| | | |
| March 16, 2024 | W | One Knoxville SC 2 at Charlotte Independence 1 | Final |
| March 23, 2024 | W | Lexington Sporting Club 0 at One Knoxville SC 2 | Final |
| March 30, 2024 | W | South Georgia Tormenta FC 0 at One Knoxville SC 1 | Final |
| April 12, 2024 | L | Union Omaha 1 at One Knoxville SC 0 | Final |
| April 27, 2024 | | One Knoxville SC at Chattanooga Red Wolves SC | 7:30 p.m. |
| May 3, 2024 | | One Knoxville SC at Richmond Kickers | 7:00 p.m. |
| May 8, 2024 | | Forward Madison FC at One Knoxville SC | 6:30 p.m. |
| May 11, 2024 | | Northern Colorado Hailstorm FC at One Knoxville SC | 7:00 p.m. |
| May 17, 2024 | | One Knoxville SC at Central Valley Fuego FC | 10:30 p.m. |
| May 25, 2024 | | One Knoxville SC at Forward Madison FC | 7:00 p.m. |
| June 1, 2024 | | Richmond Kickers at One Knoxville SC | 7:00 p.m. |
| June 8, 2024 | | Lexington Sporting Club at One Knoxville SC | 7:00 p.m. |
| June 17, 2024 | | One Knoxville SC at Northern Colorado Hailstorm FC | 9:00 p.m. |
| June 22, 2024 | | Greenville Triumph SC at One Knoxville SC | 7:00 p.m. |
| June 29, 2024 | | Forward Madison FC at One Knoxville SC | 7:30 p.m. |
| July 5, 2024 | | One Knoxville SC at South Georgia Tormenta FC | 7:30 p.m. |
| July 13, 2024 | | Northern Colorado Hailstorm FC at One Knoxville SC | 7:00 p.m. |
| July 20, 2024 | | Chattanooga Red Wolves SC at One Knoxville SC | 7:00 p.m. |
| July 27, 2024 | | One Knoxville SC at Greenville Triumph SC | 7:00 p.m. |
| August 10, 2024 | | One Knoxville SC at Lexington Sporting Club | 7:00 p.m. |
| August 16, 2024 | | Chattanooga Red Wolves SC at One Knoxville SC | 7:00 p.m. |
| August 23, 2024 | | Spokane Velocity FC at One Knoxville SC | 7:00 p.m. |
| September 1, 2024 | | One Knoxville SC at South Georgia Tormenta FC | 7:30 p.m. |
| September 7, 2024 | | Charlotte Independence at One Knoxville SC | 6:00 p.m. |
| September 14, 2024 | | One Knoxville SC at Lexington Sporting Club | 7:00 p.m. |
| September 21, 2024 | | One Knoxville SC at Union Omaha | 8:00 p.m. |
| October 5, 2024 | | One Knoxville SC at Chattanooga Red Wolves SC | 7:30 p.m. |
| October 15, 2024 | | Central Valley Fuego FC at One Knoxville SC | 7:00 p.m. |
| October 19, 2024 | | One Knoxville SC at Forward Madison FC | 7:00 p.m. |
| October 26, 2024 | | One Knoxville SC at Spokane Velocity FC | 10:00 p.m. |
One Knoxville SC Schedule
