| | | |
| February 28, 2026 | W | Sporting Kansas City II 0 at St. Louis City SC 2 4 | Final |
| March 8, 2026 | W | Minnesota United FC 2 0 at St. Louis City SC 2 3 | Final |
| March 15, 2026 | W | St. Louis City SC 2 3 at Ventura County FC 2 | Final |
| March 22, 2026 | T | LAFC2 2 at St. Louis City SC 2 2 | Final (PK 3-4) |
| March 28, 2026 | W | St. Louis City SC 2 2 at Sporting Kansas City II 0 | Final |
| April 10, 2026 | T | Colorado Rapids 2 2 at St. Louis City SC 2 2 | Final (PK 3-5) |
| April 17, 2026 | W | St. Louis City SC 2 2 at Tacoma Defiance 1 | Final |
| April 26, 2026 | | Ventura County FC at St. Louis City SC 2 | 5:00 p.m. |
| May 2, 2026 | | St. Louis City SC 2 at Minnesota United FC 2 | 4:00 p.m. |
| May 9, 2026 | | Houston Dynamo 2 at St. Louis City SC 2 | 5:00 p.m. |
| May 17, 2026 | | St. Louis City SC 2 at Austin FC II | 8:30 p.m. |
| May 24, 2026 | | St. Louis City SC 2 at LAFC2 | 4:00 p.m. |
| May 30, 2026 | | North Texas SC at St. Louis City SC 2 | 5:00 p.m. |
| June 6, 2026 | | St. Louis City SC 2 at Colorado Rapids 2 | 8:00 p.m. |
| June 20, 2026 | | St. Louis City SC 2 at Minnesota United FC 2 | 4:00 p.m. |
| June 28, 2026 | | Austin FC II at St. Louis City SC 2 | 7:00 p.m. |
| July 4, 2026 | | St. Louis City SC 2 at North Texas SC | 8:45 p.m. |
| July 11, 2026 | | St. Louis City SC 2 at Houston Dynamo 2 | 7:00 p.m. |
| July 18, 2026 | | Portland Timbers 2 at St. Louis City SC 2 | 7:00 p.m. |
| July 26, 2026 | | Vancouver Whitecaps FC 2 at St. Louis City SC 2 | 7:00 p.m. |
| August 2, 2026 | | Real Monarchs at St. Louis City SC 2 | 7:00 p.m. |
| August 8, 2026 | | St. Louis City SC 2 at Colorado Rapids 2 | 8:00 p.m. |
| August 16, 2026 | | St. Louis City SC 2 at San Jose Earthquakes II | 12:00 p.m. |
| August 23, 2026 | | Houston Dynamo 2 at St. Louis City SC 2 | 7:00 p.m. |
| August 28, 2026 | | North Texas SC at St. Louis City SC 2 | 8:00 p.m. |
| September 5, 2026 | | St. Louis City SC 2 at Sporting Kansas City II | 8:00 p.m. |
| September 14, 2026 | | St. Louis City SC 2 at Portland Timbers 2 | 4:00 p.m. |
| September 20, 2026 | | Austin FC II at St. Louis City SC 2 | 5:00 p.m. |
St. Louis City SC 2 Schedule
