| | | |
| March 21, 2025 | W | Jacksonville Sharks 46 at Massachusetts Pirates 25 | Final |
| April 6, 2025 | W | Vegas Knight Hawks 19 at Jacksonville Sharks 36 | Final |
| April 12, 2025 | W | Jacksonville Sharks 28 at Tucson Sugar Skulls 21 | Final |
| April 19, 2025 | L | Massachusetts Pirates 42 at Jacksonville Sharks 35 | Final |
| April 26, 2025 | L | Jacksonville Sharks 41 at Quad City Steamwheelers 62 | Final |
| May 3, 2025 | W | Iowa Barnstormers 22 at Jacksonville Sharks 53 | Final |
| May 17, 2025 | W | Fishers Freight 27 at Jacksonville Sharks 59 | Final |
| May 24, 2025 | W | Jacksonville Sharks 46 at Fishers Freight 40 | Final |
| May 29, 2025 | L | Jacksonville Sharks 29 at Massachusetts Pirates 43 | Final |
| June 7, 2025 | W | Arizona Rattlers 48 at Jacksonville Sharks 54 | Final |
| June 13, 2025 | L | Jacksonville Sharks 43 at Green Bay Blizzard 75 | Final |
| June 21, 2025 | W | Quad City Steamwheelers 33 at Jacksonville Sharks 65 | Final |
| June 28, 2025 | W | Jacksonville Sharks 58 at Iowa Barnstormers 40 | Final |
| July 12, 2025 | L | San Antonio Gunslingers 46 at Jacksonville Sharks 31 | Final |
| July 19, 2025 | L | Jacksonville Sharks 38 at Tulsa Oilers 47 | Final |
| July 26, 2025 | W | Green Bay Blizzard 53 at Jacksonville Sharks 63 | Final |
| August 2, 2025 | L | Jacksonville Sharks 41 at Quad City Steamwheelers 57 (Playoff) | Final |
Jacksonville Sharks Schedule
