| | | |
| March 1, 2026 | L | Columbus Crew 2 2 at Red Bull New York II 4 | Final |
| March 8, 2026 | T | New York City FC II 0 at Columbus Crew 2 0 | Final (PK 4-5) |
| March 15, 2026 | W | Toronto FC II 2 at Columbus Crew 2 3 | Final |
| March 21, 2026 | W | FC Cincinnati 2 0 at Columbus Crew 2 2 | Final |
| March 28, 2026 | L | Columbus Crew 2 0 at Chattanooga FC 2 | Final |
| April 11, 2026 | W | Columbus Crew 2 3 at Connecticut United FC 1 | Final |
| April 18, 2026 | W | New England Revolution II 1 at Columbus Crew 2 2 | Final |
| April 26, 2026 | L | Columbus Crew 2 1 at Red Bull New York II 4 | Final |
| May 3, 2026 | W | Inter Miami CF II 1 at Columbus Crew 2 3 | Final |
| May 10, 2026 | L | Columbus Crew 2 1 at FC Cincinnati 2 2 | Final |
| May 17, 2026 | T | Columbus Crew 2 1 at Philadelphia Union II 1 | Final (PK 8-7) |
| May 24, 2026 | | Toronto FC II at Columbus Crew 2 | 8:00 p.m. |
| May 30, 2026 | | Columbus Crew 2 at New York City FC II | 7:00 p.m. |
| June 8, 2026 | | Connecticut United FC at Columbus Crew 2 | 3:00 p.m. |
| June 14, 2026 | | Columbus Crew 2 at FC Cincinnati 2 | 6:00 p.m. |
| June 21, 2026 | | Atlanta United 2 at Columbus Crew 2 | 6:00 p.m. |
| June 28, 2026 | | Columbus Crew 2 at Orlando City B | 7:00 p.m. |
| July 15, 2026 | | Columbus Crew 2 at New England Revolution II | 7:00 p.m. |
| July 18, 2026 | | Philadelphia Union II at Columbus Crew 2 | 6:00 p.m. |
| July 26, 2026 | | Columbus Crew 2 at Connecticut United FC | 6:00 p.m. |
| July 31, 2026 | | Columbus Crew 2 at Toronto FC II | 7:00 p.m. |
| August 8, 2026 | | Red Bull New York II at Columbus Crew 2 | 6:00 p.m. |
| August 15, 2026 | | New York City FC II at Columbus Crew 2 | 6:00 p.m. |
| August 23, 2026 | | Columbus Crew 2 at Huntsville City FC | 7:00 p.m. |
| August 30, 2026 | | Chicago Fire FC II at Columbus Crew 2 | 1:00 p.m. |
| September 4, 2026 | | Columbus Crew 2 at Philadelphia Union II | 7:00 p.m. |
| September 13, 2026 | | New England Revolution II at Columbus Crew 2 | 6:00 p.m. |
| September 20, 2026 | | Carolina Core FC at Columbus Crew 2 | 2:00 p.m. |
Columbus Crew 2 Schedule
