| | | |
| March 14, 2025 | W | Washington Spirit 2 at Houston Dash 1 | Final |
| March 22, 2025 | L | Kansas City Current 2 at Washington Spirit 0 | Final |
| March 28, 2025 | W | Bay FC 0 at Washington Spirit 2 | Final |
| April 12, 2025 | W | Washington Spirit 2 at Racing Louisville FC 0 | Final |
| April 19, 2025 | W | Washington Spirit 1 at Orlando Pride 0 | Final |
| April 26, 2025 | L | NJ/NY Gotham FC 3 at Washington Spirit 0 | Final |
| May 2, 2025 | L | Angel City FC 4 at Washington Spirit 3 | Final |
| May 10, 2025 | W | Washington Spirit 3 at Chicago Stars FC 2 | Final |
| May 17, 2025 | T | Utah Royals FC 3 at Washington Spirit 3 | Final |
| May 23, 2025 | W | Washington Spirit 2 at Seattle Reign FC 1 | Final |
| June 8, 2025 | W | North Carolina Courage 1 at Washington Spirit 3 | Final |
| June 15, 2025 | L | Washington Spirit 0 at Portland Thorns FC 2 | Final |
| June 22, 2025 | T | Washington Spirit 0 at San Diego Wave FC 0 | Final |
| August 3, 2025 | W | Portland Thorns FC 1 at Washington Spirit 2 | Final |
| August 9, 2025 | | Washington Spirit at NJ/NY Gotham FC | 12:00 p.m. |
| August 15, 2025 | | Racing Louisville FC at Washington Spirit | 8:00 p.m. |
| August 23, 2025 | | Washington Spirit at Bay FC | 4:00 p.m. |
| August 31, 2025 | | Chicago Stars FC at Washington Spirit | 4:00 p.m. |
| September 7, 2025 | | Seattle Reign FC at Washington Spirit | 4:00 p.m. |
| September 13, 2025 | | Washington Spirit at Kansas City Current | 7:30 p.m. |
| September 18, 2025 | | Washington Spirit at Angel City FC | 10:30 p.m. |
| September 28, 2025 | | Houston Dash at Washington Spirit | 1:00 p.m. |
| October 5, 2025 | | San Diego Wave FC at Washington Spirit | 1:00 p.m. |
| October 11, 2025 | | Washington Spirit at North Carolina Courage | 7:30 p.m. |
| October 18, 2025 | | Orlando Pride at Washington Spirit | 12:30 p.m. |
| November 2, 2025 | | Washington Spirit at Utah Royals FC | TBD |
Washington Spirit Schedule
