| | | |
| March 7, 2025 | W | Colorado Rapids 2 2 at Austin FC II 4 | Final |
| March 14, 2025 | L | Minnesota United FC 2 1 at Austin FC II 0 | Final |
| March 30, 2025 | T | Austin FC II 0 at St. Louis City SC 2 0 | Final (PK 4-5) |
| April 11, 2025 | T | North Texas SC 1 at Austin FC II 1 | Final (PK 4-2) |
| April 19, 2025 | L | Austin FC II 0 at Vancouver Whitecaps FC 2 4 | Final |
| April 27, 2025 | L | Austin FC II 1 at Houston Dynamo 2 2 | Final |
| May 2, 2025 | | Sporting Kansas City II at Austin FC II | 8:30 p.m. |
| May 13, 2025 | | Austin FC II at Ventura County FC | 10:00 p.m. |
| May 23, 2025 | | Minnesota United FC 2 at Austin FC II | 8:30 p.m. |
| May 30, 2025 | | Houston Dynamo 2 at Austin FC II | 8:30 p.m. |
| June 8, 2025 | | Austin FC II at Colorado Rapids 2 | 8:00 p.m. |
| June 14, 2025 | | Austin FC II at North Texas SC | 8:45 p.m. |
| June 20, 2025 | | Austin FC II at Portland Timbers 2 | 11:00 p.m. |
| June 27, 2025 | | Sporting Kansas City II at Austin FC II | 8:30 p.m. |
| July 4, 2025 | | LAFC2 at Austin FC II | 8:30 p.m. |
| July 11, 2025 | | St. Louis City SC 2 at Austin FC II | 8:30 p.m. |
| July 20, 2025 | | Austin FC II at Real Monarchs | 9:30 p.m. |
| July 25, 2025 | | Houston Dynamo 2 at Austin FC II | 8:30 p.m. |
| August 3, 2025 | | Austin FC II at Sporting Kansas City II | 1:00 p.m. |
| August 8, 2025 | | North Texas SC at Austin FC II | 8:30 p.m. |
| August 15, 2025 | | Austin FC II at Colorado Rapids 2 | 8:00 p.m. |
| August 22, 2025 | | Ventura County FC at Austin FC II | 8:30 p.m. |
| August 29, 2025 | | Austin FC II at LAFC2 | 10:00 p.m. |
| September 3, 2025 | | Austin FC II at Minnesota United FC 2 | 4:00 p.m. |
| September 12, 2025 | | Austin FC II at Tacoma Defiance | 10:00 p.m. |
| September 21, 2025 | | St. Louis City SC 2 at Austin FC II | 8:30 p.m. |
| September 26, 2025 | | Portland Timbers 2 at Austin FC II | 8:30 p.m. |
| October 5, 2025 | | Austin FC II at The Town FC | 4:00 p.m. |
Austin FC II Schedule
