| | | |
| March 6, 2026 | W | Louisville City FC 2 at Lexington SC 1 | Final |
| March 14, 2026 | W | Miami FC 1 at Louisville City FC 4 | Final |
| March 21, 2026 | W | Rhode Island FC 2 at Louisville City FC 4 | Final |
| March 28, 2026 | W | Louisville City FC 1 at Brooklyn FC 0 | Final |
| April 4, 2026 | L | Charleston Battery 2 at Louisville City FC 0 | Final |
| April 10, 2026 | T | Louisville City FC 3 at Loudoun United FC 3 | Final |
| April 18, 2026 | W | Sporting Club Jacksonville 0 at Louisville City FC 1 | Final |
| April 25, 2026 | W | Fort Wayne FC 1 at Louisville City FC 3 (USL Cup) | Final |
| May 2, 2026 | L | Louisville City FC 1 at Detroit City FC 2 | Final |
| May 9, 2026 | L | Pittsburgh Riverhounds SC 2 at Louisville City FC 0 | Final |
| May 17, 2026 | W | Louisville City FC 5 at Union Omaha 1 (USL Cup) | Final |
| May 23, 2026 | L | Louisville City FC 3 at Miami FC 4 | Final |
| May 30, 2026 | L | Tampa Bay Rowdies 2 at Louisville City FC 0 | Final |
| June 3, 2026 | T | Louisville City FC 1 at Birmingham Legion FC 1 | Final |
| June 10, 2026 | W | Louisville City FC 2 at Phoenix Rising FC 0 | Final |
| June 13, 2026 | T | Brooklyn FC 2 at Louisville City FC 2 | Final |
| June 20, 2026 | T | Louisville City FC 0 at Detroit City FC 0 (USL Cup) | Final (PK 4-3) |
| July 4, 2026 | | Hartford Athletic at Louisville City FC | 8:00 p.m. |
| July 11, 2026 | | Lexington SC at Louisville City FC | 8:00 p.m. |
| July 18, 2026 | | Louisville City FC at Pittsburgh Riverhounds SC | 12:00 p.m. |
| July 29, 2026 | | Birmingham Legion FC at Louisville City FC | 7:30 p.m. |
| August 1, 2026 | | Louisville City FC at Tampa Bay Rowdies | 7:30 p.m. |
| August 8, 2026 | | San Antonio FC at Louisville City FC | 8:00 p.m. |
| August 15, 2026 | | Louisville City FC at Orange County SC | 10:00 p.m. |
| August 22, 2026 | | Louisville City FC at Indy Eleven | 7:00 p.m. |
| August 29, 2026 | | Detroit City FC at Louisville City FC | 8:00 p.m. |
| September 5, 2026 | | Louisville City FC at Rhode Island FC | 7:30 p.m. |
| September 12, 2026 | | El Paso Locomotive FC at Louisville City FC | 7:00 p.m. |
| September 19, 2026 | | Loudoun United FC at Louisville City FC | 7:00 p.m. |
| September 25, 2026 | | Louisville City FC at Hartford Athletic | 7:00 p.m. |
| October 3, 2026 | | Louisville City FC at Sporting Club Jacksonville | 5:00 p.m. |
| October 10, 2026 | | Louisville City FC at Charleston Battery | 6:00 p.m. |
| October 17, 2026 | | Indy Eleven at Louisville City FC | 7:00 p.m. |
| October 24, 2026 | | Detroit City FC at Louisville City FC | 7:00 p.m. |
Louisville City FC Schedule
