| | | |
| March 9, 2025 | W | Columbus Crew 2 1 at Orlando City B 2 | Final |
| March 16, 2025 | T | Atlanta United 2 2 at Orlando City B 2 | Final (PK 1-4) |
| March 26, 2025 | W | Orlando City B 1 at Crown Legacy FC 0 | Final |
| April 5, 2025 | L | Orlando City B 0 at Chattanooga FC 2 | Final |
| April 9, 2025 | W | New England Revolution II 0 at Orlando City B 1 | Final |
| April 15, 2025 | L | Orlando City B 2 at Inter Miami CF II 3 | Final |
| April 27, 2025 | L | New York Red Bulls II 4 at Orlando City B 2 | Final |
| May 2, 2025 | L | Orlando City B 1 at Chicago Fire FC II 5 | Final |
| May 17, 2025 | W | Inter Miami CF II 0 at Orlando City B 3 | Final |
| May 25, 2025 | L | Orlando City B 0 at Atlanta United 2 3 | Final |
| June 1, 2025 | W | Chattanooga FC 1 at Orlando City B 2 | Final |
| June 6, 2025 | W | Orlando City B 3 at Huntsville City Football Club 2 | Final |
| June 11, 2025 | L | Orlando City B 0 at Carolina Core FC 1 | Final |
| June 25, 2025 | L | Toronto FC II 2 at Orlando City B 1 | Final |
| June 30, 2025 | T | Orlando City B 1 at Philadelphia Union II 1 | Final (PK 2-4) |
| July 6, 2025 | T | Orlando City B 2 at Crown Legacy FC 2 | Final (PK 3-1) |
| July 13, 2025 | W | Carolina Core FC 1 at Orlando City B 3 | Final |
| July 20, 2025 | L | Orlando City B 0 at New England Revolution II 3 | Final |
| July 25, 2025 | L | Orlando City B 0 at Huntsville City Football Club 4 | Final |
| August 3, 2025 | L | New York City FC II 4 at Orlando City B 1 | Final |
| August 9, 2025 | T | Chattanooga FC 2 at Orlando City B 2 | Final (PK 4-2) |
| August 17, 2025 | L | Orlando City B 1 at Columbus Crew 2 2 | Final |
| August 23, 2025 | W | Atlanta United 2 1 at Orlando City B 2 | Final |
| August 31, 2025 | W | Crown Legacy FC 0 at Orlando City B 1 | Final |
| September 14, 2025 | T | Orlando City B 1 at Inter Miami CF II 1 | Final (PK 5-3) |
| September 21, 2025 | L | Huntsville City Football Club 1 at Orlando City B 0 | Final |
| September 27, 2025 | | Orlando City B at Carolina Core FC | 7:30 p.m. |
| October 5, 2025 | | FC Cincinnati 2 at Orlando City B | 1:00 p.m. |
Orlando City B Schedule
