| | | |
| February 15, 2025 | L | Anthem Rugby Carolina 14 at NOLA Gold 35 | Final |
| February 21, 2025 | L | San Diego Legion 52 at Anthem Rugby Carolina 5 | Final |
| February 28, 2025 | L | Miami Sharks 32 at Anthem Rugby Carolina 31 | Final |
| March 8, 2025 | L | Anthem Rugby Carolina 22 at Old Glory DC 26 | Final |
| March 22, 2025 | L | Houston SaberCats 46 at Anthem Rugby Carolina 45 | Final |
| March 29, 2025 | W | Anthem Rugby Carolina 7 at Seattle Seawolves 3 | Final |
| April 5, 2025 | L | NOLA Gold 33 at Anthem Rugby Carolina 19 | Final |
| April 13, 2025 | L | Anthem Rugby Carolina 20 at Chicago Hounds 28 | Final |
| April 18, 2025 | L | New England Free Jacks 26 at Anthem Rugby Carolina 6 | Final |
| April 23, 2025 | L | Seattle Seawolves 31 at Anthem Rugby Carolina 14 | Final |
| April 27, 2025 | L | Anthem Rugby Carolina 5 at Miami Sharks 31 | Final |
| May 10, 2025 | L | Anthem Rugby Carolina 17 at Rugby FC Los Angeles 45 | Final |
| May 18, 2025 | L | Old Glory DC 41 at Anthem Rugby Carolina 19 | Final |
| May 24, 2025 | L | Chicago Hounds 33 at Anthem Rugby Carolina 19 | Final |
| May 30, 2025 | L | Anthem Rugby Carolina 10 at Utah Warriors 31 | Final |
| June 8, 2025 | L | Anthem Rugby Carolina 14 at Houston SaberCats 26 | Final |
Anthem Rugby Carolina Schedule
