| | | |
| March 25, 2022 | L | Rochester New York FC 0 at St. Louis City SC 2 2 | Final |
| April 3, 2022 | L | Rochester New York FC 1 at New England Revolution II 3 | Final |
| April 10, 2022 | T | Rochester New York FC 0 at Chicago Fire FC II 0 | Final (PK 6-5) |
| April 23, 2022 | L | New York City FC II 3 at Rochester New York FC 2 | Final |
| April 29, 2022 | | Orlando City B at Rochester New York FC | Ppd. |
| May 8, 2022 | W | Rochester New York FC 2 at FC Cincinnati 2 1 | Final |
| May 14, 2022 | W | Inter Miami CF II 1 at Rochester New York FC 4 | Final |
| May 21, 2022 | W | Philadelphia Union II 0 at Rochester New York FC 3 | Final |
| May 29, 2022 | W | Rochester New York FC 4 at Columbus Crew 2 0 | Final |
| June 2, 2022 | T | Toronto FC II 1 at Rochester New York FC 1 | Final (PK 3-4) |
| June 11, 2022 | L | Rochester New York FC 0 at FC Cincinnati 2 1 | Final |
| June 18, 2022 | T | Rochester New York FC 1 at New England Revolution II 1 | Final (PK 5-4) |
| June 24, 2022 | T | Columbus Crew 2 0 at Rochester New York FC 0 | Final (PK 2-4) |
| July 7, 2022 | W | Philadelphia Union II 0 at Rochester New York FC 3 | Final |
| July 16, 2022 | W | Rochester New York FC 1 at New York City FC II 0 | Final |
| July 22, 2022 | L | FC Cincinnati 2 2 at Rochester New York FC 0 | Final |
| July 30, 2022 | T | Chicago Fire FC II 1 at Rochester New York FC 1 | Final (PK 5-4) |
| August 12, 2022 | L | Toronto FC II 2 at Rochester New York FC 1 | Final |
| August 17, 2022 | L | Rochester New York FC 2 at Orlando City B 5 | Final |
| August 21, 2022 | L | Rochester New York FC 1 at Inter Miami CF II 2 | Final |
| August 27, 2022 | W | New England Revolution II 0 at Rochester New York FC 2 | Final |
| September 4, 2022 | W | Rochester New York FC 2 at Philadelphia Union II 1 | Final |
| September 11, 2022 | W | Rochester New York FC 1 at Toronto FC II 0 | Final |
| September 18, 2022 | T | New York City FC II 2 at Rochester New York FC 2 | Final (PK 5-3) |
| September 24, 2022 | L | Rochester New York FC 1 at Columbus Crew 2 4 (Playoff) | Final |
Rochester New York FC Schedule
