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| March 7, 2026 | | One Knoxville SC at Westchester SC | 7:00 p.m. |
| March 14, 2026 | | Forward Madison FC at One Knoxville SC | 6:00 p.m. |
| March 21, 2026 | | Corpus Christi FC at One Knoxville SC | 4:00 p.m. |
| March 27, 2026 | | Richmond Kickers at One Knoxville SC | 6:00 p.m. |
| April 4, 2026 | | One Knoxville SC at Sarasota Paradise | 6:30 p.m. |
| April 11, 2026 | | One Knoxville SC at Portland Hearts of Pine | 5:00 p.m. |
| April 18, 2026 | | Charlotte Independence at One Knoxville SC | 6:00 p.m. |
| April 25, 2026 | | One Knoxville SC at FC Tulsa (USL Cup) | 7:00 p.m. |
| May 2, 2026 | | One Knoxville SC at FC Naples | 6:00 p.m. |
| May 9, 2026 | | One Knoxville SC at South Georgia Tormenta FC | 6:30 p.m. |
| May 13, 2026 | | Union Omaha at One Knoxville SC | 6:00 p.m. |
| May 16, 2026 | | San Antonio FC at One Knoxville SC (USL Cup) | 6:00 p.m. |
| May 23, 2026 | | One Knoxville SC at Corpus Christi FC | 7:30 p.m. |
| May 29, 2026 | | Greenville Triumph SC at One Knoxville SC | 5:30 p.m. |
| June 10, 2026 | | Chattanooga Red Wolves SC at One Knoxville SC (USL Cup) | 6:00 p.m. |
| June 13, 2026 | | New York Cosmos at One Knoxville SC | 6:00 p.m. |
| June 20, 2026 | | One Knoxville SC at Spokane Velocity FC | 8:00 p.m. |
| July 2, 2026 | | One Knoxville SC at Athletic Club Boise | 8:30 p.m. |
| July 11, 2026 | | One Knoxville SC at Corpus Christi FC (USL Cup) | 7:30 p.m. |
| July 15, 2026 | | Fort Wayne FC at One Knoxville SC | 5:30 p.m. |
| July 18, 2026 | | AV Alta FC at One Knoxville SC | 6:30 p.m. |
| July 25, 2026 | | One Knoxville SC at Chattanooga Red Wolves SC | 6:00 p.m. |
| August 1, 2026 | | One Knoxville SC at Charlotte Independence | 6:00 p.m. |
| August 8, 2026 | | Sarasota Paradise at One Knoxville SC | 6:30 p.m. |
| August 15, 2026 | | One Knoxville SC at Greenville Triumph SC | 6:00 p.m. |
| August 19, 2026 | | Portland Hearts of Pine at One Knoxville SC | 6:00 p.m. |
| August 22, 2026 | | Athletic Club Boise at One Knoxville SC | 6:30 p.m. |
| September 2, 2026 | | One Knoxville SC at Forward Madison FC | 7:00 p.m. |
| September 5, 2026 | | One Knoxville SC at AV Alta FC | 9:30 p.m. |
| September 11, 2026 | | FC Naples at One Knoxville SC | 7:30 p.m. |
| September 16, 2026 | | One Knoxville SC at Fort Wayne FC | 6:30 p.m. |
| September 20, 2026 | | One Knoxville SC at Union Omaha | 4:00 p.m. |
| September 27, 2026 | | Westchester SC at One Knoxville SC | 2:00 p.m. |
| October 3, 2026 | | One Knoxville SC at Richmond Kickers | 5:00 p.m. |
| October 7, 2026 | | Chattanooga Red Wolves SC at One Knoxville SC | 6:00 p.m. |
| October 11, 2026 | | Spokane Velocity FC at One Knoxville SC | 2:00 p.m. |
| October 17, 2026 | | One Knoxville SC at New York Cosmos | 3:00 p.m. |
| October 23, 2026 | | South Georgia Tormenta FC at One Knoxville SC | 6:00 p.m. |
One Knoxville SC Schedule
