| | | |
| November 7, 2025 | L | Oklahoma City Blue 116 at Rio Grande Valley Vipers 114 | Final |
| November 13, 2025 | L | Texas Legends 119 at Rio Grande Valley Vipers 110 | Final |
| November 15, 2025 | W | Texas Legends 126 at Rio Grande Valley Vipers 133 | Final |
| November 19, 2025 | W | Rio Grande Valley Vipers 130 at Memphis Hustle 125 | Final |
| November 21, 2025 | W | Rio Grande Valley Vipers 110 at Memphis Hustle 107 | Final |
| November 23, 2025 | L | Rio Grande Valley Vipers 123 at Austin Spurs 130 | Final |
| November 24, 2025 | L | Rio Grande Valley Vipers 100 at Austin Spurs 118 | Final |
| November 29, 2025 | L | Osceola Magic 134 at Rio Grande Valley Vipers 125 | Final |
| December 1, 2025 | W | Osceola Magic 108 at Rio Grande Valley Vipers 113 | Final |
| December 4, 2025 | W | Mexico City Capitanes 107 at Rio Grande Valley Vipers 125 | Final |
| December 6, 2025 | L | Mexico City Capitanes 118 at Rio Grande Valley Vipers 113 | Final |
| December 12, 2025 | W | Rio Grande Valley Vipers 120 at Oklahoma City Blue 119 | Final |
| December 14, 2025 | W | Rio Grande Valley Vipers 133 at Laketown Squadron 127 | Final |
| December 16, 2025 | L | Rio Grande Valley Vipers 111 at Laketown Squadron 142 | Final |
| December 20, 2025 | W | Westchester Knicks 101 at Rio Grande Valley Vipers 127 (Orlando) | Final |
| December 22, 2025 | W | Rio Grande Valley Vipers 118 at Wisconsin Herd 103 (Orlando) | Final |
| December 31, 2025 | L | Rio Grande Valley Vipers 114 at Sioux Falls Skyforce 121 | Final |
| January 3, 2026 | W | Valley Suns 115 at Rio Grande Valley Vipers 130 | Final |
| January 5, 2026 | L | Rio Grande Valley Vipers 104 at Stockton Kings 110 | Final |
| January 7, 2026 | L | Rio Grande Valley Vipers 115 at Stockton Kings 128 | Final |
| January 9, 2026 | W | Greensboro Swarm 115 at Rio Grande Valley Vipers 120 | Final |
| January 10, 2026 | W | Greensboro Swarm 119 at Rio Grande Valley Vipers 131 | Final |
| January 13, 2026 | L | Rio Grande Valley Vipers 97 at Mexico City Capitanes 122 | Final |
| January 14, 2026 | L | Rio Grande Valley Vipers 102 at Mexico City Capitanes 139 | Final |
| January 17, 2026 | W | Coachella Valley Lakers 121 at Rio Grande Valley Vipers 126 | Final |
| January 20, 2026 | W | Coachella Valley Lakers 119 at Rio Grande Valley Vipers 126 | Final |
| January 22, 2026 | W | Rip City Remix 99 at Rio Grande Valley Vipers 108 | Final |
| January 24, 2026 | W | Rip City Remix 112 at Rio Grande Valley Vipers 134 | Final |
| January 26, 2026 | L | Rio Grande Valley Vipers 129 at Iowa Wolves 136 | Final |
| February 1, 2026 | W | Rio Grande Valley Vipers 109 at Oklahoma City Blue 94 | Final |
| February 4, 2026 | W | Valley Suns 116 at Rio Grande Valley Vipers 130 | Final |
| February 7, 2026 | W | Austin Spurs 111 at Rio Grande Valley Vipers 112 | Final |
| February 12, 2026 | W | Rio Grande Valley Vipers 121 at Texas Legends 120 | Final (OT) |
| February 19, 2026 | L | Rio Grande Valley Vipers 106 at Sioux Falls Skyforce 123 | Final |
| February 22, 2026 | W | Rio Grande Valley Vipers 117 at Iowa Wolves 115 | Final |
| February 24, 2026 | L | San Diego Clippers 123 at Rio Grande Valley Vipers 122 | Final |
| February 25, 2026 | W | San Diego Clippers 102 at Rio Grande Valley Vipers 109 | Final |
| February 27, 2026 | W | Noblesville Boom 133 at Rio Grande Valley Vipers 135 | Final (OT) |
| March 4, 2026 | W | Rio Grande Valley Vipers 123 at Raptors 905 121 | Final |
| March 6, 2026 | L | Rio Grande Valley Vipers 120 at Raptors 905 128 | Final |
| March 8, 2026 | W | Rio Grande Valley Vipers 137 at Windy City Bulls 100 | Final |
| March 10, 2026 | L | Austin Spurs 94 at Rio Grande Valley Vipers 86 | Final |
| March 13, 2026 | W | Salt Lake City Stars 101 at Rio Grande Valley Vipers 128 | Final |
| March 14, 2026 | W | Salt Lake City Stars 105 at Rio Grande Valley Vipers 116 | Final |
| March 17, 2026 | L | Rio Grande Valley Vipers 104 at Texas Legends 108 | Final |
| March 19, 2026 | W | Rio Grande Valley Vipers 108 at Santa Cruz Warriors 99 | Final |
| March 21, 2026 | L | Rio Grande Valley Vipers 115 at Santa Cruz Warriors 123 | Final |
| March 24, 2026 | W | Rio Grande Valley Vipers 120 at Oklahoma City Blue 105 | Final |
| March 26, 2026 | W | Memphis Hustle 107 at Rio Grande Valley Vipers 121 | Final |
| March 28, 2026 | L | Memphis Hustle 120 at Rio Grande Valley Vipers 118 | Final |
| March 31, 2026 | W | Austin Spurs 106 at Rio Grande Valley Vipers 117 (Playoff) | Final |
| April 3, 2026 | L | Rio Grande Valley Vipers 97 at Coachella Valley Lakers 112 (Playoff) | Final |
Rio Grande Valley Vipers Schedule
