| | | |
| March 12, 2025 | L | Union Omaha 0 at Greenville Triumph SC 3 | Final |
| March 29, 2025 | W | Union Omaha 3 at South Georgia Tormenta FC 1 | Final |
| April 9, 2025 | L | Union Omaha 0 at Richmond Kickers 1 | Final |
| April 26, 2025 | L | Union Omaha 0 at New Mexico United 2 (Jagermeister Cup) | Final |
| May 3, 2025 | | Union Omaha at Forward Madison FC | 6:00 p.m. |
| May 10, 2025 | | Portland Hearts of Pine at Union Omaha | 8:00 p.m. |
| May 17, 2025 | | Union Omaha at Texoma FC | 8:00 p.m. |
| May 25, 2025 | | Union Omaha at FC Naples | 8:00 p.m. |
| June 7, 2025 | | Union Omaha at Forward Madison FC | 8:00 p.m. |
| June 12, 2025 | | Greenville Triumph SC at Union Omaha | 8:00 p.m. |
| June 15, 2025 | | Union Omaha at Portland Hearts of Pine | 6:00 p.m. |
| June 25, 2025 | | Richmond Kickers at Union Omaha | 8:00 p.m. |
| June 28, 2025 | | Union Omaha at San Antonio FC | 9:00 p.m. |
| July 5, 2025 | | Union Omaha at AV Alta FC | 11:00 p.m. |
| July 12, 2025 | | Union Omaha at Charlotte Independence | 7:00 p.m. |
| July 18, 2025 | | South Georgia Tormenta FC at Union Omaha | 8:00 p.m. |
| July 23, 2025 | | Texoma FC at Union Omaha | 8:00 p.m. |
| July 26, 2025 | | El Paso Locomotive FC at Union Omaha | 8:00 p.m. |
| August 2, 2025 | | Union Omaha at Spokane Velocity FC | 10:00 p.m. |
| August 6, 2025 | | Westchester SC at Union Omaha | 8:00 p.m. |
| August 9, 2025 | | Chattanooga Red Wolves SC at Union Omaha | 8:00 p.m. |
| August 17, 2025 | | Union Omaha at One Knoxville SC | 5:00 p.m. |
| August 20, 2025 | | Charlotte Independence at Union Omaha | 8:00 p.m. |
| August 23, 2025 | | AV Alta FC at Union Omaha | 8:00 p.m. |
| September 6, 2025 | | Texoma FC at Union Omaha | TBD |
| September 13, 2025 | | Union Omaha at Westchester SC | 7:00 p.m. |
| September 17, 2025 | | Spokane Velocity FC at Union Omaha | 8:00 p.m. |
| September 21, 2025 | | One Knoxville SC at Union Omaha | TBD |
| September 27, 2025 | | Union Omaha at Chattanooga Red Wolves SC | 7:00 p.m. |
| October 1, 2025 | | Portland Hearts of Pine at Union Omaha | 1:00 p.m. |
| October 5, 2025 | | FC Naples at Union Omaha | TBD |
| October 11, 2025 | | Union Omaha at Greenville Triumph SC | 7:00 p.m. |
| October 18, 2025 | | Forward Madison FC at Union Omaha | TBD |
| October 25, 2025 | | Spokane Velocity FC at Union Omaha | TBD |
Union Omaha Schedule
