| | | |
| March 9, 2025 | W | Toronto FC II 1 at FC Cincinnati 2 0 | Final |
| March 13, 2025 | L | Toronto FC II 0 at Chicago Fire FC II 1 | Final |
| March 28, 2025 | L | Toronto FC II 0 at New England Revolution II 4 | Final |
| April 10, 2025 | W | Toronto FC II 1 at Inter Miami CF II 0 | Final |
| April 18, 2025 | T | Carolina Core FC 1 at Toronto FC II 1 | Final (PK 4-1) |
| April 25, 2025 | W | Chicago Fire FC II 0 at Toronto FC II 2 | Final |
| May 4, 2025 | T | New England Revolution II 3 at Toronto FC II 3 | Final (PK 9-8) |
| May 9, 2025 | L | Chattanooga FC 2 at Toronto FC II 1 | Final |
| May 15, 2025 | L | Toronto FC II 0 at New York City FC II 3 | Final |
| May 23, 2025 | L | Huntsville City Football Club 2 at Toronto FC II 1 | Final |
| May 30, 2025 | W | Crown Legacy FC 0 at Toronto FC II 1 | Final |
| June 8, 2025 | T | Toronto FC II 1 at FC Cincinnati 2 1 | Final (PK 2-4) |
| June 12, 2025 | W | Inter Miami CF II 0 at Toronto FC II 2 | Final |
| June 25, 2025 | W | Toronto FC II 2 at Orlando City B 1 | Final |
| July 6, 2025 | L | Toronto FC II 0 at Philadelphia Union II 5 | Final |
| July 11, 2025 | T | New York Red Bulls II 2 at Toronto FC II 2 | Final (PK 4-3) |
| July 16, 2025 | L | Toronto FC II 0 at New York City FC II 1 | Final |
| July 20, 2025 | L | Chicago Fire FC II 3 at Toronto FC II 2 | Final |
| July 27, 2025 | W | Toronto FC II 2 at Columbus Crew 2 1 | Final |
| August 2, 2025 | W | Toronto FC II 2 at Chattanooga FC 1 | Final |
| August 8, 2025 | L | Atlanta United 2 3 at Toronto FC II 1 | Final |
| August 15, 2025 | W | FC Cincinnati 2 0 at Toronto FC II 1 | Final |
| August 20, 2025 | | Toronto FC II at Philadelphia Union II | 7:00 p.m. |
| August 31, 2025 | | New York City FC II at Toronto FC II | 3:00 p.m. |
| September 12, 2025 | | Toronto FC II at New England Revolution II | 3:00 p.m. |
| September 19, 2025 | | Columbus Crew 2 at Toronto FC II | 7:00 p.m. |
| September 26, 2025 | | Philadelphia Union II at Toronto FC II | 7:00 p.m. |
| October 5, 2025 | | Toronto FC II at New York Red Bulls II | 1:00 p.m. |
Toronto FC II Schedule
