| | | |
| March 27, 2022 | L | FC Cincinnati 2 0 at Philadelphia Union II 2 | Final |
| April 3, 2022 | W | Toronto FC II 1 at FC Cincinnati 2 2 | Final |
| April 17, 2022 | L | FC Cincinnati 2 2 at New England Revolution II 3 | Final |
| April 23, 2022 | L | Inter Miami CF II 1 at FC Cincinnati 2 0 | Final |
| April 30, 2022 | L | FC Cincinnati 2 2 at Toronto FC II 4 | Final |
| May 8, 2022 | L | Rochester New York FC 2 at FC Cincinnati 2 1 | Final |
| May 15, 2022 | L | FC Cincinnati 2 1 at Chicago Fire FC II 3 | Final |
| May 22, 2022 | L | New England Revolution II 1 at FC Cincinnati 2 0 | Final |
| May 29, 2022 | | FC Cincinnati 2 at Orlando City B | 7:00 p.m. |
| June 11, 2022 | | Rochester New York FC at FC Cincinnati 2 | 8:00 p.m. |
| June 19, 2022 | | FC Cincinnati 2 at Inter Miami CF II | 8:00 p.m. |
| June 25, 2022 | | Orlando City B at FC Cincinnati 2 | 1:00 p.m. |
| July 2, 2022 | | FC Cincinnati 2 at New York City FC II | 7:00 p.m. |
| July 10, 2022 | | Chicago Fire FC II at FC Cincinnati 2 | 6:00 p.m. |
| July 16, 2022 | | FC Cincinnati 2 at Columbus Crew 2 | 7:00 p.m. |
| July 23, 2022 | | FC Cincinnati 2 at Rochester New York FC | 5:30 p.m. |
| July 31, 2022 | | Inter Miami CF II at FC Cincinnati 2 | 6:00 p.m. |
| August 7, 2022 | | Philadelphia Union II at FC Cincinnati 2 | 6:00 p.m. |
| August 14, 2022 | | Columbus Crew 2 at FC Cincinnati 2 | 6:00 p.m. |
| August 21, 2022 | | FC Cincinnati 2 at Toronto FC II | 7:00 p.m. |
| August 29, 2022 | | FC Cincinnati 2 at Orlando City B | 7:00 p.m. |
| September 4, 2022 | | New York City FC II at FC Cincinnati 2 | 4:00 p.m. |
| September 11, 2022 | | Chicago Fire FC II at FC Cincinnati 2 | 4:00 p.m. |
| September 18, 2022 | | FC Cincinnati 2 at Columbus Crew 2 | 3:00 p.m. |
FC Cincinnati 2 Schedule
