| | | |
| March 6, 2026 | | Louisville City FC at Lexington SC | 7:30 p.m. |
| March 14, 2026 | | Miami FC at Louisville City FC | 3:00 p.m. |
| March 21, 2026 | | Rhode Island FC at Louisville City FC | 3:00 p.m. |
| March 28, 2026 | | Louisville City FC at Brooklyn FC | 6:00 p.m. |
| April 4, 2026 | | Charleston Battery at Louisville City FC | 3:00 p.m. |
| April 10, 2026 | | Louisville City FC at Loudoun United FC | 5:30 p.m. |
| April 18, 2026 | | Sporting Club Jacksonville at Louisville City FC | 4:00 p.m. |
| April 25, 2026 | | Fort Wayne FC at Louisville City FC (USL Cup) | 6:00 p.m. |
| May 2, 2026 | | Louisville City FC at Detroit City FC | 6:00 p.m. |
| May 9, 2026 | | Pittsburgh Riverhounds SC at Louisville City FC | 6:00 p.m. |
| May 17, 2026 | | Louisville City FC at Union Omaha (USL Cup) | 4:00 p.m. |
| May 23, 2026 | | Louisville City FC at Miami FC | 6:00 p.m. |
| May 30, 2026 | | Tampa Bay Rowdies at Louisville City FC | 6:00 p.m. |
| June 3, 2026 | | Louisville City FC at Birmingham Legion FC | 7:00 p.m. |
| June 10, 2026 | | Louisville City FC at Phoenix Rising FC | 9:30 p.m. |
| June 13, 2026 | | Brooklyn FC at Louisville City FC | 7:00 p.m. |
| June 20, 2026 | | Louisville City FC at Detroit City FC | 3:00 p.m. |
| July 4, 2026 | | Hartford Athletic at Louisville City FC | 7:00 p.m. |
| July 11, 2026 | | Lexington SC at Louisville City FC | 7:00 p.m. |
| July 18, 2026 | | Louisville City FC at Pittsburgh Riverhounds SC | 6:00 p.m. |
| July 29, 2026 | | Birmingham Legion FC at Louisville City FC | 6:30 p.m. |
| August 1, 2026 | | Louisville City FC at Tampa Bay Rowdies | 6:30 p.m. |
| August 8, 2026 | | San Antonio FC at Louisville City FC | 7:00 p.m. |
| August 15, 2026 | | Louisville City FC at Orange County SC | 9:00 p.m. |
| August 22, 2026 | | Louisville City FC at Indy Eleven | 6:00 p.m. |
| August 29, 2026 | | Detroit City FC at Louisville City FC | 7:00 p.m. |
| September 5, 2026 | | Louisville City FC at Rhode Island FC | 6:30 p.m. |
| September 12, 2026 | | El Paso Locomotive FC at Louisville City FC | 6:00 p.m. |
| September 19, 2026 | | Loudoun United FC at Louisville City FC | 6:00 p.m. |
| September 25, 2026 | | Louisville City FC at Hartford Athletic | 6:00 p.m. |
| October 3, 2026 | | Louisville City FC at Sporting Club Jacksonville | 4:00 p.m. |
| October 10, 2026 | | Louisville City FC at Charleston Battery | 3:00 p.m. |
| October 17, 2026 | | Indy Eleven at Louisville City FC | 6:00 p.m. |
| October 24, 2026 | | Detroit City FC at Louisville City FC | 6:00 p.m. |
Louisville City FC Schedule
