| | | |
| November 30, 2024 | W | Las Vegas Desert Dogs 12 at Rochester Knighthawks 14 | Final |
| December 7, 2024 | L | Buffalo Bandits 15 at Rochester Knighthawks 7 | Final |
| December 13, 2024 | L | Rochester Knighthawks 7 at Vancouver Warriors 10 | Final |
| December 21, 2024 | L | Saskatchewan Rush 14 at Rochester Knighthawks 12 | Final |
| December 28, 2024 | L | Rochester Knighthawks 6 at Buffalo Bandits 13 | Final |
| January 4, 2025 | W | Rochester Knighthawks 16 at Georgia Swarm 9 | Final |
| January 11, 2025 | L | Halifax Thunderbirds 19 at Rochester Knighthawks 18 | Final |
| January 18, 2025 | W | Rochester Knighthawks 10 at Halifax Thunderbirds 9 | Final |
| January 25, 2025 | L | Colorado Mammoth 11 at Rochester Knighthawks 10 | Final |
| February 1, 2025 | W | Rochester Knighthawks 15 at Philadelphia Wings 12 | Final |
| February 15, 2025 | L | Rochester Knighthawks 9 at Saskatchewan Rush 17 | Final |
| February 22, 2025 | W | Rochester Knighthawks 15 at Calgary Roughnecks 10 | Final |
| February 28, 2025 | W | Albany FireWolves 7 at Rochester Knighthawks 14 | Final |
| March 7, 2025 | W | Rochester Knighthawks 21 at Las Vegas Desert Dogs 13 | Final |
| March 15, 2025 | W | Rochester Knighthawks 18 at Ottawa Black Bears 8 | Final |
| March 29, 2025 | W | San Diego Seals 13 at Rochester Knighthawks 14 | Final |
| April 5, 2025 | W | Ottawa Black Bears 6 at Rochester Knighthawks 12 | Final |
| April 19, 2025 | L | Toronto Rock 11 at Rochester Knighthawks 10 | Final |
| April 26, 2025 | L | Rochester Knighthawks 10 at Vancouver Warriors 15 (Playoff) | Final |
Rochester Knighthawks Schedule
