| | | |
| March 7, 2025 | W | Chattanooga FC 2 at Inter Miami CF II 1 | Final |
| March 15, 2025 | W | Huntsville City Football Club 0 at Chattanooga FC 1 | Final |
| March 30, 2025 | T | Chattanooga FC 2 at Atlanta United 2 2 | Final (PK 4-3) |
| April 5, 2025 | W | Orlando City B 0 at Chattanooga FC 2 | Final |
| April 13, 2025 | W | Chattanooga FC 4 at Carolina Core FC 1 | Final |
| April 19, 2025 | W | Crown Legacy FC 1 at Chattanooga FC 2 | Final |
| April 27, 2025 | L | Chattanooga FC 0 at Columbus Crew 2 3 | Final |
| May 3, 2025 | T | Philadelphia Union II 0 at Chattanooga FC 0 | Final (PK 9-8) |
| May 9, 2025 | W | Chattanooga FC 2 at Toronto FC II 1 | Final |
| May 17, 2025 | W | Chattanooga FC 2 at Huntsville City Football Club 1 | Final |
| May 24, 2025 | W | New England Revolution II 0 at Chattanooga FC 1 | Final |
| June 1, 2025 | L | Chattanooga FC 1 at Orlando City B 2 | Final |
| June 7, 2025 | T | Inter Miami CF II 3 at Chattanooga FC 3 | Final (PK 4-5) |
| June 15, 2025 | L | Chattanooga FC 2 at New York Red Bulls II 5 | Final |
| June 21, 2025 | T | FC Cincinnati 2 0 at Chattanooga FC 0 | Final (PK 8-7) |
| July 5, 2025 | L | Chicago Fire FC II 1 at Chattanooga FC 0 | Final |
| July 13, 2025 | T | Chattanooga FC 3 at Philadelphia Union II 3 | Final (PK 5-6) |
| July 19, 2025 | T | Inter Miami CF II 2 at Chattanooga FC 2 | Final (PK 4-5) |
| July 26, 2025 | L | Crown Legacy FC 3 at Chattanooga FC 1 | Final |
| August 2, 2025 | L | Toronto FC II 2 at Chattanooga FC 1 | Final |
| August 9, 2025 | T | Chattanooga FC 2 at Orlando City B 2 | Final (PK 4-2) |
| August 16, 2025 | W | Chattanooga FC 1 at Carolina Core FC 0 | Final |
| August 23, 2025 | W | Huntsville City Football Club 0 at Chattanooga FC 1 | Final |
| September 12, 2025 | W | Chattanooga FC 4 at Crown Legacy FC 2 | Final |
| September 21, 2025 | T | Carolina Core FC 0 at Chattanooga FC 0 | Final (PK 6-5) |
| September 24, 2025 | | Chattanooga FC at Atlanta United 2 | 7:30 p.m. |
| September 28, 2025 | | Chattanooga FC at New York City FC II | 7:30 p.m. |
| October 5, 2025 | | Atlanta United 2 at Chattanooga FC | 1:00 p.m. |
Chattanooga FC Schedule
