| | | |
| March 16, 2025 | T | One Knoxville SC 2 at Spokane Velocity FC 2 | Final |
| March 22, 2025 | W | One Knoxville SC 2 at Texoma FC 0 | Final |
| April 9, 2025 | W | One Knoxville SC 1 at Greenville Triumph SC 0 | Final |
| April 19, 2025 | W | One Knoxville SC 1 at FC Naples 0 | Final |
| April 26, 2025 | T | FC Tulsa 2 at One Knoxville SC 2 (Jagermeister Cup) | Final |
| May 4, 2025 | T | One Knoxville SC 1 at Portland Hearts of Pine 1 | Final |
| May 9, 2025 | T | Forward Madison FC 1 at One Knoxville SC 1 | Final |
| May 16, 2025 | L | One Knoxville SC 1 at Charlotte Independence 3 | Final |
| May 24, 2025 | L | Indy Eleven 1 at One Knoxville SC 0 (Jagermeister Cup) | Final |
| June 7, 2025 | T | One Knoxville SC 1 at Westchester SC 1 | Final |
| June 13, 2025 | | Richmond Kickers 1 at One Knoxville SC 1 | Susp. |
| June 21, 2025 | W | South Georgia Tormenta FC 0 at One Knoxville SC 3 | Final |
| June 28, 2025 | L | One Knoxville SC 0 at Forward Madison FC 1 (Jagermeister Cup) | Final |
| July 5, 2025 | L | One Knoxville SC 0 at Chattanooga Red Wolves SC 1 | Final |
| July 12, 2025 | W | One Knoxville SC 2 at South Georgia Tormenta FC 1 | Final |
| July 16, 2025 | W | AV Alta FC 1 at One Knoxville SC 3 | Final |
| July 19, 2025 | T | Westchester SC 1 at One Knoxville SC 1 | Final |
| July 26, 2025 | L | One Knoxville SC 0 at Chattanooga Red Wolves SC 1 (Jagermeister Cup) | Final |
| July 30, 2025 | T | Charlotte Independence 2 at One Knoxville SC 2 | Final |
| August 2, 2025 | W | Portland Hearts of Pine 0 at One Knoxville SC 1 | Final |
| August 9, 2025 | T | One Knoxville SC 2 at Forward Madison FC 2 | Final |
| August 16, 2025 | W | Union Omaha 0 at One Knoxville SC 1 | Final |
| August 23, 2025 | L | One Knoxville SC 0 at Richmond Kickers 1 | Final |
| August 27, 2025 | T | FC Naples 0 at One Knoxville SC 0 | Final |
| August 30, 2025 | W | Spokane Velocity FC 0 at One Knoxville SC 1 | Final |
| September 6, 2025 | L | One Knoxville SC 1 at South Georgia Tormenta FC 2 | Final |
| September 13, 2025 | W | One Knoxville SC 2 at AV Alta FC 1 | Final |
| September 21, 2025 | T | One Knoxville SC 2 at Union Omaha 2 | Final |
| September 26, 2025 | W | Charlotte Independence 0 at One Knoxville SC 1 | Final |
| October 1, 2025 | W | Chattanooga Red Wolves SC 0 at One Knoxville SC 2 | Final |
| October 4, 2025 | L | One Knoxville SC 1 at Richmond Kickers 2 | Final |
| October 10, 2025 | W | Portland Hearts of Pine 1 at One Knoxville SC 3 | Final |
| October 19, 2025 | W | Texoma FC 1 at One Knoxville SC 2 | Final |
| October 25, 2025 | W | Greenville Triumph SC 0 at One Knoxville SC 1 | Final |
| November 2, 2025 | W | Charlotte Independence 1 at One Knoxville SC 2 (Playoff) | Final |
| November 8, 2025 | W | FC Naples 0 at One Knoxville SC 1 (Playoff) | Final |
| November 16, 2025 | | Spokane Velocity FC at One Knoxville SC (Championship) | 5:00 p.m. |
One Knoxville SC Schedule
