| | | |
| November 30, 2024 | W | Las Vegas Desert Dogs 12 at Rochester Knighthawks 14 | Final |
| December 7, 2024 | L | Buffalo Bandits 15 at Rochester Knighthawks 7 | Final |
| December 13, 2024 | L | Rochester Knighthawks 7 at Vancouver Warriors 10 | Final |
| December 21, 2024 | L | Saskatchewan Rush 14 at Rochester Knighthawks 12 | Final |
| December 28, 2024 | L | Rochester Knighthawks 6 at Buffalo Bandits 13 | Final |
| January 4, 2025 | W | Rochester Knighthawks 16 at Georgia Swarm 9 | Final |
| January 11, 2025 | L | Halifax Thunderbirds 19 at Rochester Knighthawks 18 | Final |
| January 18, 2025 | W | Rochester Knighthawks 10 at Halifax Thunderbirds 9 | Final |
| January 25, 2025 | L | Colorado Mammoth 11 at Rochester Knighthawks 10 | Final |
| February 1, 2025 | | Rochester Knighthawks at Philadelphia Wings | 8:00 p.m. |
| February 15, 2025 | | Rochester Knighthawks at Saskatchewan Rush | 8:00 p.m. |
| February 22, 2025 | | Rochester Knighthawks at Calgary Roughnecks | 9:00 p.m. |
| February 28, 2025 | | Albany FireWolves at Rochester Knighthawks | 7:00 p.m. |
| March 7, 2025 | | Rochester Knighthawks at Las Vegas Desert Dogs | 10:30 p.m. |
| March 15, 2025 | | Rochester Knighthawks at Ottawa Black Bears | 3:00 p.m. |
| March 29, 2025 | | San Diego Seals at Rochester Knighthawks | 7:00 p.m. |
| April 5, 2025 | | Ottawa Black Bears at Rochester Knighthawks | 7:00 p.m. |
| April 19, 2025 | | Toronto Rock at Rochester Knighthawks | 6:00 p.m. |
Rochester Knighthawks Schedule
