Colorado Springs Switchbacks FC | 9:00 p.m. |
| June 21, 2025 | | Oakland Roots at Birmingham Legion FC | 8:00 p.m. |
| June 28, 2025 | | Monterey Bay FC at Oakland Roots | 10:00 p.m. |
| July 5, 2025 | | Detroit City FC at Oakland Roots | 10:00 p.m. |
| July 12, 2025 | | Phoenix Rising FC at Oakland Roots | 10:00 p.m. |
| July 19, 2025 | | Oakland Roots at Loudoun United FC | 7:00 p.m. |
| July 26, 2025 | | Orange County SC at Oakland Roots | 4:00 p.m. |
| August 9, 2025 | | Colorado Springs Switchbacks FC at Oakland Roots | 10:00 p.m. |
| August 16, 2025 | | Oakland Roots at Sacramento Republic FC | 11:00 p.m. |
| August 23, 2025 | | Oakland Roots at Lexington Sporting Club | 7:00 p.m. |
| August 30, 2025 | | Monterey Bay FC at Oakland Roots | 10:00 p.m. |
| September 6, 2025 | | Pittsburgh Riverhounds SC at Oakland Roots | 10:00 p.m. |
| September 13, 2025 | | Oakland Roots at Charleston Battery | 7:30 p.m. |
| September 20, 2025 | | FC Tulsa at Oakland Roots | 10:00 p.m. |
| September 27, 2025 | | Oakland Roots at Phoenix Rising FC | 10:00 p.m. |
| October 1, 2025 | | Oakland Roots at El Paso Locomotive FC | 2:00 p.m. |
| October 4, 2025 | | Hartford Athletic at Oakland Roots | 10:00 p.m. |
| October 11, 2025 | | Oakland Roots at Las Vegas Lights FC | 10:30 p.m. |
| October 19, 2025 | | Oakland Roots at New Mexico United | 5:00 p.m. |
| October 25, 2025 | | Lexington Sporting Club at Oakland Roots | 10:00 p.m. |
Oakland Roots Schedule
