| | | |
| March 9, 2025 | L | Toronto FC II 1 at FC Cincinnati 2 0 | Final |
| March 13, 2025 | L | FC Cincinnati 2 0 at New England Revolution II 2 | Final |
| March 30, 2025 | L | Chicago Fire FC II 2 at FC Cincinnati 2 1 | Final |
| April 9, 2025 | T | FC Cincinnati 2 2 at New York City FC II 2 | Final (PK 5-3) |
| April 20, 2025 | W | New York Red Bulls II 1 at FC Cincinnati 2 3 | Final |
| April 27, 2025 | T | Atlanta United 2 0 at FC Cincinnati 2 0 | Final (PK 3-5) |
| May 9, 2025 | L | FC Cincinnati 2 1 at Chicago Fire FC II 2 | Final |
| May 18, 2025 | L | FC Cincinnati 2 0 at Columbus Crew 2 1 | Final |
| May 25, 2025 | L | FC Cincinnati 2 0 at Crown Legacy FC 2 | Final |
| June 3, 2025 | L | Philadelphia Union II 4 at FC Cincinnati 2 1 | Final |
| June 8, 2025 | T | Toronto FC II 1 at FC Cincinnati 2 1 | Final (PK 2-4) |
| June 15, 2025 | W | FC Cincinnati 2 1 at New England Revolution II 0 | Final |
| June 21, 2025 | T | FC Cincinnati 2 0 at Chattanooga FC 0 | Final (PK 8-7) |
| June 29, 2025 | T | FC Cincinnati 2 3 at New York Red Bulls II 3 | Final (PK 5-3) |
| July 6, 2025 | T | New York City FC II 2 at FC Cincinnati 2 2 | Final (PK 4-5) |
| July 9, 2025 | T | FC Cincinnati 2 0 at Atlanta United 2 0 | Final (PK 4-3) |
| July 13, 2025 | W | Huntsville City Football Club 0 at FC Cincinnati 2 1 | Final |
| July 27, 2025 | W | FC Cincinnati 2 3 at Inter Miami CF II 0 | Final |
| August 3, 2025 | L | FC Cincinnati 2 0 at Philadelphia Union II 1 | Final |
| August 11, 2025 | W | Crown Legacy FC 3 at FC Cincinnati 2 4 | Final |
| August 15, 2025 | L | FC Cincinnati 2 0 at Toronto FC II 1 | Final |
| August 24, 2025 | L | Carolina Core FC 3 at FC Cincinnati 2 2 | Final |
| August 31, 2025 | L | Philadelphia Union II 2 at FC Cincinnati 2 1 | Final |
| September 10, 2025 | | FC Cincinnati 2 at Chicago Fire FC II | 8:00 p.m. |
| September 21, 2025 | | New England Revolution II at FC Cincinnati 2 | 6:00 p.m. |
| September 25, 2025 | | Columbus Crew 2 at FC Cincinnati 2 | 7:30 p.m. |
| October 1, 2025 | | New York Red Bulls II at FC Cincinnati 2 | 7:30 p.m. |
| October 5, 2025 | | FC Cincinnati 2 at Orlando City B | 1:00 p.m. |
FC Cincinnati 2 Schedule
