| | | |
| March 9, 2025 | T | Philadelphia Union II 0 at New England Revolution II 0 | Final (PK 3-4) |
| March 17, 2025 | T | New York Red Bulls II 1 at Philadelphia Union II 1 | Final (PK 1-3) |
| March 28, 2025 | T | Philadelphia Union II 2 at Huntsville City Football Club 2 | Final (PK 3-4) |
| April 10, 2025 | W | Atlanta United 2 1 at Philadelphia Union II 5 | Final |
| April 23, 2025 | W | Philadelphia Union II 4 at New York City FC II 1 | Final |
| May 3, 2025 | T | Philadelphia Union II 0 at Chattanooga FC 0 | Final (PK 9-8) |
| May 11, 2025 | W | Columbus Crew 2 0 at Philadelphia Union II 3 | Final |
| May 25, 2025 | L | Inter Miami CF II 1 at Philadelphia Union II 0 | Final |
| June 3, 2025 | W | Philadelphia Union II 4 at FC Cincinnati 2 1 | Final |
| June 15, 2025 | W | Crown Legacy FC 0 at Philadelphia Union II 3 | Final |
| June 21, 2025 | W | Philadelphia Union II 3 at Columbus Crew 2 1 | Final |
| June 26, 2025 | T | New England Revolution II 2 at Philadelphia Union II 2 | Final (PK 6-7) |
| June 30, 2025 | T | Orlando City B 1 at Philadelphia Union II 1 | Final (PK 2-4) |
| July 6, 2025 | W | Toronto FC II 0 at Philadelphia Union II 5 | Final |
| July 13, 2025 | T | Chattanooga FC 3 at Philadelphia Union II 3 | Final (PK 5-6) |
| July 19, 2025 | W | Philadelphia Union II 4 at Crown Legacy FC 0 | Final |
| July 26, 2025 | W | Philadelphia Union II 2 at New York City FC II 0 | Final |
| July 30, 2025 | L | Philadelphia Union II 2 at Carolina Core FC 3 | Final |
| August 3, 2025 | W | FC Cincinnati 2 0 at Philadelphia Union II 1 | Final |
| August 11, 2025 | L | Philadelphia Union II 0 at New York Red Bulls II 2 | Final |
| August 15, 2025 | L | Philadelphia Union II 0 at Chicago Fire FC II 2 | Final |
| August 20, 2025 | T | Toronto FC II 2 at Philadelphia Union II 2 | Final (PK 8-7) |
| August 24, 2025 | L | New England Revolution II 3 at Philadelphia Union II 1 | Final |
| August 31, 2025 | W | Philadelphia Union II 2 at FC Cincinnati 2 1 | Final |
| September 14, 2025 | W | Philadelphia Union II 3 at Columbus Crew 2 1 | Final |
| September 21, 2025 | W | Chicago Fire FC II 2 at Philadelphia Union II 5 | Final |
| September 26, 2025 | W | Philadelphia Union II 2 at Toronto FC II 1 | Final |
| October 5, 2025 | W | New York City FC II 3 at Philadelphia Union II 4 | Final |
| October 19, 2025 | W | FC Cincinnati 2 1 at Philadelphia Union II 2 (Playoff) | Final |
| October 25, 2025 | W | Huntsville City Football Club 0 at Philadelphia Union II 1 (Playoff) | Final |
| November 1, 2025 | L | Philadelphia Union II 1 at New York Red Bulls II 2 (Playoff) | Final |
Philadelphia Union II Schedule
