| | | |
| March 2, 2026 | L | Huntsville City Football Club 3 at Orlando City B 1 | Final |
| March 8, 2026 | W | Chicago Fire FC II 1 at Orlando City B 2 | Final |
| March 14, 2026 | W | Orlando City B 3 at Carolina Core FC 2 | Final |
| March 22, 2026 | | Inter Miami CF II at Orlando City B | 6:00 p.m. |
| April 11, 2026 | | Orlando City B at Chattanooga FC | 6:00 p.m. |
| April 19, 2026 | | Carolina Core FC at Orlando City B | 6:00 p.m. |
| April 26, 2026 | | Orlando City B at Chicago Fire FC II | 3:00 p.m. |
| May 3, 2026 | | Crown Legacy FC at Orlando City B | 6:00 p.m. |
| May 10, 2026 | | Orlando City B at Atlanta United 2 | 6:00 p.m. |
| May 17, 2026 | | Orlando City B at New England Revolution II | 1:00 p.m. |
| May 24, 2026 | | Inter Miami CF II at Orlando City B | 6:00 p.m. |
| June 7, 2026 | | Carolina Core FC at Orlando City B | 6:00 p.m. |
| June 13, 2026 | | Orlando City B at Huntsville City Football Club | 7:00 p.m. |
| June 21, 2026 | | Philadelphia Union II at Orlando City B | 6:00 p.m. |
| June 28, 2026 | | Columbus Crew 2 at Orlando City B | 6:00 p.m. |
| July 5, 2026 | | Orlando City B at Crown Legacy FC | 6:00 p.m. |
| July 12, 2026 | | Orlando City B at FC Cincinnati 2 | 5:00 p.m. |
| July 18, 2026 | | Orlando City B at Chattanooga FC | 6:00 p.m. |
| July 26, 2026 | | Huntsville City Football Club at Orlando City B | 6:00 p.m. |
| August 1, 2026 | | Orlando City B at Connecticut United FC | 6:00 p.m. |
| August 12, 2026 | | Orlando City B at New York City FC II | 4:00 p.m. |
| August 16, 2026 | | Orlando City B at Chicago Fire FC II | 3:00 p.m. |
| August 19, 2026 | | Chattanooga FC at Orlando City B | 6:00 p.m. |
| August 22, 2026 | | Orlando City B at Atlanta United 2 | 6:00 p.m. |
| August 30, 2026 | | Crown Legacy FC at Orlando City B | 6:00 p.m. |
| September 4, 2026 | | Orlando City B at Inter Miami CF II | 6:00 p.m. |
| September 13, 2026 | | Toronto FC II at Orlando City B | 6:00 p.m. |
| September 20, 2026 | | Atlanta United 2 at Orlando City B | 1:00 p.m. |
Orlando City B Schedule
