| | | |
| March 9, 2025 | W | Huntsville City Football Club 4 at Chicago Fire FC II 1 | Final |
| March 15, 2025 | L | Huntsville City Football Club 0 at Chattanooga FC 1 | Final |
| March 28, 2025 | T | Philadelphia Union II 2 at Huntsville City Football Club 2 | Final (PK 3-4) |
| April 13, 2025 | T | Huntsville City Football Club 1 at Crown Legacy FC 1 | Final (PK 4-3) |
| April 20, 2025 | W | Chicago Fire FC II 0 at Huntsville City Football Club 5 | Final |
| April 27, 2025 | W | Inter Miami CF II 0 at Huntsville City Football Club 4 | Final |
| May 3, 2025 | T | Atlanta United 2 2 at Huntsville City Football Club 2 | Final (PK 5-4) |
| May 10, 2025 | T | Huntsville City Football Club 0 at Carolina Core FC 0 | Final (PK 3-2) |
| May 17, 2025 | L | Chattanooga FC 2 at Huntsville City Football Club 1 | Final |
| May 23, 2025 | W | Huntsville City Football Club 2 at Toronto FC II 1 | Final |
| June 6, 2025 | L | Orlando City B 3 at Huntsville City Football Club 2 | Final |
| June 11, 2025 | W | Huntsville City Football Club 1 at Atlanta United 2 0 | Final |
| June 20, 2025 | T | Crown Legacy FC 3 at Huntsville City Football Club 3 | Final (PK 4-3) |
| June 29, 2025 | | Huntsville City Football Club at New England Revolution II | Ppd. |
| July 6, 2025 | W | Huntsville City Football Club 2 at New York Red Bulls II 1 | Final |
| July 13, 2025 | L | Huntsville City Football Club 0 at FC Cincinnati 2 1 | Final |
| July 18, 2025 | W | Columbus Crew 2 1 at Huntsville City Football Club 2 | Final |
| July 25, 2025 | W | Orlando City B 0 at Huntsville City Football Club 4 | Final |
| August 2, 2025 | W | Huntsville City Football Club 4 at Crown Legacy FC 0 | Final |
| August 10, 2025 | W | Carolina Core FC 2 at Huntsville City Football Club 3 | Final |
| August 14, 2025 | T | Huntsville City Football Club 2 at Inter Miami CF II 2 | Final (PK 3-1) |
| August 17, 2025 | W | New York City FC II 1 at Huntsville City Football Club 2 | Final |
| August 23, 2025 | L | Huntsville City Football Club 0 at Chattanooga FC 1 | Final |
| August 30, 2025 | L | Huntsville City Football Club 0 at Atlanta United 2 2 | Final |
| September 8, 2025 | L | Huntsville City Football Club 0 at New England Revolution II 1 | Final |
| September 13, 2025 | L | New York Red Bulls II 3 at Huntsville City Football Club 2 | Final |
| September 21, 2025 | | Huntsville City Football Club at Orlando City B | 7:00 p.m. |
| September 28, 2025 | | Inter Miami CF II at Huntsville City Football Club | 8:00 p.m. |
| October 5, 2025 | | Carolina Core FC at Huntsville City Football Club | 1:00 p.m. |
Huntsville City Football Club Schedule
