| | | |
| March 25, 2024 | L | San Diego Strike Force 32 at Duke City Gladiators 26 | Final |
| March 29, 2024 | L | Duke City Gladiators 40 at Frisco Fighters 49 | Final |
| April 6, 2024 | L | Tucson Sugar Skulls 56 at Duke City Gladiators 49 | Final |
| April 20, 2024 | L | Duke City Gladiators 23 at Northern Arizona Wranglers 39 | Final |
| April 27, 2024 | L | Duke City Gladiators 27 at Vegas Knight Hawks 42 | Final |
| May 11, 2024 | L | San Antonio Gunslingers 56 at Duke City Gladiators 53 | Final |
| May 19, 2024 | L | Duke City Gladiators 21 at San Diego Strike Force 47 | Final |
| May 25, 2024 | L | Duke City Gladiators 53 at Arizona Rattlers 55 | Final |
| June 1, 2024 | W | Duke City Gladiators 21 at Tucson Sugar Skulls 20 | Final |
| June 8, 2024 | L | Duke City Gladiators 45 at San Antonio Gunslingers 46 | Final |
| June 15, 2024 | L | Vegas Knight Hawks 53 at Duke City Gladiators 39 | Final |
| June 22, 2024 | L | Bay Area Panthers 34 at Duke City Gladiators 32 | Final |
| June 29, 2024 | W | Tucson Sugar Skulls 32 at Duke City Gladiators 44 | Final |
| July 7, 2024 | L | Frisco Fighters 81 at Duke City Gladiators 55 | Final |
| July 13, 2024 | L | Duke City Gladiators 28 at Bay Area Panthers 50 | Final |
| July 21, 2024 | W | Northern Arizona Wranglers 48 at Duke City Gladiators 69 | Final |
Duke City Gladiators Schedule
