| | | |
| February 15, 2025 | W | Old Glory DC 31 at Miami Sharks 29 | Final |
| March 1, 2025 | L | San Diego Legion 26 at Old Glory DC 10 | Final |
| March 8, 2025 | W | Anthem Rugby Carolina 22 at Old Glory DC 26 | Final |
| March 15, 2025 | L | Old Glory DC 26 at Chicago Hounds 30 | Final |
| March 22, 2025 | W | New England Free Jacks 14 at Old Glory DC 28 | Final |
| March 29, 2025 | L | Old Glory DC 44 at Rugby FC Los Angeles 54 | Final |
| April 5, 2025 | W | Seattle Seawolves 12 at Old Glory DC 29 | Final |
| April 12, 2025 | L | Old Glory DC 29 at NOLA Gold 51 | Final |
| April 19, 2025 | L | Miami Sharks 37 at Old Glory DC 35 | Final |
| April 26, 2025 | L | Old Glory DC 27 at Houston SaberCats 48 | Final |
| May 4, 2025 | W | Utah Warriors 12 at Old Glory DC 28 | Final |
| May 13, 2025 | W | NOLA Gold 14 at Old Glory DC 27 | Final |
| May 18, 2025 | W | Old Glory DC 41 at Anthem Rugby Carolina 19 | Final |
| May 24, 2025 | W | Old Glory DC 20 at New England Free Jacks 17 | Final |
| May 31, 2025 | L | Rugby FC Los Angeles 43 at Old Glory DC 32 | Final |
| June 7, 2025 | L | Old Glory DC 5 at San Diego Legion 50 | Final |
| June 14, 2025 | L | Old Glory DC 16 at Chicago Hounds 27 (Playoff) | Final |
Old Glory DC Schedule
