| | | |
| March 1, 2026 | L | Connecticut United FC 3 at FC Cincinnati 2 1 | Final |
| March 8, 2026 | L | FC Cincinnati 2 0 at Philadelphia Union II 1 | Final |
| March 15, 2026 | L | FC Cincinnati 2 1 at New England Revolution II 2 | Final |
| March 21, 2026 | L | FC Cincinnati 2 0 at Columbus Crew 2 2 | Final |
| April 13, 2026 | W | New York City FC II 0 at FC Cincinnati 2 1 | Final |
| April 19, 2026 | L | FC Cincinnati 2 1 at Red Bull New York II 3 | Final |
| May 3, 2026 | W | Toronto FC II 0 at FC Cincinnati 2 5 | Final |
| May 10, 2026 | W | Columbus Crew 2 1 at FC Cincinnati 2 2 | Final |
| May 13, 2026 | L | FC Cincinnati 2 0 at Huntsville City FC 4 | Final |
| May 17, 2026 | L | Chattanooga FC 3 at FC Cincinnati 2 1 | Final |
| May 23, 2026 | L | FC Cincinnati 2 0 at New York City FC II 2 | Final |
| June 7, 2026 | L | New England Revolution II 1 at FC Cincinnati 2 0 | Final |
| June 14, 2026 | L | Columbus Crew 2 1 at FC Cincinnati 2 0 | Final |
| June 18, 2026 | L | FC Cincinnati 2 0 at Connecticut United FC 4 | Final |
| June 28, 2026 | | Inter Miami CF II at FC Cincinnati 2 | 6:00 p.m. |
| July 5, 2026 | | Red Bull New York II at FC Cincinnati 2 | 6:00 p.m. |
| July 12, 2026 | | Orlando City B at FC Cincinnati 2 | 6:00 p.m. |
| July 17, 2026 | | FC Cincinnati 2 at Toronto FC II | 7:00 p.m. |
| July 26, 2026 | | New England Revolution II at FC Cincinnati 2 | 7:00 p.m. |
| August 2, 2026 | | FC Cincinnati 2 at Chicago Fire FC II | 4:00 p.m. |
| August 8, 2026 | | FC Cincinnati 2 at Philadelphia Union II | 12:00 p.m. |
| August 16, 2026 | | Connecticut United FC at FC Cincinnati 2 | 6:00 p.m. |
| August 19, 2026 | | FC Cincinnati 2 at New York City FC II | 5:00 p.m. |
| August 22, 2026 | | FC Cincinnati 2 at Carolina Core FC | 7:30 p.m. |
| August 30, 2026 | | Philadelphia Union II at FC Cincinnati 2 | 6:00 p.m. |
| September 4, 2026 | | FC Cincinnati 2 at Toronto FC II | 7:00 p.m. |
| September 13, 2026 | | FC Cincinnati 2 at Red Bull New York II | 7:00 p.m. |
| September 20, 2026 | | Crown Legacy FC at FC Cincinnati 2 | 2:00 p.m. |
FC Cincinnati 2 Schedule
