| | | |
| March 7, 2025 | L | Tacoma Defiance 2 at Ventura County FC 3 | Final |
| March 14, 2025 | W | The Town FC 1 at Tacoma Defiance 3 | Final |
| April 6, 2025 | W | Vancouver Whitecaps FC 2 3 at Tacoma Defiance 5 | Final |
| April 10, 2025 | L | Tacoma Defiance 2 at LAFC2 3 | Final |
| April 23, 2025 | W | Tacoma Defiance 4 at Sporting Kansas City II 1 | Final |
| April 26, 2025 | W | St. Louis City SC 2 1 at Tacoma Defiance 4 | Final |
| May 2, 2025 | W | Tacoma Defiance 2 at Ventura County FC 1 | Final |
| May 14, 2025 | | Tacoma Defiance at Vancouver Whitecaps FC 2 | 9:00 p.m. |
| May 18, 2025 | | Tacoma Defiance at Portland Timbers 2 | 10:30 p.m. |
| May 25, 2025 | | North Texas SC at Tacoma Defiance | 10:00 p.m. |
| May 31, 2025 | | Minnesota United FC 2 at Tacoma Defiance | 10:00 p.m. |
| June 11, 2025 | | Tacoma Defiance at Real Monarchs | 9:30 p.m. |
| June 29, 2025 | | Tacoma Defiance at LAFC2 | 10:00 p.m. |
| July 3, 2025 | | The Town FC at Tacoma Defiance | 10:00 p.m. |
| July 17, 2025 | | Real Monarchs at Tacoma Defiance | 10:00 p.m. |
| July 20, 2025 | | Tacoma Defiance at Colorado Rapids 2 | 8:00 p.m. |
| July 27, 2025 | | Portland Timbers 2 at Tacoma Defiance | 10:00 p.m. |
| August 1, 2025 | | Ventura County FC at Tacoma Defiance | 10:00 p.m. |
| August 10, 2025 | | LAFC2 at Tacoma Defiance | 11:00 p.m. |
| August 15, 2025 | | Tacoma Defiance at Minnesota United FC 2 | 8:00 p.m. |
| August 24, 2025 | | Tacoma Defiance at Portland Timbers 2 | 11:00 p.m. |
| August 31, 2025 | | Tacoma Defiance at Real Monarchs | 9:30 p.m. |
| September 4, 2025 | | Vancouver Whitecaps FC 2 at Tacoma Defiance | 10:00 p.m. |
| September 12, 2025 | | Austin FC II at Tacoma Defiance | 10:00 p.m. |
| September 17, 2025 | | Tacoma Defiance at Houston Dynamo 2 | 8:00 p.m. |
| September 21, 2025 | | Colorado Rapids 2 at Tacoma Defiance | 10:00 p.m. |
| September 28, 2025 | | Tacoma Defiance at The Town FC | 8:00 p.m. |
| October 5, 2025 | | Houston Dynamo 2 at Tacoma Defiance | 4:00 p.m. |
Tacoma Defiance Schedule
