| | | |
| March 7, 2026 | W | One Knoxville SC 3 at Westchester SC 2 | Final |
| March 14, 2026 | L | Forward Madison FC 2 at One Knoxville SC 1 | Final |
| March 24, 2026 | T | Corpus Christi FC 0 at One Knoxville SC 0 | Final |
| March 27, 2026 | W | Richmond Kickers 0 at One Knoxville SC 1 | Final |
| April 4, 2026 | W | One Knoxville SC 2 at Sarasota Paradise 1 | Final |
| April 11, 2026 | T | One Knoxville SC 0 at Portland Hearts of Pine 0 | Final |
| April 18, 2026 | W | Charlotte Independence 1 at One Knoxville SC 2 | Final |
| April 25, 2026 | T | One Knoxville SC 1 at FC Tulsa 1 (USL Cup) | Final (PK 3-2) |
| May 2, 2026 | W | One Knoxville SC 3 at FC Naples 0 | Final |
| May 13, 2026 | L | Union Omaha 2 at One Knoxville SC 1 | Final |
| May 16, 2026 | L | San Antonio FC 1 at One Knoxville SC 0 (USL Cup) | Final |
| May 23, 2026 | T | One Knoxville SC 2 at Corpus Christi FC 2 | Final |
| May 29, 2026 | W | Greenville Triumph SC 0 at One Knoxville SC 2 | Final |
| June 10, 2026 | T | Chattanooga Red Wolves SC 1 at One Knoxville SC 1 (USL Cup) | Final (PK 4-5) |
| June 13, 2026 | W | New York Cosmos 1 at One Knoxville SC 3 | Final |
| June 20, 2026 | W | One Knoxville SC 4 at Spokane Velocity FC 0 | Final |
| July 2, 2026 | W | One Knoxville SC 2 at Athletic Club Boise 1 | Final |
| July 11, 2026 | T | One Knoxville SC 1 at Corpus Christi FC 1 (USL Cup) | Final |
| July 11, 2026 | T | One Knoxville SC 1 at Corpus Christi FC 1 (USL Cup) | Final |
| July 15, 2026 | W | Fort Wayne FC 1 at One Knoxville SC 2 | Final |
| July 18, 2026 | L | AV Alta FC 3 at One Knoxville SC 2 | Final |
| July 25, 2026 | L | One Knoxville SC 0 at Chattanooga Red Wolves SC 1 | Final |
| August 1, 2026 | L | One Knoxville SC 1 at Charlotte Independence 3 | Final |
| August 8, 2026 | W | Sarasota Paradise 1 at One Knoxville SC 2 | Final |
| August 15, 2026 | T | One Knoxville SC 1 at Greenville Triumph SC 1 | Final |
| August 19, 2026 | T | Portland Hearts of Pine 1 at One Knoxville SC 1 | Final |
| August 22, 2026 | T | Athletic Club Boise 2 at One Knoxville SC 2 | Final |
| September 2, 2026 | T | One Knoxville SC 1 at Forward Madison FC 1 | Final |
| September 5, 2026 | W | One Knoxville SC 2 at AV Alta FC 1 | Final |
| September 11, 2026 | T | FC Naples 0 at One Knoxville SC 0 | Final |
| September 16, 2026 | W | One Knoxville SC 3 at Fort Wayne FC 2 | Final |
| September 19, 2026 | L | One Knoxville SC 1 at Union Omaha 2 | Final |
| September 27, 2026 | T | Westchester SC 0 at One Knoxville SC 0 | Final |
| October 3, 2026 | L | One Knoxville SC 2 at Richmond Kickers 3 | Final |
| October 7, 2026 | W | Chattanooga Red Wolves SC 0 at One Knoxville SC 3 | Final |
| October 11, 2026 | | Spokane Velocity FC at One Knoxville SC | 3:00 p.m. |
| October 17, 2026 | | One Knoxville SC at New York Cosmos | 4:00 p.m. |
One Knoxville SC Schedule
