| | | |
| June 5, 2025 | T | Kingsport Axmen 10 at Pulaski River Turtles 10 (Pul wins SD) | Final |
| June 6, 2025 | L | Kingsport Axmen 15 at Pulaski River Turtles 6 | Final |
| June 7, 2025 | W | Kingsport Axmen 0 at Pulaski River Turtles 4 | Final |
| June 9, 2025 | W | Pulaski River Turtles 10 at Tri-State Coal Cats 5 | Final |
| June 10, 2025 | L | Pulaski River Turtles 1 at Tri-State Coal Cats 9 | Final |
| June 11, 2025 | T | Danville Otterbots 10 at Pulaski River Turtles 10 (Pul wins SD) | Final |
| June 12, 2025 | | Danville Otterbots 6 at Pulaski River Turtles 6 | Bot 9th |
| June 13, 2025 | L | Pulaski River Turtles 7 at Bristol State Liners 13 | Final |
| June 14, 2025 | W | Pulaski River Turtles 8 at Bristol State Liners 4 | Final |
| June 15, 2025 | L | Pulaski River Turtles 2 at Bristol State Liners 7 | Final (7) |
| June 17, 2025 | W | Tri-State Coal Cats 6 at Pulaski River Turtles 9 | Final |
| June 18, 2025 | W | Tri-State Coal Cats 2 at Pulaski River Turtles 3 | Final |
| June 19, 2025 | | Pulaski River Turtles 7 at Johnson City Doughboys 7 | Top 10th |
| June 20, 2025 | L | Pulaski River Turtles 2 at Johnson City Doughboys 4 | Final |
| June 21, 2025 | L | Pulaski River Turtles 4 at Greeneville Flyboys 5 | Final |
| June 22, 2025 | L | Pulaski River Turtles 5 at Greeneville Flyboys 6 | Final (7) |
| June 24, 2025 | W | Bluefield Ridge Runners 6 at Pulaski River Turtles 7 | Final |
| June 25, 2025 | W | Bluefield Ridge Runners 9 at Pulaski River Turtles 10 | Final |
| June 26, 2025 | L | Burlington Sock Puppets 11 at Pulaski River Turtles 5 | Final |
| June 27, 2025 | T | Burlington Sock Puppets 4 at Pulaski River Turtles 4 (Pul wins SD) | Final |
| June 28, 2025 | | Greeneville Flyboys at Pulaski River Turtles | Cancelled |
| June 29, 2025 | L | Greeneville Flyboys 11 at Pulaski River Turtles 5 | Final (7) |
| July 1, 2025 | | Pulaski River Turtles at Danville Otterbots | Ppd. |
| July 2, 2025 | W | Pulaski River Turtles 13 at Danville Otterbots 3 | Final (8) |
| July 3, 2025 | W | Pulaski River Turtles 8 at Danville Otterbots 6 | Final |
| July 4, 2025 | L | Elizabethton River Riders 8 at Pulaski River Turtles 1 | Final |
| July 5, 2025 | L | Elizabethton River Riders 16 at Pulaski River Turtles 6 | Final |
| July 6, 2025 | W | Elizabethton River Riders 4 at Pulaski River Turtles 5 | Final (7) |
| July 8, 2025 | W | Pulaski River Turtles 9 at Bluefield Ridge Runners 8 | Final |
| July 9, 2025 | L | Pulaski River Turtles 2 at Bluefield Ridge Runners 10 | Final |
| July 10, 2025 | L | Tri-State Coal Cats 19 at Pulaski River Turtles 10 | Final |
| July 11, 2025 | W | Tri-State Coal Cats 14 at Pulaski River Turtles 15 | Final |
| July 12, 2025 | W | Pulaski River Turtles 8 at Elizabethton River Riders 7 | Final |
| July 13, 2025 | T | Pulaski River Turtles 3 at Elizabethton River Riders 3 (Pul wins SD) | Final (7) |
| July 15, 2025 | | Johnson City Doughboys at Pulaski River Turtles | Cancelled |
| July 16, 2025 | W | Johnson City Doughboys 1 at Pulaski River Turtles 3 | Final |
| July 17, 2025 | | Pulaski River Turtles at Kingsport Axmen | Cancelled |
| July 18, 2025 | L | Pulaski River Turtles 0 at Kingsport Axmen 14 | Final |
| July 19, 2025 | L | Bristol State Liners 5 at Pulaski River Turtles 2 | Final |
| July 20, 2025 | W | Bristol State Liners 1 at Pulaski River Turtles 2 | Final (7) |
| July 22, 2025 | T | Pulaski River Turtles 1 at Burlington Sock Puppets 1 (Bur wins SD) | Final |
| July 23, 2025 | L | Pulaski River Turtles 2 at Burlington Sock Puppets 12 | Final (7) |
| July 24, 2025 | L | Pulaski River Turtles 6 at Danville Otterbots 7 (Game 1) | Final (7) |
| July 24, 2025 | W | Pulaski River Turtles 2 at Danville Otterbots 0 (Game 2) | Final (7) |
| July 25, 2025 | W | Pulaski River Turtles 5 at Danville Otterbots 4 | Final |
| July 26, 2025 | T | Pulaski River Turtles 7 at Bluefield Ridge Runners 7 | Final |
| July 27, 2025 | L | Pulaski River Turtles 4 at Bluefield Ridge Runners 8 | Final (7) |
| July 29, 2025 | L | Bluefield Ridge Runners 6 at Pulaski River Turtles 3 | Final |
| July 30, 2025 | W | Bluefield Ridge Runners 8 at Pulaski River Turtles 9 | Final |
Pulaski River Turtles Schedule
