| | | |
| March 8, 2025 | W | Louisville City FC 2 at Charleston Battery 1 | Final |
| March 16, 2025 | T | Louisville City FC 1 at Birmingham Legion FC 1 | Final |
| March 22, 2025 | W | Loudoun United FC 0 at Louisville City FC 2 | Final |
| March 29, 2025 | W | Detroit City FC 0 at Louisville City FC 2 | Final |
| April 5, 2025 | T | Louisville City FC 1 at Sacramento Republic FC 1 | Final |
| April 19, 2025 | W | Louisville City FC 2 at Tampa Bay Rowdies 1 | Final |
| April 26, 2025 | W | Richmond Kickers 1 at Louisville City FC 4 (Jagermeister Cup) | Final |
| May 1, 2025 | W | Lexington Sporting Club 0 at Louisville City FC 2 | Final |
| May 10, 2025 | T | Pittsburgh Riverhounds SC 0 at Louisville City FC 0 | Final |
| May 17, 2025 | T | Louisville City FC 1 at Hartford Athletic 1 | Final |
| May 24, 2025 | W | Louisville City FC 2 at Monterey Bay FC 0 | Final |
| May 31, 2025 | L | Louisville City FC 1 at Loudoun United FC 2 | Final |
| June 7, 2025 | W | Tampa Bay Rowdies 1 at Louisville City FC 2 | Final |
| June 11, 2025 | W | Rhode Island FC 1 at Louisville City FC 2 | Final |
| June 14, 2025 | W | Birmingham Legion FC 2 at Louisville City FC 4 | Final |
| June 20, 2025 | L | Louisville City FC 1 at North Carolina FC 2 | Final |
| June 25, 2025 | W | Louisville City FC 4 at Loudoun United FC 1 | Final |
| June 29, 2025 | W | North Carolina FC 0 at Louisville City FC 1 (Jagermeister Cup) | Final |
| July 4, 2025 | W | Louisville City FC 1 at Colorado Springs Switchbacks FC 0 | Final |
| July 19, 2025 | T | FC Tulsa 1 at Louisville City FC 1 | Final |
| July 26, 2025 | W | Louisville City FC 2 at Lexington Sporting Club 1 (Jagermeister Cup) | Final |
| August 2, 2025 | W | North Carolina FC 1 at Louisville City FC 4 | Final |
| August 9, 2025 | W | Charleston Battery 1 at Louisville City FC 4 | Final |
| August 16, 2025 | W | Louisville City FC 4 at Miami FC 2 | Final |
| August 23, 2025 | T | New Mexico United 0 at Louisville City FC 0 | Final |
| August 30, 2025 | W | Louisville City FC 3 at Indy Eleven 1 | Final |
| September 6, 2025 | W | Louisville City FC 1 at Rhode Island FC 0 | Final |
| September 13, 2025 | W | Louisville City FC 2 at Pittsburgh Riverhounds SC 0 | Final |
| September 20, 2025 | W | Las Vegas Lights FC 1 at Louisville City FC 3 | Final |
| September 27, 2025 | W | Louisville City FC 1 at Detroit City FC 0 | Final |
| October 4, 2025 | W | Indy Eleven 0 at Louisville City FC 2 | Final |
| October 11, 2025 | W | Miami FC 0 at Louisville City FC 1 | Final |
| October 18, 2025 | T | Louisville City FC 0 at Orange County SC 0 | Final |
| October 25, 2025 | W | Hartford Athletic 0 at Louisville City FC 1 | Final |
| November 1, 2025 | | Detroit City FC at Louisville City FC (Playoff) | 7:00 p.m. |
Louisville City FC Schedule
