| | | |
| August 30, 2025 | T | Carolina Ascent FC 2 at Fort Lauderdale United FC 2 | Final |
| September 6, 2025 | T | Tampa Bay Sun FC 2 at Carolina Ascent FC 2 | Final |
| September 13, 2025 | T | Carolina Ascent FC 2 at Sporting Club Jacksonville 2 | Final |
| September 24, 2025 | L | Spokane Zephyr FC 1 at Carolina Ascent FC 0 | Final |
| September 28, 2025 | L | Sporting Club Jacksonville 4 at Carolina Ascent FC 3 | Final |
| October 4, 2025 | W | DC Power FC 2 at Carolina Ascent FC 3 | Final |
| October 10, 2025 | T | Carolina Ascent FC 1 at Lexington Sporting Club 1 | Final |
| October 18, 2025 | W | Carolina Ascent FC 2 at Brooklyn FC 1 | Final |
| October 30, 2025 | W | Carolina Ascent FC 2 at Spokane Zephyr FC 1 | Final |
| November 8, 2025 | | Fort Lauderdale United FC at Carolina Ascent FC | 7:00 p.m. |
| November 21, 2025 | | Carolina Ascent FC at DC Power FC | 7:00 p.m. |
| December 6, 2025 | | Brooklyn FC at Carolina Ascent FC | 2:00 p.m. |
| December 13, 2025 | | Carolina Ascent FC at Dallas Trinity FC | 3:30 p.m. |
| December 20, 2025 | | DC Power FC at Carolina Ascent FC | 2:00 p.m. |
| January 31, 2026 | | Carolina Ascent FC at Sporting Club Jacksonville | 7:30 p.m. |
| February 7, 2026 | | Lexington Sporting Club at Carolina Ascent FC | 2:00 p.m. |
| February 14, 2026 | | Spokane Zephyr FC at Carolina Ascent FC | 2:00 p.m. |
| February 21, 2026 | | Dallas Trinity FC at Carolina Ascent FC | 2:00 p.m. |
| March 13, 2026 | | Tampa Bay Sun FC at Carolina Ascent FC | 7:00 p.m. |
| March 17, 2026 | | Carolina Ascent FC at DC Power FC | 7:00 p.m. |
| March 22, 2026 | | Carolina Ascent FC at Brooklyn FC | 3:00 p.m. |
| March 28, 2026 | | Fort Lauderdale United FC at Carolina Ascent FC | 7:00 p.m. |
| April 5, 2026 | | Carolina Ascent FC at Tampa Bay Sun FC | 5:00 p.m. |
| April 11, 2026 | | Lexington Sporting Club at Carolina Ascent FC | 7:00 p.m. |
| April 19, 2026 | | Carolina Ascent FC at Dallas Trinity FC | 5:00 p.m. |
| April 25, 2026 | | Carolina Ascent FC at Tampa Bay Sun FC | 7:30 p.m. |
| May 9, 2026 | | Carolina Ascent FC at Fort Lauderdale United FC | 7:30 p.m. |
| May 16, 2026 | | Sporting Club Jacksonville at Carolina Ascent FC | 7:00 p.m. |
Carolina Ascent FC Schedule
