| | | |
| March 8, 2025 | W | Sporting Kansas City II 1 at Houston Dynamo 2 3 | Final |
| March 23, 2025 | W | North Texas SC 1 at Houston Dynamo 2 5 | Final |
| March 26, 2025 | L | Houston Dynamo 2 0 at Ventura County FC 1 | Final |
| March 30, 2025 | L | Minnesota United FC 2 3 at Houston Dynamo 2 1 | Final |
| April 6, 2025 | W | LAFC2 0 at Houston Dynamo 2 1 | Final |
| April 23, 2025 | W | Houston Dynamo 2 3 at Vancouver Whitecaps FC 2 1 | Final |
| April 27, 2025 | W | Austin FC II 1 at Houston Dynamo 2 2 | Final |
| May 4, 2025 | L | Houston Dynamo 2 1 at St. Louis City SC 2 3 | Final |
| May 9, 2025 | T | Colorado Rapids 2 0 at Houston Dynamo 2 0 | Final (PK 4-5) |
| May 18, 2025 | L | Houston Dynamo 2 1 at North Texas SC 2 | Final |
| May 30, 2025 | T | Houston Dynamo 2 1 at Austin FC II 1 | Final (PK 4-3) |
| June 7, 2025 | T | Sporting Kansas City II 1 at Houston Dynamo 2 1 | Final (PK 4-5) |
| June 12, 2025 | L | Houston Dynamo 2 1 at Minnesota United FC 2 4 | Final |
| June 15, 2025 | T | Houston Dynamo 2 1 at Real Monarchs 1 | Final (PK 4-5) |
| June 28, 2025 | L | Houston Dynamo 2 0 at St. Louis City SC 2 1 | Final |
| July 6, 2025 | L | Houston Dynamo 2 1 at Colorado Rapids 2 2 | Final |
| July 13, 2025 | W | North Texas SC 0 at Houston Dynamo 2 3 | Final |
| July 17, 2025 | L | Vancouver Whitecaps FC 2 4 at Houston Dynamo 2 2 | Final |
| July 25, 2025 | L | Houston Dynamo 2 0 at Austin FC II 2 | Final |
| August 2, 2025 | T | Houston Dynamo 2 2 at Minnesota United FC 2 2 | Final (PK 3-4) |
| August 9, 2025 | L | Real Monarchs 4 at Houston Dynamo 2 0 | Final |
| August 17, 2025 | W | Houston Dynamo 2 3 at Sporting Kansas City II 1 | Final |
| August 22, 2025 | L | The Town FC 1 at Houston Dynamo 2 0 | Final |
| August 31, 2025 | T | St. Louis City SC 2 2 at Houston Dynamo 2 2 | Final (PK 3-4) |
| September 14, 2025 | W | Houston Dynamo 2 1 at Colorado Rapids 2 0 | Final |
| September 17, 2025 | L | Tacoma Defiance 2 at Houston Dynamo 2 1 | Final |
| September 21, 2025 | | Portland Timbers 2 at Houston Dynamo 2 | 8:00 p.m. |
| October 5, 2025 | | Houston Dynamo 2 at Tacoma Defiance | 4:00 p.m. |
Houston Dynamo 2 Schedule
